Restriction map for
pFN21AB7620
(Linear) (Six-base) MAPSORT of: KIBB7620.seq Check: 8815 from: 1 to: 1101
KIBB7620.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 688 1101
Size: 688 413
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 784 905 1101
Size: 321 463 121 196
Fragments arranged by size:
463 321 196 121
AhdI GACnn_n'nnGTC
Cuts at: 0 848 1101
Size: 848 253
AleI CACnn'nnGTG
Cuts at: 0 209 977 1101
Size: 209 768 124
Fragments arranged by size:
768 209 124
AlwNI CAG_nnn'CTG
Cuts at: 0 994 1101
Size: 994 107
ApaI G_GGCC'C
Cuts at: 0 671 737 1101
Size: 671 66 364
Fragments arranged by size:
671 364 66
AvaI C'yCGr_G
Cuts at: 0 439 1101
Size: 439 662
BanI G'GyrC_C
Cuts at: 0 426 510 640 784 1101
Size: 426 84 130 144 317
Fragments arranged by size:
426 317 144 130 84
BanII G_rGCy'C
Cuts at: 0 671 728 737 1101
Size: 671 57 9 364
Fragments arranged by size:
671 364 57 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1024 1101
Size: 1024 77
BglI GCCn_nnn'nGGC
Cuts at: 0 253 732 1101
Size: 253 479 369
Fragments arranged by size:
479 369 253
BlpI GC'TnA_GC
Cuts at: 0 469 1101
Size: 469 632
Bme1580I G_kGCm'C
Cuts at: 0 671 737 944 1101
Size: 671 66 207 157
Fragments arranged by size:
671 207 157 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 2 691 1101
Size: 2 689 410
Fragments arranged by size:
689 410 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 533 634 740 757 842 1019 1101
Size: 244 289 101 106 17 85 177 82
Fragments arranged by size:
289 244 177 106 101 85 82 17
Bpu10I CC'TnA_GC
Cuts at: 0 1096 1101
Size: 1096 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 1101
Size: 145 956
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 62 1101
Size: 55 7 1039
Fragments arranged by size:
1039 55 7
BsaWI w'CCGG_w
Cuts at: 0 507 1101
Size: 507 594
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 315 701 911 1101
Size: 315 386 210 190
Fragments arranged by size:
386 315 210 190
BseYI C'CCAG_C
Cuts at: 0 392 1039 1044 1101
Size: 392 647 5 57
Fragments arranged by size:
647 392 57 5
BsiHKAI G_wGCw'C
Cuts at: 0 82 1101
Size: 82 1019
Bsp1286I G_dGCh'C
Cuts at: 0 82 671 728 737 944 1101
Size: 82 589 57 9 207 157
Fragments arranged by size:
589 207 157 82 57 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 130 609 688 1101
Size: 130 479 79 413
Fragments arranged by size:
479 413 130 79
BsrBI CCG'CTC
Cuts at: 0 113 1101
Size: 113 988
BssSI C'ACGA_G
Cuts at: 0 828 892 923 1101
Size: 828 64 31 178
Fragments arranged by size:
828 178 64 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 781 1021 1101
Size: 781 240 80
Fragments arranged by size:
781 240 80
BstYI r'GATC_y
Cuts at: 0 688 959 1101
Size: 688 271 142
Fragments arranged by size:
688 271 142
BtgI C'CryG_G
Cuts at: 0 141 408 1101
Size: 141 267 693
Fragments arranged by size:
693 267 141
BtsI GCAGTG_nn'
Cuts at: 0 30 1101
Size: 30 1071
EaeI y'GGCC_r
Cuts at: 0 1071 1101
Size: 1071 30
EarI CTCTTCn'nnn_
Cuts at: 0 190 803 999 1101
Size: 190 613 196 102
Fragments arranged by size:
613 196 190 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 321 533 634 740 757 784 842
Size: 244 77 212 101 106 17 27 58
Cuts at: 842 905 1019 1101
Size: 63 114 82
Fragments arranged by size:
244 212 114 106 101 82 77 63
58 27 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 261 998 1101
Size: 187 74 737 103
Fragments arranged by size:
737 187 103 74
EcoO109I rG'GnC_Cy
Cuts at: 0 363 528 668 733 734 1101
Size: 363 165 140 65 1 367
Fragments arranged by size:
367 363 165 140 65 1
HaeII r_GCGC'y
Cuts at: 0 177 231 1101
Size: 177 54 870
Fragments arranged by size:
870 177 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 696 728 834 866 1101
Size: 696 32 106 32 235
Fragments arranged by size:
696 235 106 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1101
Size: 142 959
MslI CAynn'nnrTG
Cuts at: 0 209 977 1101
Size: 209 768 124
Fragments arranged by size:
768 209 124
MspA1I CmG'CkG
Cuts at: 0 131 654 867 883 1101
Size: 131 523 213 16 218
Fragments arranged by size:
523 218 213 131 16
NcoI C'CATG_G
Cuts at: 0 141 408 1101
Size: 141 267 693
Fragments arranged by size:
693 267 141
NspI r_CATG'y
Cuts at: 0 18 1101
Size: 18 1083
PasI CC'CwG_GG
Cuts at: 0 53 1101
Size: 53 1048
PfoI T'CCnGG_A
Cuts at: 0 221 499 1101
Size: 221 278 602
Fragments arranged by size:
602 278 221
PpuMI rG'GwC_Cy
Cuts at: 0 363 528 1101
Size: 363 165 573
Fragments arranged by size:
573 363 165
PspOMI G'GGCC_C
Cuts at: 0 667 733 1101
Size: 667 66 368
Fragments arranged by size:
667 368 66
PstI C_TGCA'G
Cuts at: 0 872 1101
Size: 872 229
PvuII CAG'CTG
Cuts at: 0 654 867 883 1101
Size: 654 213 16 218
Fragments arranged by size:
654 218 213 16
SanDI GG'GwC_CC
Cuts at: 0 528 1101
Size: 528 573
ScaI AGT'ACT
Cuts at: 0 701 1101
Size: 701 400
SfcI C'TryA_G
Cuts at: 0 805 868 1101
Size: 805 63 233
Fragments arranged by size:
805 233 63
SmaI CCC'GGG
Cuts at: 0 441 1101
Size: 441 660
SmlI C'TyrA_G
Cuts at: 0 160 1101
Size: 160 941
StuI AGG'CCT
Cuts at: 0 817 1101
Size: 817 284
StyI C'CwwG_G
Cuts at: 0 141 408 523 729 1101
Size: 141 267 115 206 372
Fragments arranged by size:
372 267 206 141 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 710 1101
Size: 710 391
TatI w'GTAC_w
Cuts at: 0 699 1101
Size: 699 402
XmaI C'CCGG_G
Cuts at: 0 439 1101
Size: 439 662
Enzymes that do cut:
AarI AcuI AhdI AleI AlwNI ApaI AvaI BanI
BanII BbsI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BssSI BstXI BstYI BtgI BtsI EaeI EarI
Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MslI MspA1I
NcoI NspI PasI PfoI PpuMI PspOMI PstI PvuII
SanDI ScaI SfcI SmaI SmlI StuI StyI TaqII
TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI