Restriction map for
pFN21AB7621
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseRI BsgI BsiHKAI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BsrBI BsrDI BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MboI MfeI MflI MluI MluNI
MscI MslI MunI NdeI NdeII
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI Sau3AI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 638 TCCGGA T^CCGGA
ApaI 436 GGGCCC GGGCC^C
AvaI 180 CCCGGG C^[CT]CG[GA]G
BbrPI 130 CACGTG CAC^GTG
BbsI 855 GTCTTC GAAGAC(2/6)
BbvCI 808 CCTCAGC CCTCAGC(-5/-2)
BpuAI 855 GTCTTC GAAGAC(2/6)
BseAI 638 TCCGGA T^CCGGA
BsmAI 468 GTCTC GTCTC(1/5)
BsoBI 180 CCCGGG C^[CT]CG[GA]G
Bsp1407I 575 TGTACA T^GTACA
BspEI 638 TCCGGA T^CCGGA
BspMI 684 GCAGGT ACCTGC(4/8)
BsrGI 575 TGTACA T^GTACA
BssHII 170 GCGCGC G^CGCGC
BstEII 124 GGTTACC G^GT[ACGT]ACC
BstPI 124 GGTTACC G^GT[ACGT]ACC
BstXI 266 CCAACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 816 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 816 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 124 GGTTACC G^GT[ACGT]ACC
EcoT14I 311 CCATGG C^C[AT][AT]GG
HinfI 846 GAGTC G^A[ACGT]TC
KspI 254 CCGCGG CCGC^GG
MroI 638 TCCGGA T^CCGGA
MseI 689 TTAA T^TAA
NcoI 311 CCATGG C^CATGG
NheI 654 GCTAGC G^CTAGC
NspI 35 GCATGT [GA]CATG^[CT]
PflMI 588 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 846 GAGTC GAGTC(4/5)
PmaCI 130 CACGTG CAC^GTG
PmlI 130 CACGTG CAC^GTG
PpuMI 816 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 146 CTGCAG CTGCA^G
SacII 254 CCGCGG CCGC^GG
SfiI 185 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 180 CCCGGG CCC^GGG
SmlI 843 CTTGAG C^T[CT][GA]AG
SspBI 575 TGTACA T^GTACA
StyI 311 CCATGG C^C[AT][AT]GG
TaqI 567 TCGA T^CGA
Tru9I 689 TTAA T^TAA
Tsp45I 931 GTCAC ^GT[GC]AC
TspRI 872 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 567 TCGA T^CGA
Van91I 588 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 180 CCCGGG C^CCGGG
XmaI 180 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 589 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
872 CAGTGTCTG
AvaII 582 GGACC G^G[AT]CC
817 GGTCC
BfaI 44 CTAG C^TAG
655 CTAG
BglI 56 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
186 GCCATCCTGGC
BpmI 595 CTGGAG CTGGAG(16/14)
601 CTGGAG
BsaAI 4 TACGTG [CT]AC^GT[GA]
130 CACGTG
BsaWI 638 TCCGGA [AT]^CCGG[AT]
849 TCCGGT
BsrFI 195 GCCGGC [GA]^CCGG[CT]
513 GCCGGC
BsrI 227 ACTGG ACTGG(1/-1)
907 ACTGG
Cfr10I 195 GCCGGC [GA]^CCGG[CT]
513 GCCGGC
EcoNI 18 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
808 CCTCAGCCAGG
MaeI 44 CTAG C^TAG
655 CTAG
MaeIII 125 GTTAC ^GT[ACGT]AC
931 GTCAC
MboII 738 GAAGA GAAGA(8/7)
856 TCTTC
NaeI 195 GCCGGC GCC^GGC
513 GCCGGC
NgoMIV 195 GCCGGC G^CCGGC
513 GCCGGC
NlaIII 36 CATG CATG^
312 CATG
NotI 136 GCGGCCGC GC^GGCCGC
256 GCGGCCGC
PvuII 143 CAGCTG CAG^CTG
463 CAGCTG
SfaNI 504 GATGC GCATC(5/9)
357 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BanII BbeI BbvI BcnI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EaeI EagI EclXI
Eco52I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
MaeII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NlaIV
RsaI Sau96I ScrFI SfcI SfoI
TseI