(Circular) (Six-base) MAPSORT of: pFN21AB7623.seq Check: 5942 from: 1 to: 5647 pFN21AB7623 5647 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2823 2990 1937 Size: 886 167 4594 Fragments arranged by size: 4594 886 167 Acc65I G'GTAC_C Cuts at: 2968 2968 Size: 5647 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3710 5094 5362 1632 Size: 2078 1384 268 1917 Fragments arranged by size: 2078 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 2523 2832 2928 4546 5523 1203 Size: 1320 309 96 1618 977 1327 Fragments arranged by size: 1618 1327 1320 977 309 96 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5647 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5647 AlwNI CAG_nnn'CTG Cuts at: 2461 3390 4962 2461 Size: 929 1572 3146 Fragments arranged by size: 3146 1572 929 ApaI G_GGCC'C Cuts at: 2091 2205 2696 2912 2091 Size: 114 491 216 4826 Fragments arranged by size: 4826 491 216 114 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2132 2204 2722 2842 2917 2972 1870 Size: 73 189 72 518 120 75 55 4545 Fragments arranged by size: 4545 518 189 120 75 73 72 55 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2572 2699 2968 4423 618 Size: 325 217 627 785 127 269 1455 1842 Fragments arranged by size: 1842 1455 785 627 325 269 217 127 BanII G_rGCy'C Cuts at: 1391 2055 2091 2205 2624 2696 2912 2966 1391 Size: 664 36 114 419 72 216 54 4072 Fragments arranged by size: 4072 664 419 216 114 72 54 36 BbeI G_GCGC'C Cuts at: 2576 2703 2576 Size: 127 5520 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2050 2796 2907 Size: 107 122 71 631 605 378 746 111 Cuts at: 2907 4258 136 Size: 1351 1525 Fragments arranged by size: 1525 1351 746 631 605 378 122 111 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 3064 3064 Size: 5647 Bme1580I G_kGCm'C Cuts at: 1790 2091 2205 2696 2912 3708 4864 1790 Size: 301 114 491 216 796 1156 2573 Fragments arranged by size: 2573 1156 796 491 301 216 114 BmgBI CAC'GTC Cuts at: 1318 2524 1318 Size: 1206 4441 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2563 3378 4336 Size: 416 53 83 399 24 1146 815 958 Cuts at: 4336 442 Size: 1753 Fragments arranged by size: 1753 1146 958 815 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 4307 1239 Size: 815 2253 2579 Fragments arranged by size: 2579 2253 815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2849 3772 4637 4935 2849 Size: 923 865 298 3561 Fragments arranged by size: 3561 923 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4310 882 Size: 617 2811 2219 Fragments arranged by size: 2811 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2485 2835 3438 493 Size: 711 1281 350 603 2702 Fragments arranged by size: 2702 1281 711 603 350 BsaBI GATnn'nnATC Cuts at: 5641 5641 Size: 5647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2573 2700 3454 Size: 53 83 186 936 477 563 127 754 Cuts at: 3454 3836 4471 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 754 635 563 477 382 186 127 83 53 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5422 Fragments arranged by size: 5422 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 2656 2746 1186 Size: 1192 278 90 4087 Fragments arranged by size: 4087 1192 278 90 BseYI C'CCAG_C Cuts at: 2251 2654 4850 5346 2251 Size: 403 2196 496 2552 Fragments arranged by size: 2552 2196 496 403 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 1163 Size: 573 792 4282 Fragments arranged by size: 4282 792 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2060 3097 3858 4886 1540 Size: 275 186 59 1037 761 1028 2301 Fragments arranged by size: 2301 1037 1028 761 275 186 59 BsiHKAI G_wGCw'C Cuts at: 3708 3793 4864 3708 Size: 85 1071 4491 Fragments arranged by size: 4491 1071 85 BsiWI C'GTAC_G Cuts at: 2282 2793 2282 Size: 511 5136 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2091 2205 2624 2696 2912 2966 Size: 399 265 36 114 419 72 216 54 Cuts at: 2966 3708 3793 4864 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 419 399 265 216 114 85 72 54 36 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3002 844 Size: 2158 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2038 2131 2584 3534 1394 Size: 429 215 93 453 950 3507 Fragments arranged by size: 3507 950 453 429 215 93 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2534 2750 4291 4478 1700 Size: 110 724 216 1541 187 2869 Fragments arranged by size: 2869 1541 724 216 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 2232 794 Size: 1438 4209 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5647 BstXI CCAn_nnnn'nTGG Cuts at: 2569 2569 Size: 5647 BstYI r'GATC_y Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642 Size: 1005 367 385 17 1452 86 12 346 Cuts at: 5642 1972 Size: 1977 Fragments arranged by size: 1977 1452 1005 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 3163 3957 3977 3163 Size: 794 20 4833 Fragments arranged by size: 4833 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2680 4654 809 Size: 507 1364 1974 1802 Fragments arranged by size: 1974 1802 1364 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2057 2769 3094 Size: 54 1425 50 275 18 227 712 325 Cuts at: 3094 3983 5612 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 712 325 275 227 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2057 3094 1537 Size: 275 245 1037 4090 Fragments arranged by size: 4090 1037 275 245 EarI CTCTTCn'nnn_ Cuts at: 1353 3577 1353 Size: 2224 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2131 4234 4608 4754 387 Size: 1064 138 542 2103 374 146 1280 Fragments arranged by size: 2103 1280 1064 542 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 3710 4307 5094 5362 1239 Size: 393 422 1656 597 787 268 1524 Fragments arranged by size: 1656 1524 787 597 422 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2471 2471 Size: 5647 EcoO109I rG'GnC_Cy Cuts at: 2087 2201 2908 2087 Size: 114 707 4826 Fragments arranged by size: 4826 707 114 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2576 2582 2703 2787 4794 1678 Size: 898 6 121 84 2007 2531 Fragments arranged by size: 2531 2007 898 121 84 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4288 4320 4362 4394 1373 Size: 32 135 32 2716 32 42 32 2626 Fragments arranged by size: 2716 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KasI G'GCGC_C Cuts at: 2572 2699 2572 Size: 127 5520 KpnI G_GTAC'C Cuts at: 2972 2972 Size: 5647 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 2928 5523 2928 Size: 2595 3052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3825 Fragments arranged by size: 3825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2354 2675 3605 3964 4123 518 Size: 648 1188 321 930 359 159 2042 Fragments arranged by size: 2042 1188 930 648 359 321 159 MspA1I CmG'CkG Cuts at: 1606 2272 2461 2499 2626 3063 3740 4888 5133 Size: 666 189 38 127 437 677 1148 245 Cuts at: 5133 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 666 437 245 189 127 38 NaeI GCC'GGC Cuts at: 2536 2536 Size: 5647 NarI GG'CG_CC Cuts at: 2573 2700 2573 Size: 127 5520 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NgoMIV G'CCGG_C Cuts at: 2534 2534 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 2716 3005 4550 1132 Size: 97 1487 289 1545 2229 Fragments arranged by size: 2229 1545 1487 289 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 2083 2461 1108 Size: 975 378 4294 Fragments arranged by size: 4294 975 378 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PmlI CAC'GTG Cuts at: 2835 2835 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2309 2341 3716 3748 5229 5261 2309 Size: 32 1375 32 1481 32 2695 Fragments arranged by size: 2695 1481 1375 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PspOMI G'GGCC_C Cuts at: 2087 2201 2692 2908 2087 Size: 114 491 216 4826 Fragments arranged by size: 4826 491 216 114 PspXI vC'TCGA_Gb Cuts at: 2842 2842 Size: 5647 PstI C_TGCA'G Cuts at: 830 2907 2999 830 Size: 2077 92 3478 Fragments arranged by size: 3478 2077 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 PvuII CAG'CTG Cuts at: 2461 2461 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SbfI CC_TGCA'GG Cuts at: 2907 2999 2907 Size: 92 5555 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2815 2903 2995 4130 4811 5002 826 Size: 219 1770 88 92 1135 681 191 1471 Fragments arranged by size: 1770 1471 1135 681 219 191 92 88 SfiI GGCCn_nnn'nGGCC Cuts at: 2907 2907 Size: 5647 SfoI GGC'GCC Cuts at: 2574 2701 2574 Size: 127 5520 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2974 1872 Size: 1102 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 2842 2864 3751 4652 4914 Size: 197 926 189 710 22 887 901 262 Cuts at: 4914 820 Size: 1553 Fragments arranged by size: 1553 926 901 887 710 262 197 189 22 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5647 SphI G_CATG'C Cuts at: 1132 2716 3005 1132 Size: 1584 289 3774 Fragments arranged by size: 3774 1584 289 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5647 StyI C'CwwG_G Cuts at: 513 1065 2004 2196 3086 513 Size: 552 939 192 890 3074 Fragments arranged by size: 3074 939 890 552 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2668 3674 3691 3844 4029 4474 325 Size: 1255 1088 1006 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1088 1006 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2937 3893 5605 Size: 274 80 33 51 1443 960 956 1712 Cuts at: 5605 96 Size: 138 Fragments arranged by size: 1712 1443 960 956 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5607 5639 5607 Size: 32 5615 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XhoI C'TCGA_G Cuts at: 1943 2132 2842 1943 Size: 189 710 4748 Fragments arranged by size: 4748 710 189 XmaI C'CCGG_G Cuts at: 1870 2972 1870 Size: 1102 4545 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PsrI RsrII SacI SacII SanDI SapI SexAI SrfI SwaI XcmI