(Circular) (Six-base) MAPSORT of: pFN21AB7647.seq Check: 8513 from: 1 to: 5689 pFN21AB7647 5689 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 2394 3010 2394 Size: 616 5073 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5136 5404 1632 Size: 2120 1384 268 1917 Fragments arranged by size: 2120 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 2041 2210 4418 2041 Size: 169 2208 3312 Fragments arranged by size: 3312 2208 169 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2708 1184 Size: 1524 4165 AlwNI CAG_nnn'CTG Cuts at: 3432 5004 3432 Size: 1572 4117 ApaI G_GGCC'C Cuts at: 2418 2749 2418 Size: 331 5358 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2331 3014 1870 Size: 73 388 683 4545 Fragments arranged by size: 4545 683 388 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2394 3010 4465 618 Size: 325 217 627 607 616 1455 1842 Fragments arranged by size: 1842 1455 627 616 607 325 217 BanII G_rGCy'C Cuts at: 1391 2240 2338 2418 2749 3008 1391 Size: 849 98 80 331 259 4072 Fragments arranged by size: 4072 849 331 259 98 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 928 Size: 971 930 3788 Fragments arranged by size: 3788 971 930 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2563 2597 2758 2792 2800 2834 3880 Size: 34 626 34 161 34 8 34 1046 Cuts at: 3880 3914 1903 Size: 34 3678 Fragments arranged by size: 3678 1046 626 161 34 34 34 34 34 8 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2073 4300 136 Size: 107 122 71 631 605 401 2227 1525 Fragments arranged by size: 2227 1525 631 605 401 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 2081 3106 2081 Size: 1025 4664 Bme1580I G_kGCm'C Cuts at: 1790 2418 2604 2749 3750 4906 1790 Size: 628 186 145 1001 1156 2573 Fragments arranged by size: 2573 1156 1001 628 186 145 BmgBI CAC'GTC Cuts at: 1318 2062 1318 Size: 744 4945 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2712 3420 4378 Size: 416 53 83 399 24 1295 708 958 Cuts at: 4378 442 Size: 1753 Fragments arranged by size: 1753 1295 958 708 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2960 2960 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 4349 1239 Size: 1031 2079 2579 Fragments arranged by size: 2579 2079 1031 Bpu10I CC'TnA_GC Cuts at: 2570 2570 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2166 2965 4352 882 Size: 617 667 799 1387 2219 Fragments arranged by size: 2219 1387 799 667 617 BsaAI yAC'GTr Cuts at: 493 1204 2881 3480 493 Size: 711 1677 599 2702 Fragments arranged by size: 2702 1677 711 599 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2167 3496 3878 4513 Size: 53 83 186 936 634 1329 382 635 Cuts at: 4513 275 Size: 1451 Fragments arranged by size: 1451 1329 936 635 634 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2352 2439 4122 4520 4794 4941 5352 2352 Size: 87 1683 398 274 147 411 2689 Fragments arranged by size: 2689 1683 411 398 274 147 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2347 2377 2891 2921 1178 Size: 30 165 30 944 30 514 30 3946 Fragments arranged by size: 3946 944 514 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2617 1186 Size: 1431 4258 BseYI C'CCAG_C Cuts at: 2111 2417 2603 2647 2789 2921 4892 5388 2111 Size: 306 186 44 142 132 1971 496 2412 Fragments arranged by size: 2412 1971 496 306 186 142 132 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2448 1163 Size: 573 712 4404 Fragments arranged by size: 4404 712 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3750 3835 4906 3750 Size: 85 1071 4533 Fragments arranged by size: 4533 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2338 2418 2604 2749 3008 3750 Size: 399 450 98 80 186 145 259 742 Cuts at: 3750 3835 4906 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 399 259 186 145 98 85 80 BspEI T'CCGG_A Cuts at: 2352 2439 5352 2352 Size: 87 2913 2689 Fragments arranged by size: 2913 2689 87 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 844 Size: 2200 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2028 2663 3576 1394 Size: 429 205 635 913 3507 Fragments arranged by size: 3507 913 635 429 205 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2306 2062 Size: 244 5445 BstYI r'GATC_y Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 5684 1972 Size: 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2803 487 Size: 683 56 1577 3373 Fragments arranged by size: 3373 1577 683 56 BtsI GCAGTG_nn' Cuts at: 2217 3205 3999 4019 2217 Size: 988 794 20 3887 Fragments arranged by size: 3887 988 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2206 2241 529 Size: 35 1642 35 3977 Fragments arranged by size: 3977 1642 35 35 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 3136 4025 Size: 54 1425 50 275 18 261 1045 889 Cuts at: 4025 5654 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1045 889 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2122 2245 2590 3619 1353 Size: 769 123 345 1029 3423 Fragments arranged by size: 3423 1029 769 345 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 3752 4349 5136 5404 1239 Size: 393 638 1482 597 787 268 1524 Fragments arranged by size: 1524 1482 787 638 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2381 2381 Size: 5689 EcoO109I rG'GnC_Cy Cuts at: 2404 2414 2745 2404 Size: 10 331 5348 Fragments arranged by size: 5348 331 10 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 4836 1678 Size: 3158 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2163 2195 2196 Size: 32 135 32 455 32 104 32 1 Cuts at: 2196 2228 2266 2298 4330 4362 4404 4436 1373 Size: 32 38 32 2032 32 42 32 2626 Fragments arranged by size: 2626 2032 455 135 104 42 38 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2347 3033 3279 669 Size: 988 281 409 686 246 3079 Fragments arranged by size: 3079 988 686 409 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 2398 3014 2398 Size: 616 5073 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 2070 4803 4987 1305 Size: 753 12 2733 184 2007 Fragments arranged by size: 2733 2007 753 184 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 10 Size: 54 1425 343 261 3606 Fragments arranged by size: 3606 1425 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 3647 4006 4165 518 Size: 648 894 1587 359 159 2042 Fragments arranged by size: 2042 1587 894 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2101 2921 2933 3105 3782 4930 5175 Size: 474 21 820 12 172 677 1148 245 Cuts at: 5175 1606 Size: 2120 Fragments arranged by size: 2120 1148 820 677 474 245 172 21 12 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 2912 1348 Size: 1564 4125 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2404 2404 Size: 5689 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2414 2745 2414 Size: 331 5358 PstI C_TGCA'G Cuts at: 830 2835 3041 830 Size: 2005 206 3478 Fragments arranged by size: 3478 2005 206 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2080 2921 2080 Size: 841 4848 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SapI GCTCTTCn'nnn_ Cuts at: 2122 2245 2122 Size: 123 5566 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2831 2862 3037 4172 4853 5044 826 Size: 219 1786 31 175 1135 681 191 1471 Fragments arranged by size: 1786 1471 1135 681 219 191 175 31 SfiI GGCCn_nnn'nGGCC Cuts at: 2073 2073 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3793 4694 4956 820 Size: 197 926 1850 901 262 1553 Fragments arranged by size: 1850 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2016 3128 513 Size: 552 951 1112 3074 Fragments arranged by size: 3074 1112 951 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3716 3733 3886 4071 4516 325 Size: 1255 2136 17 153 185 445 1498 Fragments arranged by size: 2136 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3935 5647 96 Size: 274 80 33 51 1443 1958 1712 138 Fragments arranged by size: 1958 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5649 5681 5649 Size: 32 5657 Tth111I GACn'n_nGTC Cuts at: 1316 2435 1316 Size: 1119 4570 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 2509 2306 Size: 203 5486 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI