(Circular) (Six-base) MAPSORT of: pFN21AB7702.seq Check: 3221 from: 1 to: 6088 pFN21AB7702 6088 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2482 2838 2482 Size: 356 5732 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3118 4151 5535 5803 1632 Size: 1486 1033 1384 268 1917 Fragments arranged by size: 1917 1486 1384 1033 268 AfeI AGC'GCT Cuts at: 2195 2195 Size: 6088 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 2090 2145 4987 5964 1203 Size: 887 55 2842 977 1327 Fragments arranged by size: 2842 1327 977 887 55 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 2062 4817 2062 Size: 2755 3333 AleI CACnn'nnGTG Cuts at: 1166 2848 1166 Size: 1682 4406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2866 2990 3096 3356 3371 1184 Size: 1682 124 106 260 15 3901 Fragments arranged by size: 3901 1682 260 124 106 15 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3134 3166 3134 Size: 32 6056 AlwNI CAG_nnn'CTG Cuts at: 2695 3064 3259 3262 3831 5403 2695 Size: 369 195 3 569 1572 3380 Fragments arranged by size: 3380 1572 569 369 195 3 ApaI G_GGCC'C Cuts at: 2317 2376 2503 2667 2731 3289 2317 Size: 59 127 164 64 558 5116 Fragments arranged by size: 5116 558 164 127 64 59 ApaLI G'TGCA_C Cuts at: 2149 4145 5301 2149 Size: 1996 1156 2936 Fragments arranged by size: 2936 1996 1156 ApoI r'AATT_y Cuts at: 1493 3248 3397 3623 3775 5884 1493 Size: 1755 149 226 152 2109 1697 Fragments arranged by size: 2109 1755 1697 226 152 149 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3413 1870 Size: 73 1470 4545 Fragments arranged by size: 4545 1470 73 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 2528 3409 4864 618 Size: 325 217 627 599 142 881 1455 1842 Fragments arranged by size: 1842 1455 881 627 599 325 217 142 BanII G_rGCy'C Cuts at: 1391 2317 2376 2451 2503 2652 2667 2682 2731 Size: 926 59 75 52 149 15 15 49 Cuts at: 2731 2757 3029 3289 3407 1391 Size: 26 272 260 118 4072 Fragments arranged by size: 4072 926 272 260 149 118 75 59 52 49 26 15 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2851 928 Size: 971 952 4165 Fragments arranged by size: 4165 971 952 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2368 2402 4279 4313 1903 Size: 34 431 34 1877 34 3678 Fragments arranged by size: 3678 1877 431 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3020 4699 136 Size: 107 122 71 631 605 1348 1679 1525 Fragments arranged by size: 1679 1525 1348 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 2045 2153 2317 2376 2391 2503 2526 2533 Size: 255 108 164 59 15 112 23 7 Cuts at: 2533 2667 2731 2845 3289 4149 5305 1790 Size: 134 64 114 444 860 1156 2573 Fragments arranged by size: 2573 1156 860 444 255 164 134 114 112 108 64 59 23 15 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2495 2848 3819 Size: 416 53 83 399 24 1078 353 971 Cuts at: 3819 4777 442 Size: 958 1753 Fragments arranged by size: 1753 1078 971 958 416 399 353 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 2679 2723 2891 2929 4748 1239 Size: 1145 295 44 168 38 1819 2579 Fragments arranged by size: 2579 1819 1145 295 168 44 38 Bpu10I CC'TnA_GC Cuts at: 2445 2676 3183 3290 2445 Size: 231 507 107 5243 Fragments arranged by size: 5243 507 231 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3101 4213 5078 5376 3101 Size: 1112 865 298 3813 Fragments arranged by size: 3813 1112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2091 2148 2419 3879 493 Size: 711 887 57 271 1460 2702 Fragments arranged by size: 2702 1460 887 711 271 57 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2410 4521 4919 5193 5340 5751 2410 Size: 2111 398 274 147 411 2747 Fragments arranged by size: 2747 2111 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3299 3329 1178 Size: 30 165 30 1896 30 3937 Fragments arranged by size: 3937 1896 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6088 BseYI C'CCAG_C Cuts at: 2429 2477 2503 5291 5787 2429 Size: 48 26 2788 496 2730 Fragments arranged by size: 2788 2730 496 48 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2821 3020 1163 Size: 573 411 674 199 4231 Fragments arranged by size: 4231 674 573 411 199 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2153 2682 4149 4234 5305 2153 Size: 529 1467 85 1071 2936 Fragments arranged by size: 2936 1467 1071 529 85 BsiWI C'GTAC_G Cuts at: 2016 2634 2016 Size: 618 5470 BsmI GAATG_Cn' Cuts at: 3599 3692 3599 Size: 93 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2153 2317 2376 2391 2451 2503 Size: 399 255 108 164 59 15 60 52 Cuts at: 2503 2526 2533 2652 2667 2682 2731 2757 2845 Size: 23 7 119 15 15 49 26 88 Cuts at: 2845 3029 3289 3407 4149 4234 5305 1391 Size: 184 260 118 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 260 255 184 164 119 118 108 88 85 60 59 52 49 26 23 15 15 15 7 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2482 2838 3363 3443 844 Size: 1638 356 525 80 3489 Fragments arranged by size: 3489 1638 525 356 80 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2465 2779 3096 4732 4919 1700 Size: 110 655 314 317 1636 187 2869 Fragments arranged by size: 2869 1636 655 317 314 187 110 BsrGI T'GTAC_A Cuts at: 96 2298 96 Size: 2202 3886 BssHII G'CGCG_C Cuts at: 1925 2254 1925 Size: 329 5759 BssSI C'ACGA_G Cuts at: 4148 5160 4148 Size: 1012 5076 BstAPI GCAn_nnn'nTGC Cuts at: 794 2695 3262 794 Size: 1901 567 3620 Fragments arranged by size: 3620 1901 567 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 2980 1184 Size: 1796 4292 BstXI CCAn_nnnn'nTGG Cuts at: 2458 2458 Size: 6088 BstYI r'GATC_y Cuts at: 1972 3418 3785 4170 4187 5639 5725 5737 6083 Size: 1446 367 385 17 1452 86 12 346 Cuts at: 6083 1972 Size: 1977 Fragments arranged by size: 1977 1452 1446 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2514 2533 513 Size: 552 939 510 19 4068 Fragments arranged by size: 4068 939 552 510 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 2507 3235 3604 4398 4418 2507 Size: 728 369 794 20 4177 Fragments arranged by size: 4177 794 728 369 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DraIII CAC_nnn'GTG Cuts at: 3222 3222 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2275 2460 2517 Size: 54 1425 50 275 18 445 185 57 Cuts at: 2517 2572 2808 2877 3012 3535 4424 6053 8 Size: 55 236 69 135 523 889 1629 43 Fragments arranged by size: 1629 1425 889 523 445 275 236 185 135 69 57 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3137 4018 1353 Size: 1784 881 3423 Fragments arranged by size: 3423 1784 881 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2680 2680 Size: 6088 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2384 2679 2723 2891 2929 3118 4151 Size: 393 752 295 44 168 38 189 1033 Cuts at: 4151 4748 5535 5803 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1033 787 752 597 393 295 268 189 168 44 38 EcoNI CCTnn'n_nnAGG Cuts at: 3275 3275 Size: 6088 EcoO109I rG'GnC_Cy Cuts at: 2026 2314 2372 2373 2455 2499 2500 2663 3286 Size: 288 58 1 82 44 1 163 623 Cuts at: 3286 2026 Size: 4828 Fragments arranged by size: 4828 623 288 163 82 58 44 1 1 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 6088 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2197 5235 1678 Size: 519 3038 2531 Fragments arranged by size: 3038 2531 519 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 4729 4761 4803 Size: 32 135 32 476 32 2649 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2649 2626 476 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3014 10 Size: 54 1425 343 1182 3084 Fragments arranged by size: 3084 1425 1182 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2519 2848 3170 3365 4046 4405 4564 Size: 648 1353 329 322 195 681 359 159 Cuts at: 4564 518 Size: 2042 Fragments arranged by size: 2042 1353 681 648 359 329 322 195 159 MspA1I CmG'CkG Cuts at: 1606 2692 2701 3076 3091 3259 3504 4181 5329 Size: 1086 9 375 15 168 245 677 1148 Cuts at: 5329 5574 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1086 677 375 245 245 168 15 9 NaeI GCC'GGC Cuts at: 2781 2781 Size: 6088 NcoI C'CATG_G Cuts at: 513 1065 2004 2514 513 Size: 552 939 510 4087 Fragments arranged by size: 4087 939 552 510 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 2779 2779 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 3298 3446 4991 1132 Size: 97 2069 148 1545 2229 Fragments arranged by size: 2229 2069 1545 148 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2477 2546 2624 3169 1108 Size: 1369 69 78 545 4027 Fragments arranged by size: 4027 1369 545 78 69 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PmlI CAC'GTG Cuts at: 2091 2148 2091 Size: 57 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 2026 Size: 6088 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 2313 2372 2499 2663 2727 3285 2313 Size: 59 127 164 64 558 5116 Fragments arranged by size: 5116 558 164 127 64 59 PstI C_TGCA'G Cuts at: 830 2691 2700 2751 2901 3075 3090 3440 830 Size: 1861 9 51 150 174 15 350 3478 Fragments arranged by size: 3478 1861 350 174 150 51 15 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 2692 2701 3076 3091 3259 2692 Size: 9 375 15 168 5521 Fragments arranged by size: 5521 375 168 15 9 SacI G_AGCT'C Cuts at: 2682 2682 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SbfI CC_TGCA'GG Cuts at: 2751 3440 2751 Size: 689 5399 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SexAI A'CCwGG_T Cuts at: 2718 2718 Size: 6088 SfcI C'TryA_G Cuts at: 826 1045 2687 2696 2747 2897 3071 3086 3436 Size: 219 1642 9 51 150 174 15 350 Cuts at: 3436 4571 5252 5443 826 Size: 1135 681 191 1471 Fragments arranged by size: 1642 1471 1135 681 350 219 191 174 150 51 15 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3116 4192 5093 5355 820 Size: 197 926 1173 1076 901 262 1553 Fragments arranged by size: 1553 1173 1076 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3298 3446 1132 Size: 2166 148 3774 Fragments arranged by size: 3774 2166 148 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2004 2451 2514 3303 3527 513 Size: 552 939 447 63 789 224 3074 Fragments arranged by size: 3074 939 789 552 447 224 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3032 4115 4132 4285 4470 4915 325 Size: 1255 1452 1083 17 153 185 445 1498 Fragments arranged by size: 1498 1452 1255 1083 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 4334 6046 Size: 274 80 33 51 1443 321 2036 1712 Cuts at: 6046 96 Size: 138 Fragments arranged by size: 2036 1712 1443 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2024 2056 6048 6080 2024 Size: 32 3992 32 2032 Fragments arranged by size: 3992 2032 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI