Restriction map for
pFN21AB7703
(Linear) (Six-base) MAPSORT of: KIBB7703.seq Check: 1789 from: 1 to: 1248
KIBB7703.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1031 1248
Size: 1031 217
AatII G_ACGT'C
Cuts at: 0 398 1248
Size: 398 850
Acc65I G'GTAC_C
Cuts at: 0 798 1248
Size: 798 450
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1248
Size: 140 1108
AfeI AGC'GCT
Cuts at: 0 571 1248
Size: 571 677
AhdI GACnn_n'nnGTC
Cuts at: 0 1101 1248
Size: 1101 147
AleI CACnn'nnGTG
Cuts at: 0 1037 1127 1248
Size: 1037 90 121
Fragments arranged by size:
1037 121 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 808 840 1248
Size: 808 32 408
Fragments arranged by size:
808 408 32
AlwNI CAG_nnn'CTG
Cuts at: 0 146 706 753 1248
Size: 146 560 47 495
Fragments arranged by size:
560 495 146 47
ApaI G_GGCC'C
Cuts at: 0 320 619 631 1248
Size: 320 299 12 617
Fragments arranged by size:
617 320 299 12
ApaLI G'TGCA_C
Cuts at: 0 1204 1248
Size: 1204 44
AvaI C'yCGr_G
Cuts at: 0 367 1248
Size: 367 881
BanI G'GyrC_C
Cuts at: 0 205 592 607 798 1248
Size: 205 387 15 191 450
Fragments arranged by size:
450 387 205 191 15
BanII G_rGCy'C
Cuts at: 0 320 619 631 1248
Size: 320 299 12 617
Fragments arranged by size:
617 320 299 12
Bme1580I G_kGCm'C
Cuts at: 0 208 320 612 619 631 700 791 854
Size: 208 112 292 7 12 69 91 63
Cuts at: 854 1208 1248
Size: 354 40
Fragments arranged by size:
354 292 208 112 91 69 63 40
12 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 46 646 1248
Size: 46 600 602
Fragments arranged by size:
602 600 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 51 1248
Size: 51 1197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 442 463 482 1098 1248
Size: 123 319 21 19 616 150
Fragments arranged by size:
616 319 150 123 21 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 997 1248
Size: 155 842 251
Fragments arranged by size:
842 251 155
BsaI GGTCTCn'nnnn_
Cuts at: 0 910 1248
Size: 910 338
BsaHI Gr'CG_yC
Cuts at: 0 395 1248
Size: 395 853
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 808 838 1171 1201 1248
Size: 808 30 333 30 47
Fragments arranged by size:
808 333 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 460 795 898 1248
Size: 439 21 335 103 350
Fragments arranged by size:
439 350 335 103 21
BseYI C'CCAG_C
Cuts at: 0 255 523 746 936 1248
Size: 255 268 223 190 312
Fragments arranged by size:
312 268 255 223 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1041 1248
Size: 620 421 207
Fragments arranged by size:
620 421 207
BsiHKAI G_wGCw'C
Cuts at: 0 1208 1248
Size: 1208 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 120 1248
Size: 120 1128
Bsp1286I G_dGCh'C
Cuts at: 0 208 320 612 619 631 700 791 854
Size: 208 112 292 7 12 69 91 63
Cuts at: 854 1208 1248
Size: 354 40
Fragments arranged by size:
354 292 208 112 91 69 63 40
12 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 827 1031 1248
Size: 827 204 217
Fragments arranged by size:
827 217 204
BsrBI CCG'CTC
Cuts at: 0 1229 1248
Size: 1229 19
BsrDI GCAATG_nn'
Cuts at: 0 839 854 1248
Size: 839 15 394
Fragments arranged by size:
839 394 15
BsrFI r'CCGG_y
Cuts at: 0 83 901 1028 1248
Size: 83 818 127 220
Fragments arranged by size:
818 220 127 83
BsrGI T'GTAC_A
Cuts at: 0 992 1248
Size: 992 256
BssSI C'ACGA_G
Cuts at: 0 784 1248
Size: 784 464
BstAPI GCAn_nnn'nTGC
Cuts at: 0 607 1248
Size: 607 641
BstYI r'GATC_y
Cuts at: 0 665 1248
Size: 665 583
Bsu36I CC'TnA_GG
Cuts at: 0 479 1248
Size: 479 769
BtgI C'CryG_G
Cuts at: 0 305 400 1216 1248
Size: 305 95 816 32
Fragments arranged by size:
816 305 95 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 146 1248
Size: 146 1102
BtsI GCAGTG_nn'
Cuts at: 0 267 303 1248
Size: 267 36 945
Fragments arranged by size:
945 267 36
DraIII CAC_nnn'GTG
Cuts at: 0 589 1248
Size: 589 659
EaeI y'GGCC_r
Cuts at: 0 81 1063 1248
Size: 81 982 185
Fragments arranged by size:
982 185 81
EarI CTCTTCn'nnn_
