(Circular) (Six-base) MAPSORT of: pFN21AB7628.seq Check: 3331 from: 1 to: 5824 pFN21AB7628 5824 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2347 2347 Size: 5824 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 2068 3145 2068 Size: 1077 4747 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2523 3887 5271 5539 1632 Size: 891 1364 1384 268 1917 Fragments arranged by size: 1917 1384 1364 891 268 AfeI AGC'GCT Cuts at: 2282 2282 Size: 5824 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 2552 4553 2552 Size: 2001 3823 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5824 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2137 2747 3016 3149 1870 Size: 73 120 74 610 269 133 4545 Fragments arranged by size: 4545 610 269 133 120 74 73 BamHI G'GATC_C Cuts at: 2753 3154 3521 2753 Size: 401 367 5056 Fragments arranged by size: 5056 401 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2195 2495 3145 4600 Size: 325 217 627 281 127 300 650 1455 Cuts at: 4600 618 Size: 1842 Fragments arranged by size: 1842 1455 650 627 325 300 281 217 127 BanII G_rGCy'C Cuts at: 1391 2861 3084 3143 1391 Size: 1470 223 59 4072 Fragments arranged by size: 4072 1470 223 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4853 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2308 2342 4015 4049 1903 Size: 34 50 34 287 34 1673 34 3678 Fragments arranged by size: 3678 1673 287 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BfrBI ATG'CAT Cuts at: 2875 2875 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2778 4435 136 Size: 107 122 71 631 605 1106 1657 1525 Fragments arranged by size: 1657 1525 1106 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2919 3885 5041 1790 Size: 1129 966 1156 2573 Fragments arranged by size: 2573 1156 1129 966 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2418 2857 2914 Size: 416 53 83 399 24 1001 439 57 Cuts at: 2914 3555 4513 442 Size: 641 958 1753 Fragments arranged by size: 1753 1001 958 641 439 416 399 83 57 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3042 3042 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4484 1239 Size: 3245 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2252 3949 4814 5112 2252 Size: 1697 865 298 2964 Fragments arranged by size: 2964 1697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2183 3631 4013 4648 Size: 53 83 186 936 650 1448 382 635 Cuts at: 4648 275 Size: 1451 Fragments arranged by size: 1451 1448 936 650 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3085 3115 1178 Size: 30 165 30 1682 30 3887 Fragments arranged by size: 3887 1682 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2175 2402 3128 1186 Size: 989 227 726 3882 Fragments arranged by size: 3882 989 726 227 BseYI C'CCAG_C Cuts at: 2984 3083 5027 5523 2984 Size: 99 1944 496 3285 Fragments arranged by size: 3285 1944 496 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2509 1163 Size: 573 773 4478 Fragments arranged by size: 4478 773 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2433 3274 4035 5063 1540 Size: 275 186 432 841 761 1028 2301 Fragments arranged by size: 2301 1028 841 761 432 275 186 BsiHKAI G_wGCw'C Cuts at: 2861 3047 3885 3970 5041 2861 Size: 186 838 85 1071 3644 Fragments arranged by size: 3644 1071 838 186 85 BsmI GAATG_Cn' Cuts at: 2021 2875 3335 3428 2021 Size: 854 460 93 4417 Fragments arranged by size: 4417 854 460 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2658 2658 Size: 5824 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2861 2919 3047 3084 3143 3885 3970 Size: 399 1071 58 128 37 59 742 85 Cuts at: 3970 5041 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1071 742 399 128 85 59 58 37 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 2478 3713 5443 2478 Size: 1235 1730 2859 Fragments arranged by size: 2859 1730 1235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2347 3179 844 Size: 1503 832 3489 Fragments arranged by size: 3489 1503 832 BsrBI CCG'CTC Cuts at: 1394 1823 2266 3711 1394 Size: 429 443 1445 3507 Fragments arranged by size: 3507 1445 443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2859 4319 4493 65 Size: 1183 1611 1460 174 1396 Fragments arranged by size: 1611 1460 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2512 4468 4655 1700 Size: 110 702 1956 187 2869 Fragments arranged by size: 2869 1956 702 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 2316 794 Size: 1522 4302 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2707 2707 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2753 3154 3521 3906 3923 5375 5461 5473 Size: 781 401 367 385 17 1452 86 12 Cuts at: 5473 5819 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 781 401 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2216 2248 2700 513 Size: 552 1151 32 452 3637 Fragments arranged by size: 3637 1151 552 452 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5085 Fragments arranged by size: 5085 683 56 BtsI GCAGTG_nn' Cuts at: 2417 2497 2529 3060 3340 4134 4154 2417 Size: 80 32 531 280 794 20 4087 Fragments arranged by size: 4087 794 531 280 80 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2349 2430 Size: 54 1425 50 275 18 297 222 81 Cuts at: 2430 2514 3271 4160 5789 8 Size: 84 757 889 1629 43 Fragments arranged by size: 1629 1425 889 757 297 275 222 84 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2430 3271 1537 Size: 275 618 841 4090 Fragments arranged by size: 4090 841 618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3754 1353 Size: 2401 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2859 2859 Size: 5824 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2523 3887 4484 5271 5539 1239 Size: 393 891 1364 597 787 268 1524 Fragments arranged by size: 1524 1364 891 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2779 2779 Size: 5824 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FspI TGC'GCA Cuts at: 2233 4330 2233 Size: 2097 3727 FspAI rTGC'GCAy Cuts at: 2233 2233 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2117 2145 2284 4971 1678 Size: 439 28 139 2687 2531 Fragments arranged by size: 2687 2531 439 139 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2538 2570 2930 2962 3059 Size: 32 135 32 966 32 360 32 97 Cuts at: 3059 3091 4465 4497 4539 4571 1373 Size: 32 1374 32 42 32 2626 Fragments arranged by size: 2626 1374 966 360 135 97 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 2072 3149 2072 Size: 1077 4747 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 2166 3057 4938 5122 1305 Size: 720 141 891 1881 184 2007 Fragments arranged by size: 2007 1881 891 720 184 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4002 Fragments arranged by size: 4002 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3782 4141 4300 518 Size: 648 2616 359 159 2042 Fragments arranged by size: 2616 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2133 2218 3240 3917 5065 5310 1606 Size: 527 85 1022 677 1148 245 2120 Fragments arranged by size: 2120 1148 1022 677 527 245 85 NaeI GCC'GGC Cuts at: 2514 2514 Size: 5824 NcoI C'CATG_G Cuts at: 513 1065 2700 513 Size: 552 1635 3637 Fragments arranged by size: 3637 1635 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2512 2512 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NsiI A_TGCA'T Cuts at: 2877 2877 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2293 2594 3182 4727 1132 Size: 97 1064 301 588 1545 2229 Fragments arranged by size: 2229 1545 1064 588 301 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PfoI T'CCnGG_A Cuts at: 2559 2559 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspXI vC'TCGA_Gb Cuts at: 2137 2137 Size: 5824 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2237 2269 2237 Size: 32 5792 PstI C_TGCA'G Cuts at: 830 2243 2536 3176 830 Size: 1413 293 640 3478 Fragments arranged by size: 3478 1413 640 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 SacI G_AGCT'C Cuts at: 2861 2861 Size: 5824 SacII CC_GC'GG Cuts at: 2219 2219 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 2696 4072 5784 2696 Size: 1376 1712 2736 Fragments arranged by size: 2736 1712 1376 SexAI A'CCwGG_T Cuts at: 2610 2610 Size: 5824 SfcI C'TryA_G Cuts at: 826 1045 2239 2532 2632 3172 4307 4988 5179 Size: 219 1194 293 100 540 1135 681 191 Cuts at: 5179 826 Size: 1471 Fragments arranged by size: 1471 1194 1135 681 540 293 219 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2065 3151 1872 Size: 193 1086 4545 Fragments arranged by size: 4545 1086 193 SmlI C'TyrA_G Cuts at: 820 1017 1943 2137 2267 3016 3928 4829 5091 Size: 197 926 194 130 749 912 901 262 Cuts at: 5091 820 Size: 1553 Fragments arranged by size: 1553 926 912 901 749 262 197 194 130 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2700 2706 3263 513 Size: 552 1635 6 557 3074 Fragments arranged by size: 3074 1635 557 552 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2183 3851 3868 4021 4206 4651 325 Size: 1255 603 1668 17 153 185 445 1498 Fragments arranged by size: 1668 1498 1255 603 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2694 2865 4070 Size: 274 80 33 51 1443 717 171 1205 Cuts at: 4070 5782 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1205 717 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2745 2777 2781 2813 3085 3117 5784 5816 2745 Size: 32 4 32 272 32 2667 32 2753 Fragments arranged by size: 2753 2667 272 32 32 32 32 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 2137 3016 1943 Size: 194 879 4751 Fragments arranged by size: 4751 879 194 XmaI C'CCGG_G Cuts at: 1870 2063 3149 1870 Size: 193 1086 4545 Fragments arranged by size: 4545 1086 193 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI KasI NarI NruI PacI PmlI PpuMI PspOMI PvuII RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI