(Circular) (Six-base) MAPSORT of: pFN21AB7574.seq Check: 6455 from: 1 to: 5548 pFN21AB7574 5548 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 2847 3675 4048 1563 Size: 1284 828 373 3063 Fragments arranged by size: 3063 1284 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2769 3611 4995 5263 1632 Size: 1137 842 1384 268 1917 Fragments arranged by size: 1917 1384 1137 842 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5548 AlwNI CAG_nnn'CTG Cuts at: 2506 3291 4863 2506 Size: 785 1572 3191 Fragments arranged by size: 3191 1572 785 ApaLI G'TGCA_C Cuts at: 2669 3605 4761 2669 Size: 936 1156 3456 Fragments arranged by size: 3456 1156 936 ApoI r'AATT_y Cuts at: 1493 2225 2857 3083 3235 5344 1493 Size: 732 632 226 152 2109 1697 Fragments arranged by size: 2109 1697 732 632 226 152 AseI AT'TA_AT Cuts at: 160 1032 2130 4102 160 Size: 872 1098 1972 1606 Fragments arranged by size: 1972 1606 1098 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2252 2873 1870 Size: 73 309 621 4545 Fragments arranged by size: 4545 621 309 73 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2079 2869 4324 618 Size: 325 217 627 292 790 1455 1842 Fragments arranged by size: 1842 1455 790 627 325 292 217 BanII G_rGCy'C Cuts at: 1391 2867 1391 Size: 1476 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2661 928 Size: 971 762 3815 Fragments arranged by size: 3815 971 762 BbvCI CC'TCA_GC Cuts at: 2814 2814 Size: 5548 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4159 136 Size: 107 122 71 631 605 2487 1525 Fragments arranged by size: 2487 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2243 5543 2243 Size: 3300 2248 BlpI GC'TnA_GC Cuts at: 2499 2965 2499 Size: 466 5082 Bme1580I G_kGCm'C Cuts at: 1790 2673 3609 4765 1790 Size: 883 936 1156 2573 Fragments arranged by size: 2573 1156 936 883 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2031 2300 3279 Size: 416 53 83 399 24 614 269 979 Cuts at: 3279 4237 442 Size: 958 1753 Fragments arranged by size: 1753 979 958 614 416 399 269 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2187 2187 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2601 4208 1239 Size: 1362 1607 2579 Fragments arranged by size: 2579 1607 1362 Bpu10I CC'TnA_GC Cuts at: 2814 2814 Size: 5548 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3673 4538 4836 3673 Size: 865 298 4385 Fragments arranged by size: 4385 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2079 2179 4211 882 Size: 617 580 100 2032 2219 Fragments arranged by size: 2219 2032 617 580 100 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3355 3737 4372 275 Size: 53 83 186 936 1822 382 635 1451 Fragments arranged by size: 1822 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3981 4379 4653 4800 5211 3981 Size: 398 274 147 411 4318 Fragments arranged by size: 4318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2025 2055 1178 Size: 30 165 30 622 30 4671 Fragments arranged by size: 4671 622 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2075 1186 Size: 847 42 4659 Fragments arranged by size: 4659 847 42 BseYI C'CCAG_C Cuts at: 4751 5247 4751 Size: 496 5052 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4975 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2021 2998 3759 4787 1540 Size: 275 186 20 977 761 1028 2301 Fragments arranged by size: 2301 1028 977 761 275 186 20 BsiHKAI G_wGCw'C Cuts at: 2500 2673 2684 2780 3609 3694 4765 2500 Size: 173 11 96 829 85 1071 3283 Fragments arranged by size: 3283 1071 829 173 96 85 11 BsmI GAATG_Cn' Cuts at: 2574 3059 3152 2574 Size: 485 93 4970 Fragments arranged by size: 4970 485 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2057 2057 Size: 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2500 2673 2684 2780 2867 3609 3694 Size: 399 710 173 11 96 87 742 85 Cuts at: 3694 4765 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 710 399 173 96 87 85 11 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 844 Size: 2059 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2466 2601 4043 4217 65 Size: 1183 1218 135 1442 174 1396 Fragments arranged by size: 1442 1396 1218 1183 174 135 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 4192 4379 1700 Size: 110 210 2172 187 2869 Fragments arranged by size: 2869 2172 210 187 110 BsrGI T'GTAC_A Cuts at: 96 2146 96 Size: 2050 3498 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstXI CCAn_nnnn'nTGG Cuts at: 2098 2098 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2233 