Restriction map for
pFN21AB7571
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SfcI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tru9I Tsp509I Van91I
XbaI XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 319 GGCGCC G[GA]^CG[CT]C
AfaI 21 GTAC GT^AC
AlwI 47 GGATC GGATC(4/5)
ApaI 570 GGGCCC GGGCC^C
AspHI 127 GAGCTC G[AT]GC[AT]^C
AspI 168 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 252 GGACC G^G[AT]CC
BbeI 319 GGCGCC GGCGC^C
BbvCI 575 CCTCAGC CCTCAGC(-5/-2)
BfaI 136 CTAG C^TAG
BpmI 226 CTGGAG CTGGAG(16/14)
BsaHI 319 GGCGCC G[GA]^CG[CT]C
BseRI 457 GAGGAG GAGGAG(10/8)
BsiEI 351 CGGCCG CG[GA][CT]^CG
BsiHKAI 127 GAGCTC G[AT]GC[AT]^C
BsmAI 460 GAGAC GTCTC(1/5)
BsrFI 39 GCCGGC [GA]^CCGG[CT]
BsrI 171 CCAGT ACTGG(1/-1)
BstEII 165 GGTGACC G^GT[ACGT]ACC
BstPI 165 GGTGACC G^GT[ACGT]ACC
BstXI 300 CCACATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
EaeI 351 CGGCCG [CT]^GGCC[GA]
EagI 351 CGGCCG C^GGCCG
EclXI 351 CGGCCG C^GGCCG
Eco52I 351 CGGCCG C^GGCCG
EcoO65I 165 GGTGACC G^GT[ACGT]ACC
Hin1I 319 GGCGCC G[GA]^CG[CT]C
HphI 165 GGTGA GGTGA(8/7)
KasI 319 GGCGCC G^GCGCC
MaeI 136 CTAG C^TAG
MaeIII 166 GTGAC ^GT[ACGT]AC
MslI 301 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 345 CAGCGG C[AC]G^C[GT]G
NaeI 39 GCCGGC GCC^GGC
NarI 319 GGCGCC GG^CGCC
NgoMIV 39 GCCGGC G^CCGGC
NspI 275 ACATGC [GA]CATG^[CT]
PaeR7I 124 CTCGAG C^TCGAG
PflFI 168 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 251 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 21 GTAC GT^AC
SacI 127 GAGCTC GAGCT^C
SapI 129 GCTCTTC GCTCTTC(1/4)
SexAI 254 ACCTGGT A^CC[AT]GGT
SfoI 319 GGCGCC GGC^GCC
SgrAI 38 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 124 CTCGAG C^T[CT][GA]AG
StuI 155 AGGCCT AGG^CCT
TfiI 427 GAATC G^A[AT]TC
Tsp45I 166 GTGAC ^GT[GC]AC
TspRI 214 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 168 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 124 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 440 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
578 CAGCACCTG
BanI 99 GGTGCC G^G[CT][GA]CC
319 GGCGCC
BbsI 186 GAAGAC GAAGAC(2/6)
292 GAAGAC
BbvI 231 GCAGC GCAGC(8/12)
344 GCAGC
BpuAI 186 GAAGAC GAAGAC(2/6)
292 GAAGAC
DdeI 431 CTGAG C^T[ACGT]AG
576 CTCAG
DpnII 48 GATC ^GATC
57 GATC
DraII 251 AGGACCT [GA]G^G[ACGT]CC[CT]
570 GGGCCCC
EcoO109I 251 AGGACCT [GA]G^G[ACGT]CC[CT]
570 GGGCCCC
FauI 36 CCCGC CCCGC(4/6)
323 CCCGC
MboI 48 GATC ^GATC
57 GATC
NdeII 48 GATC ^GATC
57 GATC
Sau3AI 48 GATC ^GATC
57 GATC
SfaNI 218 GCATC GCATC(5/9)
262 GCATC
SmaI 116 CCCGGG CCC^GGG
567 CCCGGG
TaqI 125 TCGA T^CGA
221 TCGA
TseI 231 GCAGC G^C[AT]GC
344 GCAGC
TthHB8I 125 TCGA T^CGA
221 TCGA
XmaCI 116 CCCGGG C^CCGGG
567 CCCGGG
XmaI 116 CCCGGG C^CCGGG
567 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BcnI BmyI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EarI EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MboII MnlI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI