(Circular) (Six-base) MAPSORT of: pFN21AB7564.seq Check: 5841 from: 1 to: 5530 pFN21AB7564 5530 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3340 278 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2873 1937 Size: 936 4594 Acc65I G'GTAC_C Cuts at: 2851 2851 Size: 5530 AclI AA'CG_TT Cuts at: 1563 3657 4030 1563 Size: 2094 373 3063 Fragments arranged by size: 3063 2094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 2606 3593 4977 5245 1632 Size: 878 96 987 1384 268 1917 Fragments arranged by size: 1917 1384 987 878 268 96 AfeI AGC'GCT Cuts at: 2564 2564 Size: 5530 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5333 AflIII A'CryG_T Cuts at: 1203 4429 5406 1203 Size: 3226 977 1327 Fragments arranged by size: 3226 1327 977 AgeI A'CCGG_T Cuts at: 4361 4361 Size: 5530 AhdI GACnn_n'nnGTC Cuts at: 4259 4259 Size: 5530 AleI CACnn'nnGTG Cuts at: 1166 2364 1166 Size: 1198 4332 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2631 1184 Size: 1447 4083 AlwNI CAG_nnn'CTG Cuts at: 3273 4845 3273 Size: 1572 3958 ApaI G_GGCC'C Cuts at: 2273 2675 2273 Size: 402 5128 ApaLI G'TGCA_C Cuts at: 2449 3587 4743 2449 Size: 1138 1156 3236 Fragments arranged by size: 3236 1156 1138 ApoI r'AATT_y Cuts at: 1493 2839 3065 3217 5326 1493 Size: 1346 226 152 2109 1697 Fragments arranged by size: 2109 1697 1346 226 152 AseI AT'TA_AT Cuts at: 160 1032 4084 160 Size: 872 3052 1606 Fragments arranged by size: 3052 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2234 2249 2855 1870 Size: 73 192 99 15 606 4545 Fragments arranged by size: 4545 606 192 99 73 15 AvrII C'CTAG_G Cuts at: 2110 2110 Size: 5530 BamHI G'GATC_C Cuts at: 2860 3227 2860 Size: 367 5163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 4306 618 Size: 325 217 627 1064 1455 1842 Fragments arranged by size: 1842 1455 1064 627 325 217 BanII G_rGCy'C Cuts at: 1391 2070 2256 2273 2675 2849 1391 Size: 679 186 17 402 174 4072 Fragments arranged by size: 4072 679 402 186 174 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2008 928 Size: 971 109 4450 Fragments arranged by size: 4450 971 109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3721 3755 1903 Size: 34 50 34 1700 34 3678 Fragments arranged by size: 3678 1700 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3422 4632 3422 Size: 1210 4320 BclI T'GATC_A Cuts at: 1548 1548 Size: 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4141 136 Size: 107 122 71 631 605 2469 1525 Fragments arranged by size: 2469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5525 5525 Size: 5530 BlpI GC'TnA_GC Cuts at: 2947 2947 Size: 5530 Bme1580I G_kGCm'C Cuts at: 1790 2273 2453 2675 2713 3591 4747 1790 Size: 483 180 222 38 878 1156 2573 Fragments arranged by size: 2573 1156 878 483 222 180 38 BmgBI CAC'GTC Cuts at: 1318 2500 1318 Size: 1182 4348 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2096 3261 4219 Size: 416 53 83 399 24 679 1165 958 Cuts at: 4219 442 Size: 1753 Fragments arranged by size: 1753 1165 958 679 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5530 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2220 2594 4190 1239 Size: 981 374 1596 2579 Fragments arranged by size: 2579 1596 981 374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2402 3655 4520 4818 2402 Size: 1253 865 298 3114 Fragments arranged by size: 3114 1253 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2050 4193 882 Size: 617 551 2143 2219 Fragments arranged by size: 2219 2143 617 551 BsaAI yAC'GTr Cuts at: 493 1204 2340 2701 3321 493 Size: 711 1136 361 620 2702 Fragments arranged by size: 2702 1136 711 620 361 BsaBI GATnn'nnATC Cuts at: 5524 5524 Size: 5530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 3337 3719 4354 Size: 53 83 186 936 729 1075 382 635 Cuts at: 4354 275 Size: 1451 Fragments arranged by size: 1451 1075 936 729 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3963 4361 4635 4782 5193 3963 Size: 398 274 147 411 4300 Fragments arranged by size: 4300 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2675 2705 1178 Size: 30 165 30 1272 30 4003 Fragments arranged by size: 4003 1272 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2047 2217 2828 2831 2834 1186 Size: 858 3 170 611 3 3 3882 Fragments arranged by size: 3882 858 611 170 3 3 3 BseYI C'CCAG_C Cuts at: 2058 2559 4733 5229 2058 Size: 501 2174 496 2359 Fragments arranged by size: 2359 2174 501 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2362 2624 2681 1163 Size: 573 626 262 57 4012 Fragments arranged by size: 4012 626 573 262 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2307 2980 3741 4769 1540 Size: 275 186 306 673 761 1028 2301 Fragments arranged by size: 2301 1028 761 673 306 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 2453 3591 3676 4747 2070 Size: 383 1138 85 1071 2853 Fragments arranged by size: 2853 1138 1071 383 85 BsmI GAATG_Cn' Cuts at: 2517 3041 3134 2517 Size: 524 93 4913 Fragments arranged by size: 4913 524 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2256 2273 2453 2675 2713 2849 Size: 399 280 186 17 180 222 38 136 Cuts at: 2849 3591 3676 4747 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 280 222 186 180 136 85 38 17 BspEI T'CCGG_A Cuts at: 5193 5193 Size: 5530 BspHI T'CATG_A Cuts at: 3419 5149 3419 Size: 1730 3800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2703 2885 844 Size: 1859 182 3489 Fragments arranged by size: 3489 1859 182 BsrBI CCG'CTC Cuts at: 1394 1823 3417 1394 Size: 429 1594 3507 Fragments arranged by size: 3507 1594 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4025 4199 65 Size: 1183 2777 174 1396 Fragments arranged by size: 2777 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4174 4361 1700 Size: 110 2364 187 2869 Fragments arranged by size: 2869 2364 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5530 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5530 BssSI C'ACGA_G Cuts at: 3590 4602 3590 Size: 1012 4518 BstAPI GCAn_nnn'nTGC Cuts at: 794 2401 2457 2541 794 Size: 1607 56 84 3783 Fragments arranged by size: 3783 1607 84 56 BstBI TT'CG_AA Cuts at: 4334 4334 Size: 5530 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5530 BstYI r'GATC_y Cuts at: 1972 2860 3227 3612 3629 5081 5167 5179 5525 Size: 888 367 385 17 1452 86 12 346 Cuts at: 5525 1972 Size: 1977 Fragments arranged by size: 1977 1452 888 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2596 513 Size: 552 1531 3447 Fragments arranged by size: 3447 1531 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4791 Fragments arranged by size: 4791 683 56 BtsI GCAGTG_nn' Cuts at: 3046 3840 3860 3046 Size: 794 20 4716 Fragments arranged by size: 4716 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5495 DraI TTT'AAA Cuts at: 2834 2834 Size: 5530 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4537 809 Size: 507 3221 1802 Fragments arranged by size: 3221 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2977 3866 5495 Size: 54 1425 50 275 18 1147 889 1629 Cuts at: 5495 8 Size: 43 Fragments arranged by size: 1629 1425 1147 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2977 1537 Size: 275 1165 4090 Fragments arranged by size: 4090 1165 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2486 3460 1353 Size: 668 465 974 3423 Fragments arranged by size: 3423 974 668 465 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4117 4491 4637 387 Size: 1064 138 2528 374 146 1280 Fragments arranged by size: 2528 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2068 2068 Size: 5530 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2220 2510 2594 2606 3593 4190 4977 Size: 393 588 290 84 12 987 597 787 Cuts at: 4977 5245 1239 Size: 268 1524 Fragments arranged by size: 1524 987 787 597 588 393 290 268 84 12 EcoNI CCTnn'n_nnAGG Cuts at: 2765 2810 2765 Size: 45 5485 EcoO109I rG'GnC_Cy Cuts at: 2269 2270 2672 2686 2269 Size: 1 402 14 5113 Fragments arranged by size: 5113 402 14 1 EcoRI G'AATT_C Cuts at: 2839 3217 2839 Size: 378 5152 FspI TGC'GCA Cuts at: 4036 4036 Size: 5530 HaeII r_GCGC'y Cuts at: 1678 2302 2566 4677 1678 Size: 624 264 2111 2531 Fragments arranged by size: 2531 2111 624 264 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2304 2336 4171 4203 4245 Size: 32 135 32 732 32 1835 32 42 Cuts at: 4245 4277 1373 Size: 32 2626 Fragments arranged by size: 2626 1835 732 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2874 3120 669 Size: 988 281 936 246 3079 Fragments arranged by size: 3079 988 936 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5530 HpaI GTT'AAC Cuts at: 3120 3120 Size: 5530 KpnI G_GTAC'C Cuts at: 2855 2855 Size: 5530 MfeI C'AATT_G Cuts at: 3129 3129 Size: 5530 MluI A'CGCG_T Cuts at: 5406 5406 Size: 5530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4644 4828 1305 Size: 3339 184 2007 Fragments arranged by size: 3339 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3708 Fragments arranged by size: 3708 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 2364 3488 3847 4006 518 Size: 648 936 262 1124 359 159 2042 Fragments arranged by size: 2042 1124 936 648 359 262 159 MspA1I CmG'CkG Cuts at: 1606 2058 2169 2175 2364 2629 2946 3623 4771 Size: 452 111 6 189 265 317 677 1148 Cuts at: 4771 5016 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 452 317 265 245 189 111 6 NcoI C'CATG_G Cuts at: 513 1065 2596 513 Size: 552 1531 3447 Fragments arranged by size: 3447 1531 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5530 NotI GC'GGCC_GC Cuts at: 2977 2977 Size: 5530 NspI r_CATG'y Cuts at: 1132 1229 2404 2888 4433 1132 Size: 97 1175 484 1545 2229 Fragments arranged by size: 2229 1545 1175 484 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5530 PciI A'CATG_T Cuts at: 4429 4429 Size: 5530 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5530 PfoI T'CCnGG_A Cuts at: 2070 2070 Size: 5530 PmeI GTTT'AAAC Cuts at: 2834 2834 Size: 5530 PmlI CAC'GTG Cuts at: 2340 2701 2340 Size: 361 5169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 5112 5144 3599 Size: 32 1481 32 3985 Fragments arranged by size: 3985 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2686 2686 Size: 5530 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5530 PsiI TTA'TAA Cuts at: 3100 3100 Size: 5530 PspOMI G'GGCC_C Cuts at: 2269 2671 2269 Size: 402 5128 PstI C_TGCA'G Cuts at: 830 2397 2769 2882 830 Size: 1567 372 113 3478 Fragments arranged by size: 3478 1567 372 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5530 PvuII CAG'CTG Cuts at: 2058 2364 2058 Size: 306 5224 RsrII CG'GwC_CG Cuts at: 2050 2050 Size: 5530 SacI G_AGCT'C Cuts at: 2070 2070 Size: 5530 SalI G'TCGA_C Cuts at: 1936 2872 1936 Size: 936 4594 SbfI CC_TGCA'GG Cuts at: 2882 2882 Size: 5530 ScaI AGT'ACT Cuts at: 3778 5490 3778 Size: 1712 3818 SexAI A'CCwGG_T Cuts at: 2430 2430 Size: 5530 SfcI C'TryA_G Cuts at: 826 1045 2393 2765 2878 4013 4694 4885 826 Size: 219 1348 372 113 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 681 372 219 191 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5530 SmaI CCC'GGG Cuts at: 1872 2857 1872 Size: 985 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2417 3634 4535 4797 820 Size: 197 926 474 1217 901 262 1553 Fragments arranged by size: 1553 1217 926 901 474 262 197 SnaBI TAC'GTA Cuts at: 493 3321 493 Size: 2828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5530 SphI G_CATG'C Cuts at: 1132 2404 2888 1132 Size: 1272 484 3774 Fragments arranged by size: 3774 1272 484 SspI AAT'ATT Cuts at: 5 52 3454 5 Size: 47 3402 2081 Fragments arranged by size: 3402 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5530 StyI C'CwwG_G Cuts at: 513 1065 2110 2596 2969 513 Size: 552 1045 486 373 3074 Fragments arranged by size: 3074 1045 552 486 373 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2573 2693 3557 3574 3727 3912 Size: 1255 458 535 120 864 17 153 185 Cuts at: 3912 4357 325 Size: 445 1498 Fragments arranged by size: 1498 1255 864 535 458 445 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3776 5488 96 Size: 274 80 33 51 1443 1799 1712 138 Fragments arranged by size: 1799 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5490 5522 5490 Size: 32 5498 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5530 XbaI T'CTAG_A Cuts at: 2866 2866 Size: 5530 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2017 Size: 5530 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5530 XmaI C'CCGG_G Cuts at: 1870 2855 1870 Size: 985 4545 XmnI GAAnn'nnTTC Cuts at: 3659 3659 Size: 5530 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3338 276 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI SacII SanDI SapI SfiI SfoI SrfI SwaI