Cuts at: 0 428 1248
Size: 428 820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 140 442 463 482 1098 1248
Size: 123 17 302 21 19 616 150
Fragments arranged by size:
616 302 150 123 21 19 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 324 584 1248
Size: 324 260 664
Fragments arranged by size:
664 324 260
EcoO109I rG'GnC_Cy
Cuts at: 0 316 317 474 615 627 628 703 880
Size: 316 1 157 141 12 1 75 177
Cuts at: 880 889 1248
Size: 9 359
Fragments arranged by size:
359 316 177 157 141 75 12 9
1 1
FspI TGC'GCA
Cuts at: 0 838 1248
Size: 838 410
FspAI rTGC'GCAy
Cuts at: 0 838 1248
Size: 838 410
HaeII r_GCGC'y
Cuts at: 0 573 1119 1248
Size: 573 546 129
Fragments arranged by size:
573 546 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1087 1119 1171 1203 1248
Size: 1087 32 52 32 45
Fragments arranged by size:
1087 52 45 32 32
HincII GTy'rAC
Cuts at: 0 759 1248
Size: 759 489
KpnI G_GTAC'C
Cuts at: 0 802 1248
Size: 802 446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 852 1248
Size: 833 19 396
Fragments arranged by size:
833 396 19
MscI TGG'CCA
Cuts at: 0 1065 1248
Size: 1065 183
MslI CAynn'nnrTG
Cuts at: 0 652 955 1037 1127 1201 1248
Size: 652 303 82 90 74 47
Fragments arranged by size:
652 303 90 82 74 47
MspA1I CmG'CkG
Cuts at: 0 275 334 523 560 1218 1248
Size: 275 59 189 37 658 30
Fragments arranged by size:
658 275 189 59 37 30
NaeI GCC'GGC
Cuts at: 0 85 1030 1248
Size: 85 945 218
Fragments arranged by size:
945 218 85
NgoMIV G'CCGG_C
Cuts at: 0 83 1028 1248
Size: 83 945 220
Fragments arranged by size:
945 220 83
NspI r_CATG'y
Cuts at: 0 102 1248
Size: 102 1146
PasI CC'CwG_GG
Cuts at: 0 612 715 885 1248
Size: 612 103 170 363
Fragments arranged by size:
612 363 170 103
PflMI CCAn_nnn'nTGG
Cuts at: 0 589 753 1248
Size: 589 164 495
Fragments arranged by size:
589 495 164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 808 840 1150 1182 1248
Size: 808 32 310 32 66
Fragments arranged by size:
808 310 66 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 474 880 1248
Size: 474 406 368
Fragments arranged by size:
474 406 368
PspOMI G'GGCC_C
Cuts at: 0 316 615 627 1248
Size: 316 299 12 621
Fragments arranged by size:
621 316 299 12
PstI C_TGCA'G
Cuts at: 0 511 665 985 1248
Size: 511 154 320 263
Fragments arranged by size:
511 320 263 154
PvuII CAG'CTG
Cuts at: 0 523 1248
Size: 523 725
SacII CC_GC'GG
Cuts at: 0 1219 1248
Size: 1219 29
SanDI GG'GwC_CC
Cuts at: 0 880 1248
Size: 880 368
SexAI A'CCwGG_T
Cuts at: 0 585 1248
Size: 585 663
SfcI C'TryA_G
Cuts at: 0 507 661 981 1248
Size: 507 154 320 267
Fragments arranged by size:
507 320 267 154
SmlI C'TyrA_G
Cuts at: 0 170 1012 1248
Size: 170 842 236
Fragments arranged by size:
842 236 170
StuI AGG'CCT
Cuts at: 0 200 1248
Size: 200 1048
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 370 1248
Size: 55 315 878
Fragments arranged by size:
878 315 55
TatI w'GTAC_w
Cuts at: 0 967 992 1248
Size: 967 25 256
Fragments arranged by size:
967 256 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 392 424 1248
Size: 392 32 824
Fragments arranged by size:
824 392 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 520 547 619 1073 1248
Size: 520 27 72 454 175
Fragments arranged by size:
520 454 175 72 27
XmnI GAAnn'nnTTC
Cuts at: 0 437 1048 1165 1248
Size: 437 611 117 83
Fragments arranged by size:
611 437 117 83
ZraI GAC'GTC
Cuts at: 0 396 1248
Size: 396 852
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AhdI AleI AloI
AlwNI ApaI ApaLI AvaI BanI BanII Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PflMI PpiI PpuMI PspOMI
PstI PvuII SacII SanDI SexAI SfcI SmlI StuI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AlfI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BssHII BstBI BstEII BstXI BstZ17I ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI HindIII HpaI KasI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StyI SwaI Tth111I XbaI XhoI XmaI