2243 2387 2878 3245 3630 3647 5099 Size: 261 10 144 491 367 385 17 1452 Cuts at: 5099 5185 5197 5543 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 491 385 367 346 261 144 86 17 12 10 Bsu36I CC'TnA_GG Cuts at: 2796 2796 Size: 5548 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4996 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 3064 3858 3878 3064 Size: 794 20 4734 Fragments arranged by size: 4734 794 20 ClaI AT'CG_AT Cuts at: 1554 2697 1554 Size: 1143 4405 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2018 2995 3884 Size: 54 1425 50 275 18 188 977 889 Cuts at: 3884 5513 8 Size: 1629 43 Fragments arranged by size: 1629 1425 977 889 275 188 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2018 2995 1537 Size: 275 206 977 4090 Fragments arranged by size: 4090 977 275 206 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 2770 3478 1353 Size: 824 593 708 3423 Fragments arranged by size: 3423 824 708 593 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4509 4655 387 Size: 1064 138 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2601 2769 3611 4208 4995 5263 1239 Size: 393 969 168 842 597 787 268 1524 Fragments arranged by size: 1524 969 842 787 597 393 268 168 EcoO109I rG'GnC_Cy Cuts at: 2104 2760 2792 2104 Size: 656 32 4860 Fragments arranged by size: 4860 656 32 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2849 2849 Size: 5548 FspI TGC'GCA Cuts at: 4054 4054 Size: 5548 HaeII r_GCGC'y Cuts at: 1678 4695 1678 Size: 3017 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2076 2108 2767 2799 4189 Size: 32 135 32 504 32 659 32 1390 Cuts at: 4189 4221 4263 4295 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1390 659 504 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2517 2892 3138 669 Size: 988 281 579 375 246 3079 Fragments arranged by size: 3079 988 579 375 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 2517 3138 2517 Size: 621 4927 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4662 4846 1305 Size: 3357 184 2007 Fragments arranged by size: 3357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3726 Fragments arranged by size: 3726 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 3506 3865 4024 518 Size: 648 930 1410 359 159 2042 Fragments arranged by size: 2042 1410 930 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2506 2964 3641 4789 5034 1606 Size: 411 489 458 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 489 458 411 245 NaeI GCC'GGC Cuts at: 2022 2022 Size: 5548 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4996 NdeI CA'TA_TG Cuts at: 387 2548 387 Size: 2161 3387 NgoMIV G'CCGG_C Cuts at: 2020 2020 Size: 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2906 4451 1132 Size: 97 1677 1545 2229 Fragments arranged by size: 2229 1677 1545 97 PacI TTA_AT'TAA Cuts at: 2487 2487 Size: 5548 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5548 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PfoI T'CCnGG_A Cuts at: 2046 2046 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2760 2760 Size: 5548 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PstI C_TGCA'G Cuts at: 830 2900 830 Size: 2070 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2506 2506 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SfcI C'TryA_G Cuts at: 826 1045 2432 2896 4031 4712 4903 826 Size: 219 1387 464 1135 681 191 1471 Fragments arranged by size: 1471 1387 1135 681 464 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2254 2875 1872 Size: 382 621 4545 Fragments arranged by size: 4545 621 382 SmlI C'TyrA_G Cuts at: 820 1017 1943 3652 4553 4815 820 Size: 197 926 1709 901 262 1553 Fragments arranged by size: 1709 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 2582 1517 Size: 1065 4483 StyI C'CwwG_G Cuts at: 513 1065 2987 513 Size: 552 1922 3074 Fragments arranged by size: 3074 1922 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3575 3592 3745 3930 4375 325 Size: 1255 1995 17 153 185 445 1498 Fragments arranged by size: 1995 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2146 3794 5506 Size: 274 80 33 51 1443 169 1648 1712 Cuts at: 5506 96 Size: 138 Fragments arranged by size: 1712 1648 1443 274 169 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2044 2044 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2252 2873 1870 Size: 382 621 4545 Fragments arranged by size: 4545 621 382 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI