(Circular) (Six-base) MAPSORT of: pFN21AB7562.seq Check: 9738 from: 1 to: 5527 pFN21AB7562 5527 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3337 278 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2870 1937 Size: 933 4594 Acc65I G'GTAC_C Cuts at: 2848 2848 Size: 5527 AclI AA'CG_TT Cuts at: 1563 3654 4027 1563 Size: 2091 373 3063 Fragments arranged by size: 3063 2091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 3590 4974 5242 1632 Size: 458 1500 1384 268 1917 Fragments arranged by size: 1917 1500 1384 458 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5330 AflIII A'CryG_T Cuts at: 1203 4426 5403 1203 Size: 3223 977 1327 Fragments arranged by size: 3223 1327 977 AgeI A'CCGG_T Cuts at: 4358 4358 Size: 5527 AhdI GACnn_n'nnGTC Cuts at: 4256 4256 Size: 5527 AleI CACnn'nnGTG Cuts at: 1166 2013 1166 Size: 847 4680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2045 2745 1184 Size: 861 700 3966 Fragments arranged by size: 3966 861 700 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2490 2522 2490 Size: 32 5495 AlwNI CAG_nnn'CTG Cuts at: 2200 3270 4842 2200 Size: 1070 1572 2885 Fragments arranged by size: 2885 1572 1070 ApaI G_GGCC'C Cuts at: 2330 2330 Size: 5527 ApaLI G'TGCA_C Cuts at: 3584 4740 3584 Size: 1156 4371 ApoI r'AATT_y Cuts at: 1493 2836 3062 3214 5323 1493 Size: 1343 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 226 152 AseI AT'TA_AT Cuts at: 160 1032 4081 160 Size: 872 3049 1606 Fragments arranged by size: 3049 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 AvaI C'yCGr_G Cuts at: 1870 1943 2383 2630 2852 1870 Size: 73 440 247 222 4545 Fragments arranged by size: 4545 440 247 222 73 BamHI G'GATC_C Cuts at: 2767 2857 3224 2767 Size: 90 367 5070 Fragments arranged by size: 5070 367 90 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2848 4303 618 Size: 325 217 627 1061 1455 1842 Fragments arranged by size: 1842 1455 1061 627 325 217 BanII G_rGCy'C Cuts at: 1391 2292 2322 2330 2846 1391 Size: 901 30 8 516 4072 Fragments arranged by size: 4072 901 516 30 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2559 928 Size: 971 660 3896 Fragments arranged by size: 3896 971 660 BbvCI CC'TCA_GC Cuts at: 2313 2313 Size: 5527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3718 3752 1903 Size: 34 50 34 1697 34 3678 Fragments arranged by size: 3678 1697 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3419 4629 3419 Size: 1210 4317 BclI T'GATC_A Cuts at: 1548 1548 Size: 5527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2213 4138 136 Size: 107 122 71 631 605 541 1925 1525 Fragments arranged by size: 1925 1525 631 605 541 122 107 71 BglII A'GATC_T Cuts at: 5522 5522 Size: 5527 BlpI GC'TnA_GC Cuts at: 2278 2403 2944 2278 Size: 125 541 4861 Fragments arranged by size: 4861 541 125 Bme1580I G_kGCm'C Cuts at: 1790 2258 2330 2459 2587 3588 4744 1790 Size: 468 72 129 128 1001 1156 2573 Fragments arranged by size: 2573 1156 1001 468 129 128 72 BmgBI CAC'GTC Cuts at: 1318 2705 1318 Size: 1387 4140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3258 4216 442 Size: 416 53 83 399 24 1841 958 1753 Fragments arranged by size: 1841 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2268 2387 2400 2435 2573 2657 4187 1239 Size: 1029 119 13 35 138 84 1530 2579 Fragments arranged by size: 2579 1530 1029 138 119 84 35 13 Bpu10I CC'TnA_GC Cuts at: 2313 2313 Size: 5527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2799 3652 4517 4815 2799 Size: 853 865 298 3511 Fragments arranged by size: 3511 865 853 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4190 882 Size: 617 2691 2219 Fragments arranged by size: 2691 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2239 3318 493 Size: 711 1035 1079 2702 Fragments arranged by size: 2702 1079 1035 711 BsaBI GATnn'nnATC Cuts at: 5521 5521 Size: 5527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 3334 3716 4351 Size: 53 83 186 936 600 1201 382 635 Cuts at: 4351 275 Size: 1451 Fragments arranged by size: 1451 1201 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3960 4358 4632 4779 5190 3960 Size: 398 274 147 411 4297 Fragments arranged by size: 4297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 2602 2632 1178 Size: 30 165 30 777 30 392 30 4073 Fragments arranged by size: 4073 777 392 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 2797 1186 Size: 1252 359 3916 Fragments arranged by size: 3916 1252 359 BseYI C'CCAG_C Cuts at: 2097 2301 2398 2441 2519 2594 2659 2771 4730 Size: 204 97 43 78 75 65 112 1959 Cuts at: 4730 5226 2097 Size: 496 2398 Fragments arranged by size: 2398 1959 496 204 112 97 78 75 65 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2219 1163 Size: 573 387 96 4471 Fragments arranged by size: 4471 573 387 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2977 3738 4766 1540 Size: 275 186 976 761 1028 2301 Fragments arranged by size: 2301 1028 976 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 3588 3673 4744 2724 Size: 864 85 1071 3507 Fragments arranged by size: 3507 1071 864 85 BsmI GAATG_Cn' Cuts at: 3038 3131 3038 Size: 93 5434 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2258 2292 2322 2330 2459 2587 2724 Size: 399 468 34 30 8 129 128 137 Cuts at: 2724 2846 3588 3673 4744 1391 Size: 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 468 399 137 129 128 122 85 34 30 8 BspEI T'CCGG_A Cuts at: 5190 5190 Size: 5527 BspHI T'CATG_A Cuts at: 2431 3416 5146 2431 Size: 985 1730 2812 Fragments arranged by size: 2812 1730 985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2737 2882 844 Size: 1893 145 3489 Fragments arranged by size: 3489 1893 145 BsrBI CCG'CTC Cuts at: 1394 1823 2410 3414 1394 Size: 429 587 1004 3507 Fragments arranged by size: 3507 1004 587 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2029 4022 4196 65 Size: 1183 781 1993 174 1396 Fragments arranged by size: 1993 1396 1183 781 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2106 4171 4358 1700 Size: 110 296 2065 187 2869 Fragments arranged by size: 2869 2065 296 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5527 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5527 BssSI C'ACGA_G Cuts at: 2150 3587 4599 2150 Size: 1437 1012 3078 Fragments arranged by size: 3078 1437 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5527 BstBI TT'CG_AA Cuts at: 4331 4331 Size: 5527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5527 BstYI r'GATC_y Cuts at: 1972 2190 2767 2857 3224 3609 3626 5078 5164 Size: 218 577 90 367 385 17 1452 86 Cuts at: 5164 5176 5522 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 577 385 367 346 218 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2259 513 Size: 552 1194 3781 Fragments arranged by size: 3781 1194 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4788 Fragments arranged by size: 4788 683 56 BtsI GCAGTG_nn' Cuts at: 3043 3837 3857 3043 Size: 794 20 4713 Fragments arranged by size: 4713 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5492 DraI TTT'AAA Cuts at: 2831 2831 Size: 5527 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4534 809 Size: 507 3218 1802 Fragments arranged by size: 3218 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2974 3863 5492 Size: 54 1425 50 275 18 1144 889 1629 Cuts at: 5492 8 Size: 43 Fragments arranged by size: 1629 1425 1144 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2974 1537 Size: 275 1162 4090 Fragments arranged by size: 4090 1162 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3457 1353 Size: 2104 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4114 4488 4634 387 Size: 1064 138 2525 374 146 1280 Fragments arranged by size: 2525 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2268 2387 2400 2435 2573 2657 Size: 393 458 178 119 13 35 138 84 Cuts at: 2657 3590 4187 4974 5242 1239 Size: 933 597 787 268 1524 Fragments arranged by size: 1524 933 787 597 458 393 268 178 138 119 84 35 13 EcoNI CCTnn'n_nnAGG Cuts at: 2183 2183 Size: 5527 EcoO109I rG'GnC_Cy Cuts at: 2327 2664 2327 Size: 337 5190 EcoRI G'AATT_C Cuts at: 2836 3214 2836 Size: 378 5149 FspI TGC'GCA Cuts at: 4033 4033 Size: 5527 HaeII r_GCGC'y Cuts at: 1678 2251 4674 1678 Size: 573 2423 2531 Fragments arranged by size: 2531 2423 573 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2180 2212 2255 2287 4168 Size: 32 135 32 608 32 43 32 1881 Cuts at: 4168 4200 4242 4274 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1881 608 135 43 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2871 3117 669 Size: 988 281 933 246 3079 Fragments arranged by size: 3079 988 933 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5527 HpaI GTT'AAC Cuts at: 3117 3117 Size: 5527 KpnI G_GTAC'C Cuts at: 2852 2852 Size: 5527 MfeI C'AATT_G Cuts at: 3126 3126 Size: 5527 MluI A'CGCG_T Cuts at: 5403 5403 Size: 5527 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4641 4825 1305 Size: 3336 184 2007 Fragments arranged by size: 3336 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3705 Fragments arranged by size: 3705 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2013 2241 3485 3844 4003 518 Size: 648 847 228 1244 359 159 2042 Fragments arranged by size: 2042 1244 847 648 359 228 159 MspA1I CmG'CkG Cuts at: 1606 2068 2365 2486 2512 2943 3620 4768 5013 Size: 462 297 121 26 431 677 1148 245 Cuts at: 5013 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 462 431 297 245 121 26 NaeI GCC'GGC Cuts at: 2108 2108 Size: 5527 NcoI C'CATG_G Cuts at: 513 1065 2259 513 Size: 552 1194 3781 Fragments arranged by size: 3781 1194 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5527 NgoMIV G'CCGG_C Cuts at: 2106 2106 Size: 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5527 NotI GC'GGCC_GC Cuts at: 2974 2974 Size: 5527 NspI r_CATG'y Cuts at: 1132 1229 2885 4430 1132 Size: 97 1656 1545 2229 Fragments arranged by size: 2229 1656 1545 97 PasI CC'CwG_GG Cuts at: 1348 2692 1348 Size: 1344 4183 PciI A'CATG_T Cuts at: 4426 4426 Size: 5527 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5527 PmeI GTTT'AAAC Cuts at: 2831 2831 Size: 5527 PmlI CAC'GTG Cuts at: 2239 2239 Size: 5527 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3596 3628 5109 5141 3596 Size: 32 1481 32 3982 Fragments arranged by size: 3982 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2664 2664 Size: 5527 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5527 PsiI TTA'TAA Cuts at: 3097 3097 Size: 5527 PspOMI G'GGCC_C Cuts at: 2326 2326 Size: 5527 PspXI vC'TCGA_Gb Cuts at: 2630 2630 Size: 5527 PstI C_TGCA'G Cuts at: 830 2205 2562 2712 2879 830 Size: 1375 357 150 167 3478 Fragments arranged by size: 3478 1375 357 167 150 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5527 PvuII CAG'CTG Cuts at: 2068 2365 2512 2068 Size: 297 147 5083 Fragments arranged by size: 5083 297 147 SalI G'TCGA_C Cuts at: 1936 2869 1936 Size: 933 4594 SbfI CC_TGCA'GG Cuts at: 2205 2562 2879 2205 Size: 357 317 4853 Fragments arranged by size: 4853 357 317 ScaI AGT'ACT Cuts at: 3775 5487 3775 Size: 1712 3815 SfcI C'TryA_G Cuts at: 826 1045 2201 2558 2708 2875 4010 4691 4882 Size: 219 1156 357 150 167 1135 681 191 Cuts at: 4882 826 Size: 1471 Fragments arranged by size: 1471 1156 1135 681 357 219 191 167 150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5527 SmaI CCC'GGG Cuts at: 1872 2385 2854 1872 Size: 513 469 4545 Fragments arranged by size: 4545 513 469 SmlI C'TyrA_G Cuts at: 820 1017 1943 2630 2814 3631 4532 4794 820 Size: 197 926 687 184 817 901 262 1553 Fragments arranged by size: 1553 926 901 817 687 262 197 184 SnaBI TAC'GTA Cuts at: 493 3318 493 Size: 2825 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5527 SphI G_CATG'C Cuts at: 1132 2885 1132 Size: 1753 3774 SspI AAT'ATT Cuts at: 5 52 3451 5 Size: 47 3399 2081 Fragments arranged by size: 3399 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5527 StyI C'CwwG_G Cuts at: 513 1065 2049 2259 2476 2966 513 Size: 552 984 210 217 490 3074 Fragments arranged by size: 3074 984 552 490 217 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3554 3571 3724 3909 4354 325 Size: 1255 1974 17 153 185 445 1498 Fragments arranged by size: 1974 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3773 5485 96 Size: 274 80 33 51 1443 1796 1712 138 Fragments arranged by size: 1796 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 5487 5519 2534 Size: 32 2921 32 2542 Fragments arranged by size: 2921 2542 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5527 XbaI T'CTAG_A Cuts at: 2863 2863 Size: 5527 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2656 2656 Size: 5527 XhoI C'TCGA_G Cuts at: 1943 2630 1943 Size: 687 4840 XmaI C'CCGG_G Cuts at: 1870 2383 2852 1870 Size: 513 469 4545 Fragments arranged by size: 4545 513 469 XmnI GAAnn'nnTTC Cuts at: 3656 3656 Size: 5527 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3335 276 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI