Restriction map for
pFN21AB7620
(Circular) (Six-base) MAPSORT of: pFN21AB7620.seq Check: 7008 from: 1 to: 5806
pFN21AB7620 5806 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2693 2693
Size: 5806
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2326 2789 2910 3869 5253 5521 1632
Size: 694 463 121 959 1384 268 1917
Fragments arranged by size:
1917 1384 959 694 463 268 121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 2853 4535 2853
Size: 1682 4124
AleI CACnn'nnGTG
Cuts at: 1166 2214 2982 1166
Size: 1048 768 3990
Fragments arranged by size:
3990 1048 768
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 2999 3549 5121 2999
Size: 550 1572 3684
Fragments arranged by size:
3684 1572 550
ApaI G_GGCC'C
Cuts at: 2676 2742 2676
Size: 66 5740
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 3115 3341 3493 5602 1493
Size: 1622 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2444 3131 1870
Size: 73 501 687 4545
Fragments arranged by size:
4545 687 501 73
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 2515 2645 2789 3127
Size: 325 217 627 644 84 130 144 338
Cuts at: 3127 4582 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 644 627 338 325 217 144
130 84
BanII G_rGCy'C
Cuts at: 1391 2676 2733 2742 3125 1391
Size: 1285 57 9 383 4072
Fragments arranged by size:
4072 1285 383 57 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3029 928
Size: 971 1130 3705
Fragments arranged by size:
3705 1130 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2258 2737 4417
Size: 107 122 71 631 605 586 479 1680
Cuts at: 4417 136
Size: 1525
Fragments arranged by size:
1680 1525 631 605 586 479 122 107
71
BglII A'GATC_T
Cuts at: 5801 5801
Size: 5806
BlpI GC'TnA_GC
Cuts at: 2474 3223 2474
Size: 749 5057
Bme1580I G_kGCm'C
Cuts at: 1790 2676 2742 2949 3867 5023 1790
Size: 886 66 207 918 1156 2573
Fragments arranged by size:
2573 1156 918 886 207 66
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2007 2696 3537
Size: 416 53 83 399 24 590 689 841
Cuts at: 3537 4495 442
Size: 958 1753
Fragments arranged by size:
1753 958 841 689 590 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2538 2639 2745 2762 2847 3024 4466
Size: 1010 289 101 106 17 85 177 1442
Cuts at: 4466 1239
Size: 2579
Fragments arranged by size:
2579 1442 1010 289 177 106 101 85
17
Bpu10I CC'TnA_GC
Cuts at: 3101 3101
Size: 5806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 3931 4796 5094 2150
Size: 1781 865 298 2862
Fragments arranged by size:
2862 1781 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 2067 4469 882
Size: 617 561 7 2402 2219
Fragments arranged by size:
2402 2219 617 561 7
BsaAI yAC'GTr
Cuts at: 493 1204 3597 493
Size: 711 2393 2702
Fragments arranged by size:
2702 2393 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3613 3995 4630 275
Size: 53 83 186 936 2080 382 635 1451
Fragments arranged by size:
2080 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2512 4239 4637 4911 5058 5469 2512
Size: 1727 398 274 147 411 2849
Fragments arranged by size:
2849 1727 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2320 2706 2916 1186
Size: 1134 386 210 4076
Fragments arranged by size:
4076 1134 386 210
BseYI C'CCAG_C
Cuts at: 2397 3044 3049 5009 5505 2397
Size: 647 5 1960 496 2698
Fragments arranged by size:
2698 1960 647 496 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3256 4017 5045 1540
Size: 275 186 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2087 3867 3952 5023 2087
Size: 1780 85 1071 2870
Fragments arranged by size:
2870 1780 1071 85
BsmI GAATG_Cn'
Cuts at: 3317 3410 3317
Size: 93 5713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2676 2733 2742 2949 3125 3867
Size: 399 297 589 57 9 207 176 742
Cuts at: 3867 3952 5023 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 589 399 297 207 176
85 57 9
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2135 2614 2693 3161 844
Size: 1291 479 79 468 3489
Fragments arranged by size:
3489 1291 479 468 79
BsrBI CCG'CTC
Cuts at: 1394 1823 2118 3693 1394
Size: 429 295 1575 3507
Fragments arranged by size:
3507 1575 429 295
BsrDI GCAATG_nn'
Cuts at: 65 1248 4301 4475 65
Size: 1183 3053 174 1396
Fragments arranged by size:
3053 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4450 4637 1700
Size: 110 2640 187 2869
Fragments arranged by size:
2869 2640 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5806
BssSI C'ACGA_G
Cuts at: 2833 2897 2928 3866 4878 2833
Size: 64 31 938 1012 3761
Fragments arranged by size:
3761 1012 938 64 31
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5806
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstXI CCAn_nnnn'nTGG
Cuts at: 2786 3026 2786
Size: 240 5566
BstYI r'GATC_y
Cuts at: 1972 2693 2964 3136 3503 3888 3905 5357 5443
Size: 721 271 172 367 385 17 1452 86
Cuts at: 5443 5455 5801 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 721 385 367 346 271 172
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2146 2413 513
Size: 552 939 142 267 3906
Fragments arranged by size:
3906 939 552 267 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 2035 3322 4116 4136 2035
Size: 1287 794 20 3705
Fragments arranged by size:
3705 1287 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3076 3253 4142
Size: 54 1425 50 275 18 1246 177 889
Cuts at: 4142 5771 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1246 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3253 1537
Size: 275 1441 4090
Fragments arranged by size:
4090 1441 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 2808 3004 3736 1353
Size: 842 613 196 732 3423
Fragments arranged by size:
3423 842 732 613 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4393 4767 4913 387
Size: 1064 138 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2326 2538 2639 2745 2762 2789
Size: 393 617 77 212 101 106 17 27
Cuts at: 2789 2847 2910 3024 3869 4466 5253 5521 1239
Size: 58 63 114 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 617 597 393 268 212
114 106 101 77 63 58 27 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2266 3003 2192
Size: 74 737 4995
Fragments arranged by size:
4995 737 74
EcoO109I rG'GnC_Cy
Cuts at: 2368 2533 2673 2738 2739 2368
Size: 165 140 65 1 5435
Fragments arranged by size:
5435 165 140 65 1
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2182 2236 4953 1678
Size: 504 54 2717 2531
Fragments arranged by size:
2717 2531 504 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2701 2733 2839 2871 4447
Size: 32 135 32 1129 32 106 32 1576
Cuts at: 4447 4479 4521 4553 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1576 1129 135 106 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2147 4920 5104 1305
Size: 842 2773 184 2007
Fragments arranged by size:
2773 2007 842 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2214 2982 3764 4123 4282 518
Size: 648 1048 768 782 359 159 2042
Fragments arranged by size:
2042 1048 782 768 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2136 2659 2872 2888 3222 3899 5047 5292
Size: 530 523 213 16 334 677 1148 245
Cuts at: 5292 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 530 523 334 245 213
16
NcoI C'CATG_G
Cuts at: 513 1065 2004 2146 2413 513
Size: 552 939 142 267 3906
Fragments arranged by size:
3906 939 552 267 142
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 3253 3253
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2023 3164 4709 1132
Size: 97 794 1141 1545 2229
Fragments arranged by size:
2229 1545 1141 794 97
PasI CC'CwG_GG
Cuts at: 1348 2058 1348
Size: 710 5096
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5806
PfoI T'CCnGG_A
Cuts at: 2226 2504 2226
Size: 278 5528
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2368 2533 2368
Size: 165 5641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PspOMI G'GGCC_C
Cuts at: 2672 2738 2672
Size: 66 5740
PstI C_TGCA'G
Cuts at: 830 2877 3158 830
Size: 2047 281 3478
Fragments arranged by size:
3478 2047 281
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 2659 2872 2888 2659
Size: 213 16 5577
Fragments arranged by size:
5577 213 16
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SanDI GG'GwC_CC
Cuts at: 2533 2533
Size: 5806
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 2706 4054 5766 2706
Size: 1348 1712 2746
Fragments arranged by size:
2746 1712 1348
SfcI C'TryA_G
Cuts at: 826 1045 2810 2873 3154 4289 4970 5161 826
Size: 219 1765 63 281 1135 681 191 1471
Fragments arranged by size:
1765 1471 1135 681 281 219 191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5806
SmaI CCC'GGG
Cuts at: 1872 2446 3133 1872
Size: 574 687 4545
Fragments arranged by size:
4545 687 574
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 3910 4811 5073 820
Size: 197 926 222 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 2822 1517
Size: 1305 4501
StyI C'CwwG_G
Cuts at: 513 1065 2004 2146 2413 2528 2734 3245 513
Size: 552 939 142 267 115 206 511 3074
Fragments arranged by size:
3074 939 552 511 267 206 142 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2715 3833 3850 4003 4188 4633 325
Size: 1255 1135 1118 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1135 1118 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2704 4052 5764
Size: 274 80 33 51 1443 727 1348 1712
Cuts at: 5764 96
Size: 138
Fragments arranged by size:
1712 1443 1348 727 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5766 5798 5766
Size: 32 5774
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2444 3131 1870
Size: 574 687 4545
Fragments arranged by size:
4545 687 574
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI