(Circular) (Six-base) MAPSORT of: pFN21AB7588.seq Check: 8501 from: 1 to: 5572 pFN21AB7588 5572 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2823 2823 Size: 5572 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2075 3382 278 Size: 53 83 186 936 539 1307 2468 Fragments arranged by size: 2468 1307 936 539 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 1937 Size: 978 4594 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 3635 5019 5287 1632 Size: 477 1526 1384 268 1917 Fragments arranged by size: 1917 1526 1384 477 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 4471 5448 1203 Size: 3268 977 1327 Fragments arranged by size: 3268 1327 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5572 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2555 2587 2759 2791 2555 Size: 32 172 32 5336 Fragments arranged by size: 5336 172 32 32 AlwNI CAG_nnn'CTG Cuts at: 3315 4887 3315 Size: 1572 4000 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2378 2897 1870 Size: 73 174 261 519 4545 Fragments arranged by size: 4545 519 261 174 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2773 2806 2773 Size: 33 5539 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2321 2893 4348 618 Size: 325 217 627 534 572 1455 1842 Fragments arranged by size: 1842 1455 627 572 534 325 217 BanII G_rGCy'C Cuts at: 1391 2167 2379 2682 2690 2831 2891 1391 Size: 776 212 303 8 141 60 4072 Fragments arranged by size: 4072 776 303 212 141 60 8 BbeI G_GCGC'C Cuts at: 2325 2325 Size: 5572 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2115 2483 928 Size: 971 216 368 4017 Fragments arranged by size: 4017 971 368 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 1903 Size: 34 1826 34 3678 Fragments arranged by size: 3678 1826 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3464 4674 3464 Size: 1210 4362 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2513 4183 136 Size: 107 122 71 631 605 841 1670 1525 Fragments arranged by size: 1670 1525 841 631 605 122 107 71 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2702 2989 2702 Size: 287 5285 Bme1580I G_kGCm'C Cuts at: 1790 3633 4789 1790 Size: 1843 1156 2573 Fragments arranged by size: 2573 1843 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4261 442 Size: 416 53 83 399 24 1886 958 1753 Fragments arranged by size: 1886 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2301 1056 Size: 1245 4327 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2614 2649 2783 4232 1239 Size: 1375 35 134 1449 2579 Fragments arranged by size: 2579 1449 1375 134 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3697 4562 4860 3697 Size: 865 298 4409 Fragments arranged by size: 4409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4235 882 Size: 617 2736 2219 Fragments arranged by size: 2736 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3363 493 Size: 711 2159 2702 Fragments arranged by size: 2702 2159 711 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2072 2322 3379 3761 Size: 53 83 186 936 539 250 1057 382 Cuts at: 3761 4396 275 Size: 635 1451 Fragments arranged by size: 1451 1057 936 635 539 382 250 186 83 53 BsaWI w'CCGG_w Cuts at: 4005 4403 4677 4824 5235 4005 Size: 398 274 147 411 4342 Fragments arranged by size: 4342 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2285 2315 1178 Size: 30 165 30 882 30 4435 Fragments arranged by size: 4435 882 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5572 BseYI C'CCAG_C Cuts at: 2033 2266 2524 2624 2746 4775 5271 2033 Size: 233 258 100 122 2029 496 2334 Fragments arranged by size: 2334 2029 496 258 233 122 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2811 1163 Size: 573 1075 3924 Fragments arranged by size: 3924 1075 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3022 3783 4811 1540 Size: 275 186 1021 761 1028 2301 Fragments arranged by size: 2301 1028 1021 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2619 3633 3718 4789 2619 Size: 1014 85 1071 3402 Fragments arranged by size: 3402 1071 1014 85 BsmI GAATG_Cn' Cuts at: 3083 3176 3083 Size: 93 5479 BsmBI CGTCTCn'nnnn_ Cuts at: 2100 2100 Size: 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2167 2379 2619 2682 2690 2831 2891 Size: 399 377 212 240 63 8 141 60 Cuts at: 2891 3633 3718 4789 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 377 240 212 141 85 63 60 8 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 2589 3461 5191 2589 Size: 872 1730 2970 Fragments arranged by size: 2970 1730 872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2823 2927 844 Size: 1979 104 3489 Fragments arranged by size: 3489 1979 104 BsrBI CCG'CTC Cuts at: 1394 1823 2697 3459 1394 Size: 429 874 762 3507 Fragments arranged by size: 3507 874 762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4067 4241 65 Size: 1183 2819 174 1396 Fragments arranged by size: 2819 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4216 4403 1700 Size: 110 2406 187 2869 Fragments arranged by size: 2869 2406 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5572 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstXI CCAn_nnnn'nTGG Cuts at: 2673 2761 2673 Size: 88 5484 BstYI r'GATC_y Cuts at: 1972 2570 2902 3269 3654 3671 5123 5209 5221 Size: 598 332 367 385 17 1452 86 12 Cuts at: 5221 5567 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 598 385 367 346 332 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5020 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4833 Fragments arranged by size: 4833 683 56 BtsI GCAGTG_nn' Cuts at: 3088 3882 3902 3088 Size: 794 20 4758 Fragments arranged by size: 4758 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DraIII CAC_nnn'GTG Cuts at: 2242 2242 Size: 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2027 4579 809 Size: 507 711 2552 1802 Fragments arranged by size: 2552 1802 711 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2451 2514 3019 Size: 54 1425 50 275 18 621 63 505 Cuts at: 3019 3908 5537 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 621 505 275 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3019 1537 Size: 275 1207 4090 Fragments arranged by size: 4090 1207 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3502 1353 Size: 2149 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2242 4159 4533 4679 387 Size: 1064 138 653 1917 374 146 1280 Fragments arranged by size: 1917 1280 1064 653 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2614 2649 2783 3635 4232 5019 Size: 393 477 505 35 134 852 597 787 Cuts at: 5019 5287 1239 Size: 268 1524 Fragments arranged by size: 1524 852 787 597 505 477 393 268 134 35 EcoO109I rG'GnC_Cy Cuts at: 2029 2029 Size: 5572 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 FspI TGC'GCA Cuts at: 4078 4078 Size: 5572 HaeII r_GCGC'y Cuts at: 1678 2325 2346 2852 4719 1678 Size: 647 21 506 1867 2531 Fragments arranged by size: 2531 1867 647 506 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2562 2594 4213 4245 4287 Size: 32 135 32 990 32 1619 32 42 Cuts at: 4287 4319 1373 Size: 32 2626 Fragments arranged by size: 2626 1619 990 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2916 3162 669 Size: 988 281 978 246 3079 Fragments arranged by size: 3079 988 978 281 246 HindIII A'AGCT_T Cuts at: 748 2274 748 Size: 1526 4046 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KasI G'GCGC_C Cuts at: 2321 2321 Size: 5572 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2753 2760 4686 4870 1305 Size: 1448 7 1926 184 2007 Fragments arranged by size: 2007 1926 1448 184 7 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3750 Fragments arranged by size: 3750 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2715 3530 3889 4048 518 Size: 648 1549 815 359 159 2042 Fragments arranged by size: 2042 1549 815 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2449 2669 2845 2988 3665 4813 5058 1606 Size: 843 220 176 143 677 1148 245 2120 Fragments arranged by size: 2120 1148 843 677 245 220 176 143 NarI GG'CG_CC Cuts at: 2322 2322 Size: 5572 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5020 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NheI G'CTAG_C Cuts at: 1052 2297 1052 Size: 1245 4327 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2930 4475 1132 Size: 97 1701 1545 2229 Fragments arranged by size: 2229 1701 1545 97 PasI CC'CwG_GG Cuts at: 1348 2683 1348 Size: 1335 4237 PciI A'CATG_T Cuts at: 4471 4471 Size: 5572 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5572 PfoI T'CCnGG_A Cuts at: 2858 2858 Size: 5572 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2029 2029 Size: 5572 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PstI C_TGCA'G Cuts at: 830 2924 830 Size: 2094 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 PvuII CAG'CTG Cuts at: 2449 2449 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SanDI GG'GwC_CC Cuts at: 2029 2029 Size: 5572 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 3820 5532 3820 Size: 1712 3860 SfcI C'TryA_G Cuts at: 826 1045 2146 2287 2920 4055 4736 4927 826 Size: 219 1101 141 633 1135 681 191 1471 Fragments arranged by size: 1471 1135 1101 681 633 219 191 141 SfoI GGC'GCC Cuts at: 2323 2323 Size: 5572 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2899 1872 Size: 1027 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3676 4577 4839 820 Size: 197 926 1733 901 262 1553 Fragments arranged by size: 1733 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 3011 513 Size: 552 1946 3074 Fragments arranged by size: 3074 1946 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2387 3599 3616 3769 3954 4399 325 Size: 1255 807 1212 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1212 807 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3818 5530 96 Size: 274 80 33 51 1443 1841 1712 138 Fragments arranged by size: 1841 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5532 5564 5532 Size: 32 5540 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5572 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 2638 2446 Size: 192 5380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2897 1870 Size: 1027 4545 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2073 3380 276 Size: 53 83 186 936 539 1307 2468 Fragments arranged by size: 2468 1307 936 539 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI