(Circular) (Six-base) MAPSORT of: pFN21AB7703.seq Check: 2186 from: 1 to: 5953 pFN21AB7703 5953 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3036 3036 Size: 5953 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2403 3763 278 Size: 53 83 186 936 867 1360 2468 Fragments arranged by size: 2468 1360 936 867 186 83 53 AccI GT'mk_AC Cuts at: 1937 3296 1937 Size: 1359 4594 Acc65I G'GTAC_C Cuts at: 2803 3274 2803 Size: 471 5482 AclI AA'CG_TT Cuts at: 1563 3252 4080 4453 1563 Size: 1689 828 373 3063 Fragments arranged by size: 3063 1689 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2145 4016 5400 5668 1632 Size: 513 1871 1384 268 1917 Fragments arranged by size: 1917 1871 1384 513 268 AfeI AGC'GCT Cuts at: 2576 2576 Size: 5953 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 4852 5829 1203 Size: 3649 977 1327 Fragments arranged by size: 3649 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 3106 4682 3106 Size: 1576 4377 AleI CACnn'nnGTG Cuts at: 1166 3042 3132 1166 Size: 1876 90 3987 Fragments arranged by size: 3987 1876 90 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5953 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2813 2845 2813 Size: 32 5921 AlwNI CAG_nnn'CTG Cuts at: 2151 2711 2758 3696 5268 2151 Size: 560 47 938 1572 2836 Fragments arranged by size: 2836 1572 938 560 47 ApaI G_GGCC'C Cuts at: 2325 2624 2636 2325 Size: 299 12 5642 Fragments arranged by size: 5642 299 12 ApaLI G'TGCA_C Cuts at: 3209 4010 5166 3209 Size: 801 1156 3996 Fragments arranged by size: 3996 1156 801 ApoI r'AATT_y Cuts at: 1493 3262 3488 3640 5749 1493 Size: 1769 226 152 2109 1697 Fragments arranged by size: 2109 1769 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 2372 3278 1870 Size: 73 429 906 4545 Fragments arranged by size: 4545 906 429 73 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2210 2597 2612 2803 3274 Size: 325 217 627 423 387 15 191 471 Cuts at: 3274 4729 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 471 423 387 325 217 191 15 BanII G_rGCy'C Cuts at: 1391 2325 2624 2636 3272 1391 Size: 934 299 12 636 4072 Fragments arranged by size: 4072 934 636 299 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4982 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4144 4178 1903 Size: 34 2207 34 3678 Fragments arranged by size: 3678 2207 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 1548 Size: 5953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4564 136 Size: 107 122 71 631 605 2892 1525 Fragments arranged by size: 2892 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5948 5948 Size: 5953 BlpI GC'TnA_GC Cuts at: 3370 3370 Size: 5953 Bme1580I G_kGCm'C Cuts at: 1790 2213 2325 2617 2624 2636 2705 2796 2859 Size: 423 112 292 7 12 69 91 63 Cuts at: 2859 3213 4014 5170 1790 Size: 354 801 1156 2573 Fragments arranged by size: 2573 1156 801 423 354 292 112 91 69 63 12 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5953 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2051 2651 3684 Size: 416 53 83 399 24 634 600 1033 Cuts at: 3684 4642 442 Size: 958 1753 Fragments arranged by size: 1753 1033 958 634 600 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2056 2056 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2128 2447 2468 2487 3103 4613 1239 Size: 889 319 21 19 616 1510 2579 Fragments arranged by size: 2579 1510 889 616 319 21 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2160 3002 4078 4943 5241 2160 Size: 842 1076 865 298 2872 Fragments arranged by size: 2872 1076 865 842 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2915 4616 882 Size: 617 1416 1701 2219 Fragments arranged by size: 2219 1701 1416 617 BsaAI yAC'GTr Cuts at: 493 1204 3744 493 Size: 711 2540 2702 Fragments arranged by size: 2702 2540 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2400 3760 4142 4777 Size: 53 83 186 936 867 1360 382 635 Cuts at: 4777 275 Size: 1451 Fragments arranged by size: 1451 1360 936 867 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2813 2843 3176 3206 1178 Size: 30 165 30 1410 30 333 30 3925 Fragments arranged by size: 3925 1410 333 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2465 2800 2903 1186 Size: 1258 21 335 103 4236 Fragments arranged by size: 4236 1258 335 103 21 BseYI C'CCAG_C Cuts at: 2260 2528 2751 2941 5156 5652 2260 Size: 268 223 190 2215 496 2561 Fragments arranged by size: 2561 2215 496 268 223 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2625 3046 1163 Size: 573 889 421 4070 Fragments arranged by size: 4070 889 573 421 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3403 4164 5192 1540 Size: 275 186 1402 761 1028 2301 Fragments arranged by size: 2301 1402 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3213 4014 4099 5170 3213 Size: 801 85 1071 3996 Fragments arranged by size: 3996 1071 801 85 BsmI GAATG_Cn' Cuts at: 3464 3557 3464 Size: 93 5860 BsmBI CGTCTCn'nnnn_ Cuts at: 2125 2125 Size: 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2213 2325 2617 2624 2636 2705 2796 Size: 399 423 112 292 7 12 69 91 Cuts at: 2796 2859 3213 3272 4014 4099 5170 1391 Size: 63 354 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 423 399 354 292 112 91 85 69 63 59 12 7 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2832 3036 3308 844 Size: 1988 204 272 3489 Fragments arranged by size: 3489 1988 272 204 BsrBI CCG'CTC Cuts at: 1394 1823 3234 3840 1394 Size: 429 1411 606 3507 Fragments arranged by size: 3507 1411 606 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2844 2859 4448 4622 65 Size: 1183 1596 15 1589 174 1396 Fragments arranged by size: 1596 1589 1396 1183 174 15 BsrFI r'CCGG_y Cuts at: 1700 1810 2088 2906 3033 4597 4784 1700 Size: 110 278 818 127 1564 187 2869 Fragments arranged by size: 2869 1564 818 278 187 127 110 BsrGI T'GTAC_A Cuts at: 96 2997 96 Size: 2901 3052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 2789 4013 5025 2789 Size: 1224 1012 3717 Fragments arranged by size: 3717 1224 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2612 794 Size: 1818 4135 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstYI r'GATC_y Cuts at: 1972 2670 3283 3650 4035 4052 5504 5590 5602 Size: 698 613 367 385 17 1452 86 12 Cuts at: 5602 5948 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 698 613 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2484 2484 Size: 5953 BtgI C'CryG_G Cuts at: 513 1065 2004 2310 2405 3221 513 Size: 552 939 306 95 816 3245 Fragments arranged by size: 3245 939 816 552 306 95 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2151 487 Size: 683 56 925 4289 Fragments arranged by size: 4289 925 683 56 BtsI GCAGTG_nn' Cuts at: 2272 2308 3469 4263 4283 2272 Size: 36 1161 794 20 3942 Fragments arranged by size: 3942 1161 794 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DraIII CAC_nnn'GTG Cuts at: 2594 2594 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 3068 3400 Size: 54 1425 50 275 18 256 982 332 Cuts at: 3400 4289 5918 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 982 889 332 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2433 3883 1353 Size: 1080 1450 3423 Fragments arranged by size: 3423 1450 1080 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4540 4914 5060 387 Size: 1064 138 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2128 2145 2447 2468 2487 3103 4016 Size: 393 496 17 302 21 19 616 913 Cuts at: 4016 4613 5400 5668 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 913 787 616 597 496 393 302 268 21 19 17 EcoNI CCTnn'n_nnAGG Cuts at: 2329 2589 2329 Size: 260 5693 EcoO109I rG'GnC_Cy Cuts at: 2321 2322 2479 2620 2632 2633 2708 2885 2894 Size: 1 157 141 12 1 75 177 9 Cuts at: 2894 2321 Size: 5380 Fragments arranged by size: 5380 177 157 141 75 12 9 1 1 EcoRI G'AATT_C Cuts at: 3262 3640 3262 Size: 378 5575 FspI TGC'GCA Cuts at: 2843 4459 2843 Size: 1616 4337 FspAI rTGC'GCAy Cuts at: 2843 2843 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 2578 3124 5100 1678 Size: 900 546 1976 2531 Fragments arranged by size: 2531 1976 900 546 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3092 3124 3176 3208 4594 Size: 32 135 32 1520 32 52 32 1386 Cuts at: 4594 4626 4668 4700 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1520 1386 135 52 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2764 3297 3543 669 Size: 988 281 826 533 246 3079 Fragments arranged by size: 3079 988 826 533 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5953 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KpnI G_GTAC'C Cuts at: 2807 3278 2807 Size: 471 5482 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2838 2857 5067 5251 1305 Size: 1533 19 2210 184 2007 Fragments arranged by size: 2210 2007 1533 184 19 MscI TGG'CCA Cuts at: 10 64 1489 1832 3070 10 Size: 54 1425 343 1238 2893 Fragments arranged by size: 2893 1425 1238 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2657 2960 3042 3132 3206 3911 4270 Size: 648 1491 303 82 90 74 705 359 Cuts at: 4270 4429 518 Size: 159 2042 Fragments arranged by size: 2042 1491 705 648 359 303 159 90 82 74 MspA1I CmG'CkG Cuts at: 1606 2280 2339 2528 2565 3223 3369 4046 5194 Size: 674 59 189 37 658 146 677 1148 Cuts at: 5194 5439 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 674 658 245 189 146 59 37 NaeI GCC'GGC Cuts at: 2090 3035 2090 Size: 945 5008 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4462 Fragments arranged by size: 4462 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NgoMIV G'CCGG_C Cuts at: 2088 3033 2088 Size: 945 5008 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 2107 3311 4856 1132 Size: 97 878 1204 1545 2229 Fragments arranged by size: 2229 1545 1204 878 97 PasI CC'CwG_GG Cuts at: 1348 2617 2720 2890 1348 Size: 1269 103 170 4411 Fragments arranged by size: 4411 1269 170 103 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 2594 2758 1108 Size: 1486 164 4303 Fragments arranged by size: 4303 1486 164 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2813 2845 3155 3187 4022 4054 5535 5567 2813 Size: 32 310 32 835 32 1481 32 3199 Fragments arranged by size: 3199 1481 835 310 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2479 2885 2479 Size: 406 5547 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PspOMI G'GGCC_C Cuts at: 2321 2620 2632 2321 Size: 299 12 5642 Fragments arranged by size: 5642 299 12 PstI C_TGCA'G Cuts at: 830 2516 2670 2990 3305 830 Size: 1686 154 320 315 3478 Fragments arranged by size: 3478 1686 320 315 154 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 PvuII CAG'CTG Cuts at: 2528 2528 Size: 5953 SacII CC_GC'GG Cuts at: 3224 3224 Size: 5953 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SanDI GG'GwC_CC Cuts at: 2885 2885 Size: 5953 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 4201 5913 4201 Size: 1712 4241 SexAI A'CCwGG_T Cuts at: 2590 2590 Size: 5953 SfcI C'TryA_G Cuts at: 826 1045 2512 2666 2986 3301 4436 5117 5308 Size: 219 1467 154 320 315 1135 681 191 Cuts at: 5308 826 Size: 1471 Fragments arranged by size: 1471 1467 1135 681 320 315 219 191 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2175 3017 4057 4958 5220 820 Size: 197 926 232 842 1040 901 262 1553 Fragments arranged by size: 1553 1040 926 901 842 262 232 197 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 2205 1517 Size: 688 5265 StyI C'CwwG_G Cuts at: 513 1065 2004 3392 513 Size: 552 939 1388 3074 Fragments arranged by size: 3074 1388 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 2375 3980 3997 4150 4335 4780 Size: 1255 480 315 1605 17 153 185 445 Cuts at: 4780 325 Size: 1498 Fragments arranged by size: 1605 1498 1255 480 445 315 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2972 2997 4199 Size: 274 80 33 51 1443 995 25 1202 Cuts at: 4199 5911 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1202 995 274 138 80 51 33 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2397 2429 5913 5945 2397 Size: 32 3484 32 2405 Fragments arranged by size: 3484 2405 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2525 2552 2624 3078 2525 Size: 27 72 454 5400 Fragments arranged by size: 5400 454 72 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 2442 3053 3170 4082 2442 Size: 611 117 912 4313 Fragments arranged by size: 4313 912 611 117 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2401 3761 276 Size: 53 83 186 936 867 1360 2468 Fragments arranged by size: 2468 1360 936 867 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI