(Circular) (Six-base) MAPSORT of: pFN21AB7621.seq Check: 2216 from: 1 to: 5644 pFN21AB7621 5644 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3707 5091 5359 1632 Size: 2075 1384 268 1917 Fragments arranged by size: 2075 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 4543 5520 1203 Size: 3340 977 1327 Fragments arranged by size: 3340 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5644 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 2599 2882 3387 4959 2599 Size: 283 505 1572 3284 Fragments arranged by size: 3284 1572 505 283 ApaI G_GGCC'C Cuts at: 2445 2445 Size: 5644 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 4198 160 Size: 872 3166 1606 Fragments arranged by size: 3166 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2185 2969 1870 Size: 73 242 784 4545 Fragments arranged by size: 4545 784 242 73 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2213 2255 2965 4420 Size: 325 217 627 296 130 42 710 1455 Cuts at: 4420 618 Size: 1842 Fragments arranged by size: 1842 1455 710 627 325 296 217 130 42 BanII G_rGCy'C Cuts at: 1391 2445 2679 2847 2963 1391 Size: 1054 234 168 116 4072 Fragments arranged by size: 4072 1054 234 168 116 BbeI G_GCGC'C Cuts at: 2087 2217 2259 2087 Size: 130 42 5472 Fragments arranged by size: 5472 130 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2853 928 Size: 971 954 3719 Fragments arranged by size: 3719 971 954 BbvCI CC'TCA_GC Cuts at: 2814 2814 Size: 5644 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2067 2197 4255 Size: 107 122 71 631 605 395 130 2058 Cuts at: 4255 136 Size: 1525 Fragments arranged by size: 2058 1525 631 605 395 130 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2445 3705 4861 1790 Size: 655 1260 1156 2573 Fragments arranged by size: 2573 1260 1156 655 BmgBI CAC'GTC Cuts at: 1318 2035 1318 Size: 717 4927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2922 3375 4333 Size: 416 53 83 399 24 1505 453 958 Cuts at: 4333 442 Size: 1753 Fragments arranged by size: 1753 1505 958 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2663 1056 Size: 1607 4037 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2621 2627 4304 1239 Size: 1382 6 1677 2579 Fragments arranged by size: 2579 1677 1382 6 Bpu10I CC'TnA_GC Cuts at: 2814 2814 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2869 3769 4634 4932 2869 Size: 900 865 298 3581 Fragments arranged by size: 3581 900 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2011 2137 3435 493 Size: 711 807 126 1298 2702 Fragments arranged by size: 2702 1298 807 711 126 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 2214 2256 3451 Size: 53 83 186 936 551 130 42 1195 Cuts at: 3451 3833 4468 275 Size: 382 635 1451 Fragments arranged by size: 1451 1195 936 635 551 382 186 130 83 53 42 BsaWI w'CCGG_w Cuts at: 2643 2854 4077 4475 4749 4896 5307 2643 Size: 211 1223 398 274 147 411 2980 Fragments arranged by size: 2980 1223 411 398 274 211 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 2285 2315 2600 Size: 30 165 30 633 30 219 30 285 Cuts at: 2600 2630 1178 Size: 30 4192 Fragments arranged by size: 4192 633 285 219 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5644 BseYI C'CCAG_C Cuts at: 2671 2808 4847 5343 2671 Size: 137 2039 496 2972 Fragments arranged by size: 2972 2039 496 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2145 2265 2310 2385 3094 3855 Size: 275 186 144 120 45 75 709 761 Cuts at: 3855 4883 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 709 275 186 144 120 75 45 BsiHKAI G_wGCw'C Cuts at: 3705 3790 4861 3705 Size: 85 1071 4488 Fragments arranged by size: 4488 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2445 2679 2847 2963 3705 3790 4861 Size: 399 655 234 168 116 742 85 1071 Cuts at: 4861 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 655 399 234 168 116 85 BspEI T'CCGG_A Cuts at: 2643 5307 2643 Size: 2664 2980 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2680 2999 844 Size: 1836 319 3489 Fragments arranged by size: 3489 1836 319 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2200 2518 4288 4475 1700 Size: 110 390 318 1770 187 2869 Fragments arranged by size: 2869 1770 390 318 187 110 BsrGI T'GTAC_A Cuts at: 96 2580 96 Size: 2484 3160 BssHII G'CGCG_C Cuts at: 1925 2175 1925 Size: 250 5394 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 2129 1184 Size: 945 4699 BstXI CCAn_nnnn'nTGG Cuts at: 2278 2278 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2974 3341 3726 3743 5195 5281 5293 5639 Size: 1002 367 385 17 1452 86 12 346 Cuts at: 5639 1972 Size: 1977 Fragments arranged by size: 1977 1452 1002 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2259 2277 2316 513 Size: 552 1194 18 39 3841 Fragments arranged by size: 3841 1194 552 39 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2198 2262 Size: 54 1425 50 275 18 312 56 64 Cuts at: 2262 2307 2382 2482 3091 3980 5609 8 Size: 45 75 100 609 889 1629 43 Fragments arranged by size: 1629 1425 889 609 312 275 100 75 64 56 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2142 2262 2307 2382 3091 1537 Size: 275 330 120 45 75 709 4090 Fragments arranged by size: 4090 709 330 275 120 75 45 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2506 2778 4231 4605 4751 387 Size: 1064 138 917 272 1453 374 146 1280 Fragments arranged by size: 1453 1280 1064 917 374 272 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2621 2627 3707 4304 5091 5359 1239 Size: 393 989 6 1080 597 787 268 1524 Fragments arranged by size: 1524 1080 989 787 597 393 268 6 EcoNI CCTnn'n_nnAGG Cuts at: 2027 2817 2027 Size: 790 4854 EcoO109I rG'GnC_Cy Cuts at: 2822 2822 Size: 5644 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2087 2217 2259 4791 1678 Size: 409 130 42 2532 2531 Fragments arranged by size: 2532 2531 409 130 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2456 2488 4285 4317 4359 Size: 32 135 32 884 32 1797 32 42 Cuts at: 4359 4391 1373 Size: 32 2626 Fragments arranged by size: 2626 1797 884 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KasI G'GCGC_C Cuts at: 2083 2213 2255 2083 Size: 130 42 5472 Fragments arranged by size: 5472 130 42 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3822 Fragments arranged by size: 3822 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3602 3961 4120 518 Size: 648 843 1593 359 159 2042 Fragments arranged by size: 2042 1593 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2150 2156 2261 2456 2470 2488 2596 3060 Size: 544 6 105 195 14 18 108 464 Cuts at: 3060 3737 4885 5130 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 544 464 245 195 108 105 18 14 6 NaeI GCC'GGC Cuts at: 2202 2520 2202 Size: 318 5326 NarI GG'CG_CC Cuts at: 2084 2214 2256 2084 Size: 130 42 5472 Fragments arranged by size: 5472 130 42 NcoI C'CATG_G Cuts at: 513 1065 2316 513 Size: 552 1251 3841 Fragments arranged by size: 3841 1251 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NgoMIV G'CCGG_C Cuts at: 2200 2518 2200 Size: 318 5326 NheI G'CTAG_C Cuts at: 1052 2659 1052 Size: 1607 4037 NotI GC'GGCC_GC Cuts at: 2142 2262 3091 2142 Size: 120 829 4695 Fragments arranged by size: 4695 829 120 NspI r_CATG'y Cuts at: 1132 1229 2044 3002 4547 1132 Size: 97 815 958 1545 2229 Fragments arranged by size: 2229 1545 958 815 97 PasI CC'CwG_GG Cuts at: 1348 2894 1348 Size: 1546 4098 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 2599 1108 Size: 1491 4153 PfoI T'CCnGG_A Cuts at: 2022 2223 2022 Size: 201 5443 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PmlI CAC'GTG Cuts at: 2137 2137 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2822 2822 Size: 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2441 2441 Size: 5644 PstI C_TGCA'G Cuts at: 830 2155 2996 830 Size: 1325 841 3478 Fragments arranged by size: 3478 1325 841 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2150 2470 2150 Size: 320 5324 SacII CC_GC'GG Cuts at: 2262 2262 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SfcI C'TryA_G Cuts at: 826 1045 2151 2323 2623 2992 4127 4808 4999 Size: 219 1106 172 300 369 1135 681 191 Cuts at: 4999 826 Size: 1471 Fragments arranged by size: 1471 1135 1106 681 369 300 219 191 172 SfiI GGCCn_nnn'nGGCC Cuts at: 2197 2197 Size: 5644 SfoI GGC'GCC Cuts at: 2085 2215 2257 2085 Size: 130 42 5472 Fragments arranged by size: 5472 130 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2187 2971 1872 Size: 315 784 4545 Fragments arranged by size: 4545 784 315 SmlI C'TyrA_G Cuts at: 820 1017 1943 2848 3748 4649 4911 820 Size: 197 926 905 900 901 262 1553 Fragments arranged by size: 1553 926 905 901 900 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2316 3083 513 Size: 552 1251 767 3074 Fragments arranged by size: 3074 1251 767 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2954 3671 3688 3841 4026 4471 325 Size: 1255 1374 717 17 153 185 445 1498 Fragments arranged by size: 1498 1374 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 3890 5602 Size: 274 80 33 51 1443 603 1310 1712 Cuts at: 5602 96 Size: 138 Fragments arranged by size: 1712 1443 1310 603 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2185 2969 1870 Size: 315 784 4545 Fragments arranged by size: 4545 784 315 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI