Restriction map for
pFN21AB7562
(Linear) (Six-base) MAPSORT of: KIBB7562.seq Check: 6995 from: 1 to: 822
KIBB7562.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 822
Size: 85 737
AleI CACnn'nnGTG
Cuts at: 0 8 822
Size: 8 814
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 740 822
Size: 40 700 82
Fragments arranged by size:
700 82 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 485 517 822
Size: 485 32 305
Fragments arranged by size:
485 305 32
AlwNI CAG_nnn'CTG
Cuts at: 0 195 822
Size: 195 627
ApaI G_GGCC'C
Cuts at: 0 325 822
Size: 325 497
AvaI C'yCGr_G
Cuts at: 0 378 625 822
Size: 378 247 197
Fragments arranged by size:
378 247 197
BamHI G'GATC_C
Cuts at: 0 762 822
Size: 762 60
BanII G_rGCy'C
Cuts at: 0 287 317 325 822
Size: 287 30 8 497
Fragments arranged by size:
497 287 30 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 554 822
Size: 554 268
BbvCI CC'TCA_GC
Cuts at: 0 308 822
Size: 308 514
BglI GCCn_nnn'nGGC
Cuts at: 0 208 822
Size: 208 614
BlpI GC'TnA_GC
Cuts at: 0 273 398 822
Size: 273 125 424
Fragments arranged by size:
424 273 125
Bme1580I G_kGCm'C
Cuts at: 0 253 325 454 582 822
Size: 253 72 129 128 240
Fragments arranged by size:
253 240 129 128 72
BmgBI CAC'GTC
Cuts at: 0 700 822
Size: 700 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 382 395 430 568 652 822
Size: 263 119 13 35 138 84 170
Fragments arranged by size:
263 170 138 119 84 35 13
Bpu10I CC'TnA_GC
Cuts at: 0 308 822
Size: 308 514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 822
Size: 794 28
BsaAI yAC'GTr
Cuts at: 0 234 822
Size: 234 588
BsaHI Gr'CG_yC
Cuts at: 0 128 822
Size: 128 694
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 597 627 822
Size: 175 30 392 30 195
Fragments arranged by size:
392 195 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 792 822
Size: 433 359 30
Fragments arranged by size:
433 359 30
BseYI C'CCAG_C
Cuts at: 0 92 296 393 436 514 589 654 766
Size: 92 204 97 43 78 75 65 112
Cuts at: 766 822
Size: 56
Fragments arranged by size:
204 112 97 92 78 75 65 56
43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 214 822
Size: 118 96 608
Fragments arranged by size:
608 118 96
BsiHKAI G_wGCw'C
Cuts at: 0 719 822
Size: 719 103
Bsp1286I G_dGCh'C
Cuts at: 0 253 287 317 325 454 582 719 822
Size: 253 34 30 8 129 128 137 103
Fragments arranged by size:
253 137 129 128 103 34 30 8
BspHI T'CATG_A
Cuts at: 0 426 822
Size: 426 396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 732 822
Size: 732 90
BsrBI CCG'CTC
Cuts at: 0 405 822
Size: 405 417
BsrDI GCAATG_nn'
Cuts at: 0 24 822
Size: 24 798
BsrFI r'CCGG_y
Cuts at: 0 101 822
Size: 101 721
BssSI C'ACGA_G
Cuts at: 0 145 822
Size: 145 677
BstYI r'GATC_y
Cuts at: 0 185 762 822
Size: 185 577 60
Fragments arranged by size:
577 185 60
BtgI C'CryG_G
Cuts at: 0 254 822
Size: 254 568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 263 382 395 430 568 652 822
Size: 85 178 119 13 35 138 84 170
Fragments arranged by size:
178 170 138 119 85 84 35 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 178 822
Size: 178 644
EcoO109I rG'GnC_Cy
Cuts at: 0 322 659 822
Size: 322 337 163
Fragments arranged by size:
337 322 163
HaeII r_GCGC'y
Cuts at: 0 246 822
Size: 246 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 250 282 822
Size: 175 32 43 32 540
Fragments arranged by size:
540 175 43 32 32
MslI CAynn'nnrTG
Cuts at: 0 8 236 822
Size: 8 228 586
Fragments arranged by size:
586 228 8
MspA1I CmG'CkG
Cuts at: 0 63 360 481 507 822
Size: 63 297 121 26 315
Fragments arranged by size:
315 297 121 63 26
NaeI GCC'GGC
Cuts at: 0 103 822
Size: 103 719
NcoI C'CATG_G
Cuts at: 0 254 822
Size: 254 568
NgoMIV G'CCGG_C
Cuts at: 0 101 822
Size: 101 721
PasI CC'CwG_GG
Cuts at: 0 687 822
Size: 687 135
PmlI CAC'GTG
Cuts at: 0 234 822
Size: 234 588
PpuMI rG'GwC_Cy
Cuts at: 0 659 822
Size: 659 163
PspOMI G'GGCC_C
Cuts at: 0 321 822
Size: 321 501
PspXI vC'TCGA_Gb
Cuts at: 0 625 822
Size: 625 197
PstI C_TGCA'G
Cuts at: 0 200 557 707 822
Size: 200 357 150 115
Fragments arranged by size:
357 200 150 115
PvuII CAG'CTG
Cuts at: 0 63 360 507 822
Size: 63 297 147 315
Fragments arranged by size:
315 297 147 63
SbfI CC_TGCA'GG
Cuts at: 0 200 557 822
Size: 200 357 265
Fragments arranged by size:
357 265 200
SfcI C'TryA_G
Cuts at: 0 196 553 703 822
Size: 196 357 150 119
Fragments arranged by size:
357 196 150 119
SmaI CCC'GGG
Cuts at: 0 380 822
Size: 380 442
SmlI C'TyrA_G
Cuts at: 0 625 809 822
Size: 625 184 13
Fragments arranged by size:
625 184 13
StyI C'CwwG_G
Cuts at: 0 44 254 471 822
Size: 44 210 217 351
Fragments arranged by size:
351 217 210 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 529 561 822
Size: 529 32 261
Fragments arranged by size:
529 261 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 651 822
Size: 651 171
XhoI C'TCGA_G
Cuts at: 0 625 822
Size: 625 197
XmaI C'CCGG_G
Cuts at: 0 378 822
Size: 378 444
Enzymes that do cut:
AcuI AleI AlfI AloI AlwNI ApaI AvaI BamHI
BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstYI BtgI Eco57MI EcoNI EcoO109I HaeII Hin4I MslI
MspA1I NaeI NcoI NgoMIV PasI PmlI PpuMI PspOMI
PspXI PstI PvuII SbfI SfcI SmaI SmlI StyI
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI BsaI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XmnI ZraI