(Circular) (Six-base) MAPSORT of: pFN21AB7572.seq Check: 5050 from: 1 to: 5545 pFN21AB7572 5545 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2116 2116 Size: 5545 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2343 2888 1937 Size: 406 545 4594 Fragments arranged by size: 4594 545 406 Acc65I G'GTAC_C Cuts at: 2866 2866 Size: 5545 AclI AA'CG_TT Cuts at: 1563 2499 3672 4045 1563 Size: 936 1173 373 3063 Fragments arranged by size: 3063 1173 936 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3608 4992 5260 1632 Size: 1976 1384 268 1917 Fragments arranged by size: 1976 1917 1384 268 AfeI AGC'GCT Cuts at: 2483 2483 Size: 5545 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 4444 5421 1203 Size: 3241 977 1327 Fragments arranged by size: 3241 1327 977 AgeI A'CCGG_T Cuts at: 4376 4376 Size: 5545 AhdI GACnn_n'nnGTC Cuts at: 4274 4274 Size: 5545 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5545 AlwNI CAG_nnn'CTG Cuts at: 2087 3288 4860 2087 Size: 1201 1572 2772 Fragments arranged by size: 2772 1572 1201 ApaI G_GGCC'C Cuts at: 2039 2121 2039 Size: 82 5463 ApaLI G'TGCA_C Cuts at: 2684 3602 4758 2684 Size: 918 1156 3471 Fragments arranged by size: 3471 1156 918 ApoI r'AATT_y Cuts at: 1493 2854 3080 3232 5341 1493 Size: 1361 226 152 2109 1697 Fragments arranged by size: 2109 1697 1361 226 152 AseI AT'TA_AT Cuts at: 160 1032 4099 160 Size: 872 3067 1606 Fragments arranged by size: 3067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2663 2870 1870 Size: 73 720 207 4545 Fragments arranged by size: 4545 720 207 73 BamHI G'GATC_C Cuts at: 2717 2875 3242 2717 Size: 158 367 5020 Fragments arranged by size: 5020 367 158 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2433 2507 2705 2866 4321 Size: 325 217 627 646 74 198 161 1455 Cuts at: 4321 618 Size: 1842 Fragments arranged by size: 1842 1455 646 627 325 217 198 161 74 BanII G_rGCy'C Cuts at: 1391 2039 2121 2834 2864 1391 Size: 648 82 713 30 4072 Fragments arranged by size: 4072 713 648 82 30 BbeI G_GCGC'C Cuts at: 2511 2709 2511 Size: 198 5347 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4574 BbvCI CC'TCA_GC Cuts at: 2427 2522 2427 Size: 95 5450 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2730 2764 3736 3770 1903 Size: 34 793 34 972 34 3678 Fragments arranged by size: 3678 972 793 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4647 3437 Size: 1210 4335 BclI T'GATC_A Cuts at: 1548 2190 1548 Size: 642 4903 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2167 2738 4156 Size: 107 122 71 631 605 495 571 1418 Cuts at: 4156 136 Size: 1525 Fragments arranged by size: 1525 1418 631 605 571 495 122 107 71 BglII A'GATC_T Cuts at: 5540 5540 Size: 5545 BlpI GC'TnA_GC Cuts at: 2561 2962 2561 Size: 401 5144 Bme1580I G_kGCm'C Cuts at: 1790 2012 2039 2074 2121 2249 2436 2688 3606 Size: 222 27 35 47 128 187 252 918 Cuts at: 3606 4762 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 918 252 222 187 128 47 35 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3276 4234 442 Size: 416 53 83 399 24 1859 958 1753 Fragments arranged by size: 1859 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4205 1239 Size: 2966 2579 Bpu10I CC'TnA_GC Cuts at: 2100 2427 2522 2100 Size: 327 95 5123 Fragments arranged by size: 5123 327 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3670 4535 4833 3670 Size: 865 298 4382 Fragments arranged by size: 4382 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2392 4208 882 Size: 617 893 1816 2219 Fragments arranged by size: 2219 1816 893 617 BsaAI yAC'GTr Cuts at: 493 1204 3336 493 Size: 711 2132 2702 Fragments arranged by size: 2702 2132 711 BsaBI GATnn'nnATC Cuts at: 5539 5539 Size: 5545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2508 2706 3352 3734 Size: 53 83 186 936 975 198 646 382 Cuts at: 3734 4369 275 Size: 635 1451 Fragments arranged by size: 1451 975 936 646 635 382 198 186 83 53 BsaWI w'CCGG_w Cuts at: 2553 2720 3978 4376 4650 4797 5208 2553 Size: 167 1258 398 274 147 411 2890 Fragments arranged by size: 2890 1258 411 398 274 167 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5320 Fragments arranged by size: 5320 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 2147 2163 1186 Size: 960 1 16 4568 Fragments arranged by size: 4568 960 16 1 BseYI C'CCAG_C Cuts at: 2075 2080 2242 4748 5244 2075 Size: 5 162 2506 496 2376 Fragments arranged by size: 2506 2376 496 162 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2294 1163 Size: 573 558 4414 Fragments arranged by size: 4414 573 558 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2554 2681 2769 2995 3756 4784 Size: 275 186 553 127 88 226 761 1028 Cuts at: 4784 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 553 275 226 186 127 88 BsiHKAI G_wGCw'C Cuts at: 2580 2688 3606 3691 4762 2580 Size: 108 918 85 1071 3363 Fragments arranged by size: 3363 1071 918 108 85 BsmI GAATG_Cn' Cuts at: 3056 3149 3056 Size: 93 5452 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2039 2074 2121 2249 2436 2580 Size: 399 222 27 35 47 128 187 144 Cuts at: 2580 2688 2834 2864 3606 3691 4762 1391 Size: 108 146 30 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 222 187 146 144 128 108 85 47 35 30 27 BspEI T'CCGG_A Cuts at: 2720 5208 2720 Size: 2488 3057 BspHI T'CATG_A Cuts at: 3434 5164 3434 Size: 1730 3815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2116 2900 844 Size: 1272 784 3489 Fragments arranged by size: 3489 1272 784 BsrBI CCG'CTC Cuts at: 1394 1823 2795 3432 1394 Size: 429 972 637 3507 Fragments arranged by size: 3507 972 637 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4040 4214 65 Size: 1183 2792 174 1396 Fragments arranged by size: 2792 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2385 2680 4189 4376 1700 Size: 110 575 295 1509 187 2869 Fragments arranged by size: 2869 1509 575 295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 2744 1925 Size: 819 4726 BssSI C'ACGA_G Cuts at: 3605 4617 3605 Size: 1012 4533 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5545 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5545 BstXI CCAn_nnnn'nTGG Cuts at: 2242 2812 2242 Size: 570 4975 BstYI r'GATC_y Cuts at: 1972 2717 2875 3242 3627 3644 5096 5182 5194 Size: 745 158 367 385 17 1452 86 12 Cuts at: 5194 5540 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 745 385 367 346 158 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2016 2016 Size: 5545 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4993 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4806 Fragments arranged by size: 4806 683 56 BtsI GCAGTG_nn' Cuts at: 3061 3855 3875 3061 Size: 794 20 4731 Fragments arranged by size: 4731 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2849 2849 Size: 5545 DraIII CAC_nnn'GTG Cuts at: 2180 2180 Size: 5545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4552 809 Size: 507 3236 1802 Fragments arranged by size: 3236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2643 2678 2766 Size: 54 1425 50 275 18 813 35 88 Cuts at: 2766 2992 3881 5510 8 Size: 226 889 1629 43 Fragments arranged by size: 1629 1425 889 813 275 226 88 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 2678 2766 2992 1537 Size: 275 866 88 226 4090 Fragments arranged by size: 4090 866 275 226 88 EarI CTCTTCn'nnn_ Cuts at: 1353 3475 1353 Size: 2122 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2570 4132 4506 4652 387 Size: 1064 138 981 1562 374 146 1280 Fragments arranged by size: 1562 1280 1064 981 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3608 4205 4992 5260 1239 Size: 393 1976 597 787 268 1524 Fragments arranged by size: 1976 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2426 2426 Size: 5545 EcoO109I rG'GnC_Cy Cuts at: 2036 2118 2211 2360 2651 2692 2036 Size: 82 93 149 291 41 4889 Fragments arranged by size: 4889 291 149 93 82 41 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 FspI TGC'GCA Cuts at: 4051 4051 Size: 5545 HaeII r_GCGC'y Cuts at: 1678 2087 2485 2511 2709 4692 1678 Size: 409 398 26 198 1983 2531 Fragments arranged by size: 2531 1983 409 398 198 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4186 4218 4260 4292 1373 Size: 32 135 32 2614 32 42 32 2626 Fragments arranged by size: 2626 2614 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2503 2889 3135 669 Size: 988 281 565 386 246 3079 Fragments arranged by size: 3079 988 565 386 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5545 HpaI GTT'AAC Cuts at: 2503 3135 2503 Size: 632 4913 KasI G'GCGC_C Cuts at: 2507 2705 2507 Size: 198 5347 KpnI G_GTAC'C Cuts at: 2870 2870 Size: 5545 MfeI C'AATT_G Cuts at: 3144 3144 Size: 5545 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4659 4843 1305 Size: 3354 184 2007 Fragments arranged by size: 3354 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3723 Fragments arranged by size: 3723 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2810 3503 3862 4021 518 Size: 648 1644 693 359 159 2042 Fragments arranged by size: 2042 1644 693 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 2961 3638 4786 5031 1606 Size: 657 698 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 677 657 245 NarI GG'CG_CC Cuts at: 2508 2706 2508 Size: 198 5347 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4993 NdeI CA'TA_TG Cuts at: 387 387 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2903 4448 1132 Size: 97 1674 1545 2229 Fragments arranged by size: 2229 1674 1545 97 PasI CC'CwG_GG Cuts at: 1348 2216 2281 1348 Size: 868 65 4612 Fragments arranged by size: 4612 868 65 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 2449 1108 Size: 1341 4204 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2360 2692 2360 Size: 332 5213 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5545 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PspOMI G'GGCC_C Cuts at: 2035 2117 2035 Size: 82 5463 PstI C_TGCA'G Cuts at: 830 2897 830 Size: 2067 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SanDI GG'GwC_CC Cuts at: 2692 2692 Size: 5545 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 3793 5505 3793 Size: 1712 3833 SexAI A'CCwGG_T Cuts at: 2176 2176 Size: 5545 SfcI C'TryA_G Cuts at: 826 1045 2893 4028 4709 4900 826 Size: 219 1848 1135 681 191 1471 Fragments arranged by size: 1848 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2509 2707 2509 Size: 198 5347 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2665 2872 1872 Size: 793 207 4545 Fragments arranged by size: 4545 793 207 SmlI C'TyrA_G Cuts at: 820 1017 1943 3649 4550 4812 820 Size: 197 926 1706 901 262 1553 Fragments arranged by size: 1706 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 3469 5 Size: 47 3417 2081 Fragments arranged by size: 3417 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5545 StyI C'CwwG_G Cuts at: 513 1065 2984 513 Size: 552 1919 3074 Fragments arranged by size: 3074 1919 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3572 3589 3742 3927 4372 325 Size: 1255 1992 17 153 185 445 1498 Fragments arranged by size: 1992 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 3791 5503 Size: 274 80 33 51 1443 618 1196 1712 Cuts at: 5503 96 Size: 138 Fragments arranged by size: 1712 1443 1196 618 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5505 5537 5505 Size: 32 5513 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5545 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2239 2239 Size: 5545 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5545 XmaI C'CCGG_G Cuts at: 1870 2663 2870 1870 Size: 793 207 4545 Fragments arranged by size: 4545 793 207 XmnI GAAnn'nnTTC Cuts at: 3674 3674 Size: 5545 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SrfI SwaI