Restriction map for
pFN21AB7621
(Linear) (Six-base) MAPSORT of: KIBB7621.seq Check: 8038 from: 1 to: 939
KIBB7621.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 594 877 939
Size: 594 283 62
Fragments arranged by size:
594 283 62
ApaI G_GGCC'C
Cuts at: 0 440 939
Size: 440 499
AvaI C'yCGr_G
Cuts at: 0 180 939
Size: 180 759
BanI G'GyrC_C
Cuts at: 0 78 208 250 939
Size: 78 130 42 689
Fragments arranged by size:
689 130 78 42
BanII G_rGCy'C
Cuts at: 0 440 674 842 939
Size: 440 234 168 97
Fragments arranged by size:
440 234 168 97
BbeI G_GCGC'C
Cuts at: 0 82 212 254 939
Size: 82 130 42 685
Fragments arranged by size:
685 130 82 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 848 939
Size: 848 91
BbvCI CC'TCA_GC
Cuts at: 0 809 939
Size: 809 130
BglI GCCn_nnn'nGGC
Cuts at: 0 62 192 939
Size: 62 130 747
Fragments arranged by size:
747 130 62
Bme1580I G_kGCm'C
Cuts at: 0 440 939
Size: 440 499
BmgBI CAC'GTC
Cuts at: 0 30 939
Size: 30 909
BmrI ACTGGGnnnn_n'
Cuts at: 0 917 939
Size: 917 22
BmtI G_CTAG'C
Cuts at: 0 658 939
Size: 658 281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 622 939
Size: 616 6 317
Fragments arranged by size:
616 317 6
Bpu10I CC'TnA_GC
Cuts at: 0 809 939
Size: 809 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 864 939
Size: 864 75
BsaAI yAC'GTr
Cuts at: 0 6 132 939
Size: 6 126 807
Fragments arranged by size:
807 126 6
BsaHI Gr'CG_yC
Cuts at: 0 79 209 251 939
Size: 79 130 42 688
Fragments arranged by size:
688 130 79 42
BsaWI w'CCGG_w
Cuts at: 0 638 849 939
Size: 638 211 90
Fragments arranged by size:
638 211 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 280 310 595 625 939
Size: 31 30 219 30 285 30 314
Fragments arranged by size:
314 285 219 31 30 30 30
BseYI C'CCAG_C
Cuts at: 0 666 803 939
Size: 666 137 136
Fragments arranged by size:
666 137 136
BsiEI CG_ry'CG
Cuts at: 0 140 260 305 380 939
Size: 140 120 45 75 559
Fragments arranged by size:
559 140 120 75 45
Bsp1286I G_dGCh'C
Cuts at: 0 440 674 842 939
Size: 440 234 168 97
Fragments arranged by size:
440 234 168 97
BspEI T'CCGG_A
Cuts at: 0 638 939
Size: 638 301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 675 939
Size: 675 264
BsrFI r'CCGG_y
Cuts at: 0 195 513 939
Size: 195 318 426
Fragments arranged by size:
426 318 195
BsrGI T'GTAC_A
Cuts at: 0 575 939
Size: 575 364
BssHII G'CGCG_C
Cuts at: 0 170 939
Size: 170 769
BstEII G'GTnAC_C
Cuts at: 0 124 939
Size: 124 815
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 939
Size: 273 666
BtgI C'CryG_G
Cuts at: 0 254 272 311 939
Size: 254 18 39 628
Fragments arranged by size:
628 254 39 18
EaeI y'GGCC_r
Cuts at: 0 137 193 257 302 377 477 939
Size: 137 56 64 45 75 100 462
Fragments arranged by size:
462 137 100 75 64 56 45
EagI C'GGCC_G
Cuts at: 0 137 257 302 377 939
Size: 137 120 45 75 562
Fragments arranged by size:
562 137 120 75 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 501 773 939
Size: 501 272 166
Fragments arranged by size:
501 272 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 622 939
Size: 616 6 317
Fragments arranged by size:
616 317 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 812 939
Size: 22 790 127
Fragments arranged by size:
790 127 22
EcoO109I rG'GnC_Cy
Cuts at: 0 817 939
Size: 817 122
HaeII r_GCGC'y
Cuts at: 0 82 212 254 939
Size: 82 130 42 685
Fragments arranged by size:
685 130 82 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 939
Size: 451 32 456
Fragments arranged by size:
456 451 32
KasI G'GCGC_C
Cuts at: 0 78 208 250 939
Size: 78 130 42 689
Fragments arranged by size:
689 130 78 42
MspA1I CmG'CkG
Cuts at: 0 145 151 256 451 465 483 591 939
Size: 145 6 105 195 14 18 108 348
Fragments arranged by size:
348 195 145 108 105 18 14 6
NaeI GCC'GGC
Cuts at: 0 197 515 939
Size: 197 318 424
Fragments arranged by size:
424 318 197
NarI GG'CG_CC
Cuts at: 0 79 209 251 939
Size: 79 130 42 688
Fragments arranged by size:
688 130 79 42
NcoI C'CATG_G
Cuts at: 0 311 939
Size: 311 628
NgoMIV G'CCGG_C
Cuts at: 0 195 513 939
Size: 195 318 426
Fragments arranged by size:
426 318 195
NheI G'CTAG_C
Cuts at: 0 654 939
Size: 654 285
NotI GC'GGCC_GC
Cuts at: 0 137 257 939
Size: 137 120 682
Fragments arranged by size:
682 137 120
NspI r_CATG'y
Cuts at: 0 39 939
Size: 39 900
PasI CC'CwG_GG
Cuts at: 0 889 939
Size: 889 50
PflMI CCAn_nnn'nTGG
Cuts at: 0 594 939
Size: 594 345
PfoI T'CCnGG_A
Cuts at: 0 17 218 939
Size: 17 201 721
Fragments arranged by size:
721 201 17
PmlI CAC'GTG
Cuts at: 0 132 939
Size: 132 807
PpuMI rG'GwC_Cy
Cuts at: 0 817 939
Size: 817 122
PspOMI G'GGCC_C
Cuts at: 0 436 939
Size: 436 503
PstI C_TGCA'G
Cuts at: 0 150 939
Size: 150 789
PvuII CAG'CTG
Cuts at: 0 145 465 939
Size: 145 320 474
Fragments arranged by size:
474 320 145
SacII CC_GC'GG
Cuts at: 0 257 939
Size: 257 682
SfcI C'TryA_G
Cuts at: 0 146 318 618 939
Size: 146 172 300 321
Fragments arranged by size:
321 300 172 146
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 192 939
Size: 192 747
SfoI GGC'GCC
Cuts at: 0 80 210 252 939
Size: 80 130 42 687
Fragments arranged by size:
687 130 80 42
SmaI CCC'GGG
Cuts at: 0 182 939
Size: 182 757
SmlI C'TyrA_G
Cuts at: 0 843 939
Size: 843 96
StyI C'CwwG_G
Cuts at: 0 311 939
Size: 311 628
TatI w'GTAC_w
Cuts at: 0 575 939
Size: 575 364
XmaI C'CCGG_G
Cuts at: 0 180 939
Size: 180 759
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI
BglI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseYI BsiEI Bsp1286I BspEI
BspMI BsrFI BsrGI BssHII BstEII BstXI BtgI EaeI
EagI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacII SfcI SfiI SfoI SmaI SmlI StyI TatI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BglII BlpI BplI BsaI BsaBI BseRI
BsgI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrDI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NdeI NruI
NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmnI ZraI