(Circular) (Six-base) MAPSORT of: pFN21AB7632.seq Check: 1139 from: 1 to: 5797 pFN21AB7632 5797 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2411 3140 1937 Size: 474 729 4594 Fragments arranged by size: 4594 729 474 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 2435 2697 3924 4297 1563 Size: 872 262 1227 373 3063 Fragments arranged by size: 3063 1227 872 373 262 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3860 5244 5512 1632 Size: 2228 1384 268 1917 Fragments arranged by size: 2228 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2290 820 Size: 197 1273 4327 Fragments arranged by size: 4327 1273 197 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 2892 4526 2892 Size: 1634 4163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2072 2104 2072 Size: 32 5765 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaI G_GGCC'C Cuts at: 2487 2634 3026 3066 2487 Size: 147 392 40 5218 Fragments arranged by size: 5218 392 147 40 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 2582 2627 2943 3066 3122 1870 Size: 73 639 45 316 123 56 4545 Fragments arranged by size: 4545 639 316 123 73 56 45 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 3118 4573 618 Size: 325 217 627 239 1092 1455 1842 Fragments arranged by size: 1842 1455 1092 627 325 239 217 BanII G_rGCy'C Cuts at: 1391 2487 2634 3026 3048 3066 3116 1391 Size: 1096 147 392 22 18 50 4072 Fragments arranged by size: 4072 1096 392 147 50 22 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2042 928 Size: 971 143 4683 Fragments arranged by size: 4683 971 143 BbvCI CC'TCA_GC Cuts at: 3013 3013 Size: 5797 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 2418 3214 2418 Size: 796 5001 Bme1580I G_kGCm'C Cuts at: 1790 2029 2487 2627 2634 3026 3066 3858 5014 Size: 239 458 140 7 392 40 792 1156 Cuts at: 5014 1790 Size: 2573 Fragments arranged by size: 2573 1156 792 458 392 239 140 40 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3528 4486 442 Size: 416 53 83 399 24 2111 958 1753 Fragments arranged by size: 2111 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2585 4457 1239 Size: 1346 1872 2579 Fragments arranged by size: 2579 1872 1346 Bpu10I CC'TnA_GC Cuts at: 3013 3042 3013 Size: 29 5768 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 3922 4787 5085 2267 Size: 1655 865 298 2979 Fragments arranged by size: 2979 1655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2792 3588 493 Size: 711 1588 796 2702 Fragments arranged by size: 2702 1588 796 711 BsaBI GATnn'nnATC Cuts at: 2139 5791 2139 Size: 3652 2145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 3986 4621 275 Size: 53 83 186 936 2071 382 635 1451 Fragments arranged by size: 2071 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2126 4230 4628 4902 5049 5460 2126 Size: 2104 398 274 147 411 2463 Fragments arranged by size: 2463 2104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5572 Fragments arranged by size: 5572 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2736 1186 Size: 904 646 4247 Fragments arranged by size: 4247 904 646 BseYI C'CCAG_C Cuts at: 2015 2042 2753 5000 5496 2015 Size: 27 711 2247 496 2316 Fragments arranged by size: 2316 2247 711 496 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3858 3943 5014 3858 Size: 85 1071 4641 Fragments arranged by size: 4641 1071 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2487 2627 2634 3026 3048 3066 Size: 399 239 458 140 7 392 22 18 Cuts at: 3066 3116 3858 3943 5014 1391 Size: 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 458 399 392 239 140 85 50 22 18 7 BspEI T'CCGG_A Cuts at: 2126 5460 2126 Size: 3334 2463 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2593 3152 844 Size: 1749 559 3489 Fragments arranged by size: 3489 1749 559 BsrBI CCG'CTC Cuts at: 1394 1823 2497 2578 3684 1394 Size: 429 674 81 1106 3507 Fragments arranged by size: 3507 1106 674 429 81 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2049 4441 4628 1700 Size: 110 239 2392 187 2869 Fragments arranged by size: 2869 2392 239 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5797 BstYI r'GATC_y Cuts at: 1972 3127 3494 3879 3896 5348 5434 5446 5792 Size: 1155 367 385 17 1452 86 12 346 Cuts at: 5792 1972 Size: 1977 Fragments arranged by size: 1977 1452 1155 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2992 513 Size: 552 1927 3318 Fragments arranged by size: 3318 1927 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2937 3006 3313 4107 4127 2937 Size: 69 307 794 20 4607 Fragments arranged by size: 4607 794 307 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2498 3244 4133 Size: 54 1425 50 275 18 668 746 889 Cuts at: 4133 5762 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 746 668 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 2337 2538 2685 3727 1353 Size: 873 111 201 147 1042 3423 Fragments arranged by size: 3423 1042 873 201 147 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2585 3860 4457 5244 5512 1239 Size: 393 953 1275 597 787 268 1524 Fragments arranged by size: 1524 1275 953 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2483 2484 2681 2911 3023 3063 2483 Size: 1 197 230 112 40 5217 Fragments arranged by size: 5217 230 197 112 40 1 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2251 4944 1678 Size: 573 2693 2531 Fragments arranged by size: 2693 2531 573 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2366 2398 2878 Size: 32 135 32 623 32 139 32 480 Cuts at: 2878 2910 4438 4470 4512 4544 1373 Size: 32 1528 32 42 32 2626 Fragments arranged by size: 2626 1528 623 480 139 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 2701 2770 3141 3387 669 Size: 988 281 370 393 69 371 246 3079 Fragments arranged by size: 3079 988 393 371 370 281 246 69 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2372 4911 5095 1305 Size: 1067 2539 184 2007 Fragments arranged by size: 2539 2007 1067 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3975 Fragments arranged by size: 3975 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3755 4114 4273 518 Size: 648 2589 359 159 2042 Fragments arranged by size: 2589 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 2417 3213 3890 5038 5283 1606 Size: 436 375 796 677 1148 245 2120 Fragments arranged by size: 2120 1148 796 677 436 375 245 NcoI C'CATG_G Cuts at: 513 1065 2992 513 Size: 552 1927 3318 Fragments arranged by size: 3318 1927 552 NdeI CA'TA_TG Cuts at: 387 2572 387 Size: 2185 3612 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 3155 4700 1132 Size: 97 1926 1545 2229 Fragments arranged by size: 2229 1926 1545 97 PasI CC'CwG_GG Cuts at: 1348 3019 1348 Size: 1671 4126 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2669 1108 Size: 1561 4236 PfoI T'CCnGG_A Cuts at: 2523 2523 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2819 2851 3866 3898 5379 5411 2819 Size: 32 1015 32 1481 32 3205 Fragments arranged by size: 3205 1481 1015 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2911 2911 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2483 2630 3022 3062 2483 Size: 147 392 40 5218 Fragments arranged by size: 5218 392 147 40 PspXI vC'TCGA_Gb Cuts at: 2582 2943 2582 Size: 361 5436 PstI C_TGCA'G Cuts at: 830 3149 830 Size: 2319 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2417 2417 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SanDI GG'GwC_CC Cuts at: 2911 2911 Size: 5797 SapI GCTCTTCn'nnn_ Cuts at: 2226 2538 2226 Size: 312 5485 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2412 3145 4280 4961 5152 826 Size: 219 1367 733 1135 681 191 1471 Fragments arranged by size: 1471 1367 1135 733 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 2629 3124 1872 Size: 757 495 4545 Fragments arranged by size: 4545 757 495 SmlI C'TyrA_G Cuts at: 820 1017 1943 2282 2290 2582 2943 3901 4802 Size: 197 926 339 8 292 361 958 901 Cuts at: 4802 5064 820 Size: 262 1553 Fragments arranged by size: 1553 958 926 901 361 339 292 262 197 8 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 2267 2296 1517 Size: 750 29 5018 Fragments arranged by size: 5018 750 29 StyI C'CwwG_G Cuts at: 513 1065 2262 2685 2842 2992 3236 513 Size: 552 1197 423 157 150 244 3074 Fragments arranged by size: 3074 1197 552 423 244 157 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3824 3841 3994 4179 4624 325 Size: 1255 2244 17 153 185 445 1498 Fragments arranged by size: 2244 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2979 4043 5755 Size: 274 80 33 51 1443 1002 1064 1712 Cuts at: 5755 96 Size: 138 Fragments arranged by size: 1712 1443 1064 1002 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2102 2102 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 2582 2943 1943 Size: 639 361 4797 Fragments arranged by size: 4797 639 361 XmaI C'CCGG_G Cuts at: 1870 2627 3122 1870 Size: 757 495 4545 Fragments arranged by size: 4545 757 495 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI