Restriction map for
pFN21AB7564
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AlwNI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BamHI BanI
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 183 CGCG CG^CG
AcyI 256 GACGCC G[GA]^CG[CT]C
AfaI 687 GTAC GT^AC
Alw44I 444 GTGCAC G^TGCAC
Aor51HI 557 AGCGCT AGC^GCT
ApaLI 444 GTGCAC G^TGCAC
AvrII 105 CCTAGG C^CTAGG
BbsI 10 GTCTTC GAAGAC(2/6)
BfaI 106 CTAG C^TAG
BlnI 105 CCTAGG C^CTAGG
BpuAI 10 GTCTTC GAAGAC(2/6)
BsaHI 256 GACGCC G[GA]^CG[CT]C
BsaI 39 GGTCTC GGTCTC(1/5)
BsiEI 299 CGGTCG CG[GA][CT]^CG
BsmAI 40 GTCTC GTCTC(1/5)
BsmI 512 GCATTC GAATGC(1/-1)
BspMI 689 ACCTGC ACCTGC(4/8)
BstUI 183 CGCG CG^CG
CpoI 44 CGGTCCG CG^G[AT]CCG
DdeI 802 CTGAG C^T[ACGT]AG
DpnII 308 GATC ^GATC
DsaI 591 CCATGG C^C[GA][CT]GG
Eco47III 557 AGCGCT AGC^GCT
HgaI 256 GACGC GACGC(5/10)
Hin1I 256 GACGCC G[GA]^CG[CT]C
MaeI 106 CTAG C^TAG
MboI 308 GATC ^GATC
MvnI 183 CGCG CG^CG
NcoI 591 CCATGG C^CATGG
NdeII 308 GATC ^GATC
NspI 395 GCATGC [GA]CATG^[CT]
PpuMI 680 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 687 GTAC GT^AC
RsrII 44 CGGTCCG CG^G[AT]CCG
SacI 61 GAGCTC GAGCT^C
Sau3AI 308 GATC ^GATC
SexAI 425 ACCAGGT A^CC[AT]GGT
SmlI 412 CTCAAG C^T[CT][GA]AG
SphI 395 GCATGC GCATG^C
XcmI 5 CCAAAGTCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
ApaI 264 GGGCCC GGGCC^C
666 GGGCCC
AspHI 61 GAGCTC G[AT]GC[AT]^C
444 GTGCAC
AvaII 45 GGTCC G^G[AT]CC
681 GGACC
BbrPI 333 CACGTG CAC^GTG
694 CACGTG
BpmI 194 CTGGAG CTGGAG(16/14)
568 CTGGAG
BsaAI 333 CACGTG [CT]AC^GT[GA]
694 CACGTG
BsiHKAI 61 GAGCTC G[AT]GC[AT]^C
444 GTGCAC
BsrI 747 ACTGG ACTGG(1/-1)
97 CCAGT
EarI 21 GAAGAG CTCTTC(1/4)
486 GAAGAG
EcoNI 756 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
801 CCTGAGTGAGG
EcoT14I 105 CCTAGG C^C[AT][AT]GG
591 CCATGG
HaeII 293 AGCGCC [GA]GCGC^[CT]
557 AGCGCT
HinfI 343 GACTC G^A[ACGT]TC
571 GAGTC
Ksp632I 21 GAAGAG CTCTTC(1/4)
486 GAAGAG
MaeIII 362 GTGAC ^GT[ACGT]AC
407 GTCAC
MslI 93 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
355 CACAGCTGTG
NlaIII 396 CATG CATG^
592 CATG
PleI 343 GACTC GAGTC(4/5)
571 GAGTC
PmaCI 333 CACGTG CAC^GTG
694 CACGTG
PmlI 333 CACGTG CAC^GTG
694 CACGTG
PstI 388 CTGCAG CTGCA^G
760 CTGCAG
PvuII 51 CAGCTG CAG^CTG
357 CAGCTG
SfcI 388 CTGCAG C^T[GA][CT]AG
760 CTGCAG
StyI 105 CCTAGG C^C[AT][AT]GG
591 CCATGG
TaqI 310 TCGA T^CGA
479 TCGA
Tsp45I 362 GTGAC ^GT[GC]AC
407 GTCAC
TspRI 98 CAGTG CA[GC]TG[ACGT][ACGT]^
702 CAGTG
TthHB8I 310 TCGA T^CGA
479 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaI BanII BbvI
BcnI BmyI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MboII MnlI MspA1I MspI
MvaI MwoI NciI NlaIV Sau96I
ScrFI TseI