Restriction map for
pFN21AB7620
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AhdI 843 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 989 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 843 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 78 GAGCAC G[AT]GC[AT]^C
AvaI 439 CCCGGG C^[CT]CG[GA]G
BbsI 1031 GTCTTC GAAGAC(2/6)
BfaI 740 CTAG C^TAG
BlpI 468 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 468 GCTCAGC GC^T[ACGT]AGC
BpuAI 1031 GTCTTC GAAGAC(2/6)
BsaWI 507 TCCGGT [AT]^CCGG[AT]
BsiHKAI 78 GAGCAC G[AT]GC[AT]^C
BsoBI 439 CCCGGG C^[CT]CG[GA]G
BsrBI 111 CCGCTC CCGCTC(-3/-3)
CelII 468 GCTCAGC GC^T[ACGT]AGC
EaeI 1071 TGGCCG [CT]^GGCC[GA]
Eam1105I 843 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MaeI 740 CTAG C^TAG
NspI 14 ACATGC [GA]CATG^[CT]
PstI 868 CTGCAG CTGCA^G
ScaI 699 AGTACT AGT^ACT
SmaI 439 CCCGGG CCC^GGG
SmlI 160 CTCAAG C^T[CT][GA]AG
StuI 815 AGGCCT AGG^CCT
TaqI 1035 TCGA T^CGA
TthHB8I 1035 TCGA T^CGA
XmaCI 439 CCCGGG C^CCGGG
XmaI 439 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 591 GTAC GT^AC
700 GTAC
ApaI 667 GGGCCC GGGCC^C
733 GGGCCC
BglI 247 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
726 GCCCCAAGGGC
BsaI 61 GAGACC GGTCTC(1/5)
68 GAGACC
BstXI 774 CCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1014 CCAGCCTGTTGG
BstYI 688 GGATCT [GA]^GATC[CT]
959 GGATCT
DsaI 141 CCATGG C^C[GA][CT]GG
408 CCATGG
FauI 128 CCCGC CCCGC(4/6)
749 CCCGC
HaeII 173 AGCGCC [GA]GCGC^[CT]
227 AGCGCC
MflI 688 GGATCT [GA]^GATC[CT]
959 GGATCT
MslI 205 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
973 CACCCCTGTG
NcoI 141 CCATGG C^CATGG
408 CCATGG
PleI 43 GACTC GAGTC(4/5)
504 GACTC
PpuMI 362 AGGTCCC [GA]G^G[AT]CC[CT]
527 GGGTCCC
RsaI 591 GTAC GT^AC
700 GTAC
SfcI 805 CTACAG C^T[GA][CT]AG
868 CTGCAG
TfiI 907 GATTC G^A[AT]TC
1010 GATTC
XhoII 688 GGATCT [GA]^GATC[CT]
959 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BssSI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI StyI TseI Tsp45I TspRI