(Circular) (Six-base) MAPSORT of: pFN21AB7587.seq Check: 2111 from: 1 to: 5377 pFN21AB7587 5377 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 278 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2720 1937 Size: 783 4594 Acc65I G'GTAC_C Cuts at: 2698 2698 Size: 5377 AclI AA'CG_TT Cuts at: 1563 2676 3504 3877 1563 Size: 1113 828 373 3063 Fragments arranged by size: 3063 1113 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 3440 4824 5092 1632 Size: 704 1104 1384 268 1917 Fragments arranged by size: 1917 1384 1104 704 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5180 AflIII A'CryG_T Cuts at: 1203 4276 5253 1203 Size: 3073 977 1327 Fragments arranged by size: 3073 1327 977 AgeI A'CCGG_T Cuts at: 4208 4208 Size: 5377 AhdI GACnn_n'nnGTC Cuts at: 4106 4106 Size: 5377 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5377 AlwNI CAG_nnn'CTG Cuts at: 2200 2350 2407 3120 4692 2200 Size: 150 57 713 1572 2885 Fragments arranged by size: 2885 1572 713 150 57 ApaI G_GGCC'C Cuts at: 2151 2466 2151 Size: 315 5062 ApaLI G'TGCA_C Cuts at: 2529 3434 4590 2529 Size: 905 1156 3316 Fragments arranged by size: 3316 1156 905 ApoI r'AATT_y Cuts at: 1493 2686 2912 3064 5173 1493 Size: 1193 226 152 2109 1697 Fragments arranged by size: 2109 1697 1193 226 152 AseI AT'TA_AT Cuts at: 160 1032 3931 160 Size: 872 2899 1606 Fragments arranged by size: 2899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2177 2702 1870 Size: 73 192 42 525 4545 Fragments arranged by size: 4545 525 192 73 42 BamHI G'GATC_C Cuts at: 2707 3074 2707 Size: 367 5010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2476 2698 4153 618 Size: 325 217 627 689 222 1455 1842 Fragments arranged by size: 1842 1455 689 627 325 222 217 BanII G_rGCy'C Cuts at: 1391 2124 2135 2151 2466 2696 1391 Size: 733 11 16 315 230 4072 Fragments arranged by size: 4072 733 315 230 16 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3568 3602 1903 Size: 34 1631 34 3678 Fragments arranged by size: 3678 1631 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3269 4479 3269 Size: 1210 4167 BclI T'GATC_A Cuts at: 1548 1548 Size: 5377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2309 3988 136 Size: 107 122 71 631 605 637 1679 1525 Fragments arranged by size: 1679 1525 637 631 605 122 107 71 BglII A'GATC_T Cuts at: 5372 5372 Size: 5377 BlpI GC'TnA_GC Cuts at: 2411 2794 2411 Size: 383 4994 Bme1580I G_kGCm'C Cuts at: 1790 2151 2466 2481 2533 3438 4594 1790 Size: 361 315 15 52 905 1156 2573 Fragments arranged by size: 2573 1156 905 361 315 52 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 4066 442 Size: 416 53 83 399 24 1691 958 1753 Fragments arranged by size: 1753 1691 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2384 2555 4037 1239 Size: 830 315 171 1482 2579 Fragments arranged by size: 2579 1482 830 315 171 Bpu10I CC'TnA_GC Cuts at: 2129 2129 Size: 5377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 2270 3502 4367 4665 2174 Size: 96 1232 865 298 2886 Fragments arranged by size: 2886 1232 865 298 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4040 882 Size: 617 2541 2219 Fragments arranged by size: 2541 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3168 493 Size: 711 1964 2702 Fragments arranged by size: 2702 1964 711 BsaBI GATnn'nnATC Cuts at: 5371 5371 Size: 5377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3184 3566 4201 275 Size: 53 83 186 936 1651 382 635 1451 Fragments arranged by size: 1651 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3810 4208 4482 4629 5040 3810 Size: 398 274 147 411 4147 Fragments arranged by size: 4147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2489 2519 2585 2615 1178 Size: 30 165 30 1086 30 66 30 3940 Fragments arranged by size: 3940 1086 165 66 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 1186 Size: 1258 4119 BseYI C'CCAG_C Cuts at: 2167 2466 2480 4580 5076 2167 Size: 299 14 2100 496 2468 Fragments arranged by size: 2468 2100 496 299 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2582 1163 Size: 573 846 3958 Fragments arranged by size: 3958 846 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3588 4616 1540 Size: 275 186 826 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2184 2533 3438 3523 4594 2124 Size: 60 349 905 85 1071 2907 Fragments arranged by size: 2907 1071 905 349 85 60 BsmI GAATG_Cn' Cuts at: 2492 2888 2981 2492 Size: 396 93 4888 Fragments arranged by size: 4888 396 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2135 2151 2184 2466 2481 2533 Size: 399 334 11 16 33 282 15 52 Cuts at: 2533 2696 3438 3523 4594 1391 Size: 163 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 334 282 163 85 52 33 16 15 11 BspEI T'CCGG_A Cuts at: 5040 5040 Size: 5377 BspHI T'CATG_A Cuts at: 3266 4996 3266 Size: 1730 3647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2604 2732 844 Size: 1235 525 128 3489 Fragments arranged by size: 3489 1235 525 128 BsrBI CCG'CTC Cuts at: 1394 1823 2113 3264 1394 Size: 429 290 1151 3507 Fragments arranged by size: 3507 1151 429 290 BsrDI GCAATG_nn' Cuts at: 65 1248 3872 4046 65 Size: 1183 2624 174 1396 Fragments arranged by size: 2624 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2247 4021 4208 1700 Size: 110 437 1774 187 2869 Fragments arranged by size: 2869 1774 437 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5377 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5377 BssSI C'ACGA_G Cuts at: 3437 4449 3437 Size: 1012 4365 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5377 BstBI TT'CG_AA Cuts at: 2094 4181 2094 Size: 2087 3290 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5377 BstYI r'GATC_y Cuts at: 1972 2364 2707 3074 3459 3476 4928 5014 5026 Size: 392 343 367 385 17 1452 86 12 Cuts at: 5026 5372 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 392 385 367 346 343 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2204 2241 513 Size: 552 939 200 37 3649 Fragments arranged by size: 3649 939 552 200 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4638 Fragments arranged by size: 4638 683 56 BtsI GCAGTG_nn' Cuts at: 2380 2533 2893 3687 3707 2380 Size: 153 360 794 20 4050 Fragments arranged by size: 4050 794 360 153 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5342 DraI TTT'AAA Cuts at: 2681 2681 Size: 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4384 809 Size: 507 3068 1802 Fragments arranged by size: 3068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2824 3713 Size: 54 1425 50 275 18 429 565 889 Cuts at: 3713 5342 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 565 429 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2824 1537 Size: 275 1012 4090 Fragments arranged by size: 4090 1012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 3307 1353 Size: 959 995 3423 Fragments arranged by size: 3423 995 959 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3964 4338 4484 387 Size: 1064 138 2375 374 146 1280 Fragments arranged by size: 2375 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2122 2122 Size: 5377 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2336 2384 2555 3440 4037 4824 Size: 393 437 267 48 171 885 597 787 Cuts at: 4824 5092 1239 Size: 268 1524 Fragments arranged by size: 1524 885 787 597 437 393 268 267 171 48 EcoNI CCTnn'n_nnAGG Cuts at: 2423 2423 Size: 5377 EcoO109I rG'GnC_Cy Cuts at: 2140 2147 2148 2172 2404 2463 2140 Size: 7 1 24 232 59 5054 Fragments arranged by size: 5054 232 59 24 7 1 EcoRI G'AATT_C Cuts at: 2686 3064 2686 Size: 378 4999 FspI TGC'GCA Cuts at: 2372 3883 2372 Size: 1511 3866 HaeII r_GCGC'y Cuts at: 1678 2398 2505 2547 4524 1678 Size: 720 107 42 1977 2531 Fragments arranged by size: 2531 1977 720 107 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4018 4050 4092 4124 1373 Size: 32 135 32 2446 32 42 32 2626 Fragments arranged by size: 2626 2446 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2721 2967 669 Size: 988 281 783 246 3079 Fragments arranged by size: 3079 988 783 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5377 HpaI GTT'AAC Cuts at: 2967 2967 Size: 5377 KpnI G_GTAC'C Cuts at: 2702 2702 Size: 5377 MfeI C'AATT_G Cuts at: 2976 2976 Size: 5377 MluI A'CGCG_T Cuts at: 5253 5253 Size: 5377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4491 4675 1305 Size: 3186 184 2007 Fragments arranged by size: 3186 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3555 Fragments arranged by size: 3555 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2630 3335 3694 3853 518 Size: 648 1464 705 359 159 2042 Fragments arranged by size: 2042 1464 705 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2347 2793 3470 4618 4863 1606 Size: 741 446 677 1148 245 2120 Fragments arranged by size: 2120 1148 741 677 446 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3886 Fragments arranged by size: 3886 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5377 NotI GC'GGCC_GC Cuts at: 2824 2824 Size: 5377 NspI r_CATG'y Cuts at: 1132 1229 2735 4280 1132 Size: 97 1506 1545 2229 Fragments arranged by size: 2229 1545 1506 97 PasI CC'CwG_GG Cuts at: 1348 2144 2552 1348 Size: 796 408 4173 Fragments arranged by size: 4173 796 408 PciI A'CATG_T Cuts at: 4276 4276 Size: 5377 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5377 PmeI GTTT'AAAC Cuts at: 2681 2681 Size: 5377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3478 4959 4991 3446 Size: 32 1481 32 3832 Fragments arranged by size: 3832 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2172 2172 Size: 5377 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5377 PsiI TTA'TAA Cuts at: 2947 2947 Size: 5377 PspOMI G'GGCC_C Cuts at: 2147 2462 2147 Size: 315 5062 PstI C_TGCA'G Cuts at: 830 2513 2729 830 Size: 1683 216 3478 Fragments arranged by size: 3478 1683 216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5377 SacI G_AGCT'C Cuts at: 2124 2124 Size: 5377 SalI G'TCGA_C Cuts at: 1936 2719 1936 Size: 783 4594 SanDI GG'GwC_CC Cuts at: 2172 2172 Size: 5377 SbfI CC_TGCA'GG Cuts at: 2729 2729 Size: 5377 ScaI AGT'ACT Cuts at: 3625 5337 3625 Size: 1712 3665 SfcI C'TryA_G Cuts at: 826 1045 2509 2580 2725 3860 4541 4732 826 Size: 219 1464 71 145 1135 681 191 1471 Fragments arranged by size: 1471 1464 1135 681 219 191 145 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5377 SmaI CCC'GGG Cuts at: 1872 2704 1872 Size: 832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 2285 3481 4382 4644 820 Size: 197 926 210 132 1196 901 262 1553 Fragments arranged by size: 1553 1196 926 901 262 210 197 132 SnaBI TAC'GTA Cuts at: 493 3168 493 Size: 2675 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5377 SphI G_CATG'C Cuts at: 1132 2735 1132 Size: 1603 3774 SspI AAT'ATT Cuts at: 5 52 3301 5 Size: 47 3249 2081 Fragments arranged by size: 3249 2081 47 StuI AGG'CCT Cuts at: 1517 2303 1517 Size: 786 4591 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 2816 513 Size: 552 939 351 461 3074 Fragments arranged by size: 3074 939 552 461 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3404 3421 3574 3759 4204 325 Size: 1255 1824 17 153 185 445 1498 Fragments arranged by size: 1824 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 3623 5335 Size: 274 80 33 51 1443 408 1238 1712 Cuts at: 5335 96 Size: 138 Fragments arranged by size: 1712 1443 1238 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 5337 5369 2489 Size: 32 2816 32 2497 Fragments arranged by size: 2816 2497 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5377 XbaI T'CTAG_A Cuts at: 2713 2713 Size: 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5377 XmaI C'CCGG_G Cuts at: 1870 2702 1870 Size: 832 4545 XmnI GAAnn'nnTTC Cuts at: 3506 3506 Size: 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 276 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI