Restriction map for pFN21AB7583
 (Circular) (Six-base) MAPSORT of: pFN21AB7583.seq  Check: 3513  from: 1  to: 5368



pFN21AB7583  5368 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:39  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3178     278 
   Size:         53      83     186     936    1642    2468

  Fragments arranged by size: 

                2468    1642     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2275    2711    1937 
   Size:        338     436    4594

  Fragments arranged by size: 

                4594     436     338
 
Acc65I G'GTAC_C

Cuts at:   2689    2689 
   Size:       5368
 
AclI AA'CG_TT

Cuts at:   1563    3495    3868    1563 
   Size:       1932     373    3063

  Fragments arranged by size: 

                3063    1932     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2354    2411    3431    4815    5083    1632 
   Size:        722      57    1020    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1020     722     268      57
 
AfeI AGC'GCT

Cuts at:   2465    2465 
   Size:       5368
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5171
 
AflIII A'CryG_T

Cuts at:   1203    4267    5244    1203 
   Size:       3064     977    1327

  Fragments arranged by size: 

                3064    1327     977
 
AgeI A'CCGG_T

Cuts at:   4199    4199 
   Size:       5368
 
AhdI GACnn_n'nnGTC

Cuts at:   4097    4097 
   Size:       5368
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5368
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5368
 
AlwNI CAG_nnn'CTG

Cuts at:   2038    3111    4683    2038 
   Size:       1073    1572    2723

  Fragments arranged by size: 

                2723    1572    1073
 
ApaLI G'TGCA_C

Cuts at:   3425    4581    3425 
   Size:       1156    4212
 
ApoI r'AATT_y

Cuts at:   1493    2677    2903    3055    5164    1493 
   Size:       1184     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1184     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3922     160 
   Size:        872    2890    1606

  Fragments arranged by size: 

                2890    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5368
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2693    1870 
   Size:         73     750    4545

  Fragments arranged by size: 

                4545     750      73
 
BamHI G'GATC_C

Cuts at:   2424    2698    3065    2424 
   Size:        274     367    4727

  Fragments arranged by size: 

                4727     367     274
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2251    2689    4144     618 
   Size:        325     217     627     464     438    1455    1842

  Fragments arranged by size: 

                1842    1455     627     464     438     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2215    2687    1391 
   Size:        824     472    4072

  Fragments arranged by size: 

                4072     824     472
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4397
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3559    3593    1903 
   Size:         34    1622      34    3678

  Fragments arranged by size: 

                3678    1622      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3260    4470    3260 
   Size:       1210    4158
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5368
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    3979     136 
   Size:        107     122      71     631     605     337    1970    1525

  Fragments arranged by size: 

                1970    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5363    5363 
   Size:       5368
 
BlpI GC'TnA_GC

Cuts at:   2785    2785 
   Size:       5368
 
Bme1580I G_kGCm'C

Cuts at:   1790    3429    4585    1790 
   Size:       1639    1156    2573

  Fragments arranged by size: 

                2573    1639    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2500    1318 
   Size:       1182    4186
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2267    3099    4057 
   Size:        416      53      83     399      24     850     832     958

Cuts at:   4057     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     850     832     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5368
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2063    2081    2252    2423    2557    4028    1239 
   Size:        824      18     171     171     134    1471    2579

  Fragments arranged by size: 

                2579    1471     824     171     171     134      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3493    4358    4656    3493 
   Size:        865     298    4205

  Fragments arranged by size: 

                4205     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2669    4031     882 
   Size:        617    1170    1362    2219

  Fragments arranged by size: 

                2219    1362    1170     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3159     493 
   Size:        711    1955    2702

  Fragments arranged by size: 

                2702    1955     711
 
BsaBI GATnn'nnATC

Cuts at:   2027    5362    2027 
   Size:       3335    2033
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3175    3557    4192     275 
   Size:         53      83     186     936    1642     382     635    1451

  Fragments arranged by size: 

                1642    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2427    3801    4199    4473    4620    5031    2427 
   Size:       1374     398     274     147     411    2764

  Fragments arranged by size: 

                2764    1374     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5143

  Fragments arranged by size: 

                5143     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2446    1186 
   Size:       1260    4108
 
BseYI C'CCAG_C

Cuts at:   2410    4571    5067    2410 
   Size:       2161     496    2711

  Fragments arranged by size: 

                2711    2161     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4795
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2052    2818    3579    4607    1540 
   Size:        275     186      51     766     761    1028    2301

  Fragments arranged by size: 

                2301    1028     766     761     275     186      51
 
BsiHKAI G_wGCw'C

Cuts at:   2510    3429    3514    4585    2510 
   Size:        919      85    1071    3293

  Fragments arranged by size: 

                3293    1071     919      85
 
BsmI GAATG_Cn'

Cuts at:   2879    2972    2879 
   Size:         93    5275
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2458    2458 
   Size:       5368
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2215    2510    2687    3429    3514    4585    1391 
   Size:        399     425     295     177     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     425     399     295     177      85
            
 
BspEI T'CCGG_A

Cuts at:   2427    5031    2427 
   Size:       2604    2764
 
BspHI T'CATG_A

Cuts at:   3257    4987    3257 
   Size:       1730    3638
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2723     844 
   Size:       1879    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2065    2569    3255    1394 
   Size:        429     242     504     686    3507

  Fragments arranged by size: 

                3507     686     504     429     242
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3863    4037      65 
   Size:       1183    2615     174    1396

  Fragments arranged by size: 

                2615    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4012    4199    1700 
   Size:        110    2202     187    2869

  Fragments arranged by size: 

                2869    2202     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5368
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5368
 
BssSI C'ACGA_G

Cuts at:   3428    4440    3428 
   Size:       1012    4356
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5368
 
BstBI TT'CG_AA

Cuts at:   4172    4172 
   Size:       5368
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5368
 
BstYI r'GATC_y

Cuts at:   1972    2424    2698    3065    3450    3467    4919    5005    5017 
   Size:        452     274     367     385      17    1452      86      12

Cuts at:   5017    5363    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     452     385     367     346     274      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3877

  Fragments arranged by size: 

                3877     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4629

  Fragments arranged by size: 

                4629     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2554    2599    2884    3678    3698    2554 
   Size:         45     285     794      20    4224

  Fragments arranged by size: 

                4224     794     285      45      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5368
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5333
 
DraI TTT'AAA

Cuts at:   2672    2672 
   Size:       5368
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4375     809 
   Size:        507    3059    1802

  Fragments arranged by size: 

                3059    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2049    2815    3704 
   Size:         54    1425      50     275      18     219     766     889

Cuts at:   3704    5333       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     766     275     219      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2049    2815    1537 
   Size:        275     237     766    4090

  Fragments arranged by size: 

                4090     766     275     237
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2387    3298    1353 
   Size:       1034     911    3423

  Fragments arranged by size: 

                3423    1034     911
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3955    4329    4475     387 
   Size:       1064     138    2366     374     146    1280

  Fragments arranged by size: 

                2366    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2063    2081    2252    2354    2411    2423    2557 
   Size:        393     431      18     171     102      57      12     134

Cuts at:   2557    3431    4028    4815    5083    1239 
   Size:        874     597     787     268    1524

  Fragments arranged by size: 

                1524     874     787     597     431     393     268     171
                 134     102      57      18      12
 
EcoO109I rG'GnC_Cy

Cuts at:   2261    2261 
   Size:       5368
 
EcoRI G'AATT_C

Cuts at:   2677    3055    2677 
   Size:        378    4990
 
FspI TGC'GCA

Cuts at:   3874    3874 
   Size:       5368
 
HaeII r_GCGC'y

Cuts at:   1678    2467    4515    1678 
   Size:        789    2048    2531

  Fragments arranged by size: 

                2531    2048     789
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4009    4041    4083    4115    1373 
   Size:         32     135      32    2437      32      42      32    2626

  Fragments arranged by size: 

                2626    2437     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2276    2497    2712    2958     669 
   Size:        988     281     338     221     215     246    3079

  Fragments arranged by size: 

                3079     988     338     281     246     221     215
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5368
 
HpaI GTT'AAC

Cuts at:   2958    2958 
   Size:       5368
 
KpnI G_GTAC'C

Cuts at:   2693    2693 
   Size:       5368
 
MfeI C'AATT_G

Cuts at:   2967    2967 
   Size:       5368
 
MluI A'CGCG_T

Cuts at:   5244    5244 
   Size:       5368
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2493    2499    4482    4666    1305 
   Size:       1188       6    1983     184    2007

  Fragments arranged by size: 

                2007    1983    1188     184       6
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3546

  Fragments arranged by size: 

                3546    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2033    2091    3326    3685    3844     518 
   Size:        648     867      58    1235     359     159    2042

  Fragments arranged by size: 

                2042    1235     867     648     359     159      58
 
MspA1I CmG'CkG

Cuts at:   1606    2401    2784    3461    4609    4854    1606 
   Size:        795     383     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     795     677     383     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3877

  Fragments arranged by size: 

                3877     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5368
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5368
 
NotI GC'GGCC_GC

Cuts at:   2815    2815 
   Size:       5368
 
NspI r_CATG'y

Cuts at:   1132    1229    2581    2726    4271    1132 
   Size:         97    1352     145    1545    2229

  Fragments arranged by size: 

                2229    1545    1352     145      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5368
 
PciI A'CATG_T

Cuts at:   4267    4267 
   Size:       5368
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5368
 
PmeI GTTT'AAAC

Cuts at:   2672    2672 
   Size:       5368
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3437    3469    4950    4982    3437 
   Size:         32    1481      32    3823

  Fragments arranged by size: 

                3823    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2261    2261 
   Size:       5368
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5368
 
PsiI TTA'TAA

Cuts at:   2938    2938 
   Size:       5368
 
PstI C_TGCA'G

Cuts at:    830    2640    2720     830 
   Size:       1810      80    3478

  Fragments arranged by size: 

                3478    1810      80
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5368
 
PvuII CAG'CTG

Cuts at:   2401    2401 
   Size:       5368
 
SalI G'TCGA_C

Cuts at:   1936    2274    2710    1936 
   Size:        338     436    4594

  Fragments arranged by size: 

                4594     436     338
 
SanDI GG'GwC_CC

Cuts at:   2261    2261 
   Size:       5368
 
SbfI CC_TGCA'GG

Cuts at:   2720    2720 
   Size:       5368
 
ScaI AGT'ACT

Cuts at:   3616    5328    3616 
   Size:       1712    3656
 
SfcI C'TryA_G

Cuts at:    826    1045    2636    2716    3851    4532    4723     826 
   Size:        219    1591      80    1135     681     191    1471

  Fragments arranged by size: 

                1591    1471    1135     681     219     191      80
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5368
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5368
 
SmaI CCC'GGG

Cuts at:   1872    2695    1872 
   Size:        823    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3472    4373    4635     820 
   Size:        197     926    1529     901     262    1553

  Fragments arranged by size: 

                1553    1529     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3159     493 
   Size:       2666    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5368
 
SphI G_CATG'C

Cuts at:   1132    2581    2726    1132 
   Size:       1449     145    3774

  Fragments arranged by size: 

                3774    1449     145
 
SspI AAT'ATT

Cuts at:      5      52    3292       5 
   Size:         47    3240    2081

  Fragments arranged by size: 

                3240    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5368
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2807     513 
   Size:        552     939     803    3074

  Fragments arranged by size: 

                3074     939     803     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3395    3412    3565    3750    4195     325 
   Size:       1255    1815      17     153     185     445    1498

  Fragments arranged by size: 

                1815    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3614    5326      96 
   Size:        274      80      33      51    1443    1637    1712     138

  Fragments arranged by size: 

                1712    1637    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5328    5360    5328 
   Size:         32    5336
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5368
 
XbaI T'CTAG_A

Cuts at:   2704    2704 
   Size:       5368
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5368
 
XmaI C'CCGG_G

Cuts at:   1870    2693    1870 
   Size:        823    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3497    3497 
   Size:       5368
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3176     276 
   Size:         53      83     186     936    1642    2468

  Fragments arranged by size: 

                2468    1642     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI    SanDI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com