(Circular) (Six-base) MAPSORT of: pFN21AB7705.seq Check: 3443 from: 1 to: 5962 pFN21AB7705 5962 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2217 3305 1937 Size: 280 1088 4594 Fragments arranged by size: 4594 1088 280 Acc65I G'GTAC_C Cuts at: 2494 3283 2494 Size: 789 5173 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2114 4025 5409 5677 1632 Size: 482 1911 1384 268 1917 Fragments arranged by size: 1917 1911 1384 482 268 AflII C'TTAA_G Cuts at: 820 1017 2249 2521 2599 820 Size: 197 1232 272 78 4183 Fragments arranged by size: 4183 1232 272 197 78 AflIII A'CryG_T Cuts at: 1203 3259 4861 5838 1203 Size: 2056 1602 977 1327 Fragments arranged by size: 2056 1602 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 2319 1166 Size: 1153 4809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3091 3705 5277 3091 Size: 614 1572 3776 Fragments arranged by size: 3776 1572 614 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 3287 1870 Size: 73 1344 4545 Fragments arranged by size: 4545 1344 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2494 3283 4738 618 Size: 325 217 627 707 789 1455 1842 Fragments arranged by size: 1842 1455 789 707 627 325 217 BanII G_rGCy'C Cuts at: 1391 2927 3281 1391 Size: 1536 354 4072 Fragments arranged by size: 4072 1536 354 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2508 928 Size: 971 609 4382 Fragments arranged by size: 4382 971 609 BbvCI CC'TCA_GC Cuts at: 3084 3084 Size: 5962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 2152 2167 1548 Size: 604 15 5343 Fragments arranged by size: 5343 604 15 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 3013 4023 5179 1790 Size: 1223 1010 1156 2573 Fragments arranged by size: 2573 1223 1156 1010 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2946 2946 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2424 4622 1239 Size: 1185 2198 2579 Fragments arranged by size: 2579 2198 1185 Bpu10I CC'TnA_GC Cuts at: 3084 3084 Size: 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4625 882 Size: 617 3126 2219 Fragments arranged by size: 3126 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2080 3753 493 Size: 711 876 1673 2702 Fragments arranged by size: 2702 1673 876 711 BsaBI GATnn'nnATC Cuts at: 2151 5956 2151 Size: 3805 2157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3007 3037 1178 Size: 30 165 30 1604 30 4103 Fragments arranged by size: 4103 1604 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2917 1186 Size: 1731 4231 BseYI C'CCAG_C Cuts at: 3026 3055 3061 5165 5661 3026 Size: 29 6 2104 496 3327 Fragments arranged by size: 3327 2104 496 29 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3077 1163 Size: 573 1341 4048 Fragments arranged by size: 4048 1341 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2590 2927 3023 4023 4108 5179 2590 Size: 337 96 1000 85 1071 3373 Fragments arranged by size: 3373 1071 1000 337 96 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2590 2927 3013 3023 3281 4023 4108 Size: 399 800 337 86 10 258 742 85 Cuts at: 4108 5179 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 800 742 399 337 258 86 85 10 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2862 3851 5581 2862 Size: 989 1730 3243 Fragments arranged by size: 3243 1730 989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2978 3317 844 Size: 2134 339 3489 Fragments arranged by size: 3489 2134 339 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3070 4606 4793 1700 Size: 110 1260 1536 187 2869 Fragments arranged by size: 2869 1536 1260 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 3016 4022 5034 3016 Size: 1006 1012 3944 Fragments arranged by size: 3944 1012 1006 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957 Size: 1320 367 385 17 1452 86 12 346 Cuts at: 5957 1972 Size: 1977 Fragments arranged by size: 1977 1452 1320 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2613 513 Size: 552 939 609 3862 Fragments arranged by size: 3862 939 609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2452 3004 3097 3478 4272 4292 2452 Size: 552 93 381 794 20 4122 Fragments arranged by size: 4122 794 552 381 93 20 ClaI AT'CG_AT Cuts at: 1554 2335 1554 Size: 781 5181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2951 3409 4298 Size: 54 1425 50 275 18 1121 458 889 Cuts at: 4298 5927 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1121 889 458 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2578 3892 1353 Size: 780 445 1314 3423 Fragments arranged by size: 3423 1314 780 445 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2925 2925 Size: 5962 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2424 4025 4622 5409 5677 1239 Size: 393 482 310 1601 597 787 268 1524 Fragments arranged by size: 1601 1524 787 597 482 393 310 268 EcoO109I rG'GnC_Cy Cuts at: 2232 2728 3079 2232 Size: 496 351 5115 Fragments arranged by size: 5115 496 351 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2625 2625 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2186 2191 2218 2577 Size: 32 135 32 587 27 5 27 359 Cuts at: 2577 2609 4603 4635 4677 4709 1373 Size: 32 1994 32 42 32 2626 Fragments arranged by size: 2626 1994 587 359 135 42 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 2246 748 Size: 1498 4464 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 2498 3287 2498 Size: 789 5173 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2319 2419 3165 3920 4279 4438 518 Size: 648 1153 100 746 755 359 159 2042 Fragments arranged by size: 2042 1153 755 746 648 359 159 100 MspA1I CmG'CkG Cuts at: 1606 2442 3378 4055 5203 5448 1606 Size: 836 936 677 1148 245 2120 Fragments arranged by size: 2120 1148 936 836 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2613 513 Size: 552 939 609 3862 Fragments arranged by size: 3862 939 609 552 NdeI CA'TA_TG Cuts at: 387 2388 3150 387 Size: 2001 762 3199 Fragments arranged by size: 3199 2001 762 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 3263 3320 4865 1132 Size: 97 2034 57 1545 2229 Fragments arranged by size: 2229 2034 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 3259 4861 3259 Size: 1602 4360 PflMI CCAn_nnn'nTGG Cuts at: 1108 2714 1108 Size: 1606 4356 PfoI T'CCnGG_A Cuts at: 3075 3075 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PmlI CAC'GTG Cuts at: 2080 2080 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2232 2728 3079 2232 Size: 496 351 5115 Fragments arranged by size: 5115 496 351 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 2447 3314 830 Size: 1617 867 3478 Fragments arranged by size: 3478 1617 867 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SacI G_AGCT'C Cuts at: 2927 2927 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SanDI GG'GwC_CC Cuts at: 2728 2728 Size: 5962 SapI GCTCTTCn'nnn_ Cuts at: 2133 2578 2133 Size: 445 5517 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2443 2575 2974 3310 4445 5126 5317 Size: 219 1398 132 399 336 1135 681 191 Cuts at: 5317 826 Size: 1471 Fragments arranged by size: 1471 1398 1135 681 399 336 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 2521 2599 4066 4967 5229 Size: 197 926 306 272 78 1467 901 262 Cuts at: 5229 820 Size: 1553 Fragments arranged by size: 1553 1467 926 901 306 272 262 197 78 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 2613 2723 3401 513 Size: 552 939 186 423 110 678 3074 Fragments arranged by size: 3074 939 678 552 423 186 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2187 3989 4006 4159 4344 4789 Size: 1255 417 190 1802 17 153 185 445 Cuts at: 4789 325 Size: 1498 Fragments arranged by size: 1802 1498 1255 445 417 190 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3117 4208 5920 Size: 274 80 33 51 1443 1140 1091 1712 Cuts at: 5920 96 Size: 138 Fragments arranged by size: 1712 1443 1140 1091 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3007 3039 5922 5954 3007 Size: 32 2883 32 3015 Fragments arranged by size: 3015 2883 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI