(Circular) (Six-base) MAPSORT of: pFN21AB7557.seq Check: 5673 from: 1 to: 5311 pFN21AB7557 5311 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3121 278 Size: 53 83 186 936 1585 2468 Fragments arranged by size: 2468 1585 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2564 2654 1937 Size: 627 90 4594 Fragments arranged by size: 4594 627 90 Acc65I G'GTAC_C Cuts at: 2632 2632 Size: 5311 AclI AA'CG_TT Cuts at: 1563 2150 3438 3811 1563 Size: 587 1288 373 3063 Fragments arranged by size: 3063 1288 587 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2418 3374 4758 5026 1632 Size: 464 322 956 1384 268 1917 Fragments arranged by size: 1917 1384 956 464 322 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5114 AflIII A'CryG_T Cuts at: 1203 4210 5187 1203 Size: 3007 977 1327 Fragments arranged by size: 3007 1327 977 AgeI A'CCGG_T Cuts at: 4142 4142 Size: 5311 AhdI GACnn_n'nnGTC Cuts at: 4040 4040 Size: 5311 AleI CACnn'nnGTG Cuts at: 1166 2054 1166 Size: 888 4423 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2427 1184 Size: 1243 4068 AlwNI CAG_nnn'CTG Cuts at: 3054 4626 3054 Size: 1572 3739 ApaI G_GGCC'C Cuts at: 2492 2492 Size: 5311 ApaLI G'TGCA_C Cuts at: 3368 4524 3368 Size: 1156 4155 ApoI r'AATT_y Cuts at: 1493 2351 2620 2846 2998 5107 1493 Size: 858 269 226 152 2109 1697 Fragments arranged by size: 2109 1697 858 269 226 152 AseI AT'TA_AT Cuts at: 160 1032 3865 160 Size: 872 2833 1606 Fragments arranged by size: 2833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 AvaI C'yCGr_G Cuts at: 1870 1943 2636 1870 Size: 73 693 4545 Fragments arranged by size: 4545 693 73 BamHI G'GATC_C Cuts at: 2641 3008 2641 Size: 367 4944 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2632 4087 618 Size: 325 217 627 261 584 1455 1842 Fragments arranged by size: 1842 1455 627 584 325 261 217 BanII G_rGCy'C Cuts at: 1391 2492 2630 1391 Size: 1101 138 4072 Fragments arranged by size: 4072 1101 138 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2297 928 Size: 971 398 3942 Fragments arranged by size: 3942 971 398 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2134 2168 3502 3536 1903 Size: 34 197 34 1334 34 3678 Fragments arranged by size: 3678 1334 197 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3203 4413 3203 Size: 1210 4101 BclI T'GATC_A Cuts at: 1548 1548 Size: 5311 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3922 136 Size: 107 122 71 631 605 2250 1525 Fragments arranged by size: 2250 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2117 5306 2117 Size: 3189 2122 BlpI GC'TnA_GC Cuts at: 2728 2728 Size: 5311 Bme1580I G_kGCm'C Cuts at: 1790 2492 3372 4528 1790 Size: 702 880 1156 2573 Fragments arranged by size: 2573 1156 880 702 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5311 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3042 4000 442 Size: 416 53 83 399 24 1625 958 1753 Fragments arranged by size: 1753 1625 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5311 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2456 2456 Size: 5311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3971 1239 Size: 2732 2579 Bpu10I CC'TnA_GC Cuts at: 2064 2064 Size: 5311 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2106 2446 3436 4301 4599 2106 Size: 340 990 865 298 2818 Fragments arranged by size: 2818 990 865 340 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3974 882 Size: 617 2475 2219 Fragments arranged by size: 2475 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3102 493 Size: 711 1898 2702 Fragments arranged by size: 2702 1898 711 BsaBI GATnn'nnATC Cuts at: 2334 5305 2334 Size: 2971 2340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3118 3500 4135 275 Size: 53 83 186 936 1585 382 635 1451 Fragments arranged by size: 1585 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3744 4142 4416 4563 4974 3744 Size: 398 274 147 411 4081 Fragments arranged by size: 4081 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5086 Fragments arranged by size: 5086 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 1186 Size: 1285 4026 BseYI C'CCAG_C Cuts at: 2080 2485 4514 5010 2080 Size: 405 2029 496 2381 Fragments arranged by size: 2381 2029 496 405 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4738 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2761 3522 4550 1540 Size: 275 186 760 761 1028 2301 Fragments arranged by size: 2301 1028 761 760 275 186 BsiHKAI G_wGCw'C Cuts at: 2406 3372 3457 4528 2406 Size: 966 85 1071 3189 Fragments arranged by size: 3189 1071 966 85 BsmI GAATG_Cn' Cuts at: 2556 2822 2915 2556 Size: 266 93 4952 Fragments arranged by size: 4952 266 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2406 2492 2630 3372 3457 4528 1391 Size: 399 616 86 138 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 616 399 138 86 85 BspEI T'CCGG_A Cuts at: 4974 4974 Size: 5311 BspHI T'CATG_A Cuts at: 2442 3200 4930 2442 Size: 758 1730 2823 Fragments arranged by size: 2823 1730 758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2400 2666 844 Size: 1556 266 3489 Fragments arranged by size: 3489 1556 266 BsrBI CCG'CTC Cuts at: 1394 1823 3198 1394 Size: 429 1375 3507 Fragments arranged by size: 3507 1375 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3806 3980 65 Size: 1183 2558 174 1396 Fragments arranged by size: 2558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3955 4142 1700 Size: 110 2145 187 2869 Fragments arranged by size: 2869 2145 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5311 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5311 BssSI C'ACGA_G Cuts at: 3371 4383 3371 Size: 1012 4299 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5311 BstBI TT'CG_AA Cuts at: 4115 4115 Size: 5311 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5311 BstYI r'GATC_y Cuts at: 1972 2117 2641 3008 3393 3410 4862 4948 4960 Size: 145 524 367 385 17 1452 86 12 Cuts at: 4960 5306 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 524 385 367 346 145 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2522 513 Size: 552 939 518 3302 Fragments arranged by size: 3302 939 552 518 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4572 Fragments arranged by size: 4572 683 56 BtsI GCAGTG_nn' Cuts at: 2827 3621 3641 2827 Size: 794 20 4497 Fragments arranged by size: 4497 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5276 DraI TTT'AAA Cuts at: 2615 2615 Size: 5311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4318 809 Size: 507 3002 1802 Fragments arranged by size: 3002 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2758 3647 5276 Size: 54 1425 50 275 18 928 889 1629 Cuts at: 5276 8 Size: 43 Fragments arranged by size: 1629 1425 928 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2758 1537 Size: 275 946 4090 Fragments arranged by size: 4090 946 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 3241 1353 Size: 1041 847 3423 Fragments arranged by size: 3423 1041 847 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3898 4272 4418 387 Size: 1064 138 2309 374 146 1280 Fragments arranged by size: 2309 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2418 3374 3971 4758 5026 1239 Size: 393 464 322 956 597 787 268 1524 Fragments arranged by size: 1524 956 787 597 464 393 322 268 EcoNI CCTnn'n_nnAGG Cuts at: 2453 2453 Size: 5311 EcoO109I rG'GnC_Cy Cuts at: 2240 2489 2240 Size: 249 5062 EcoRI G'AATT_C Cuts at: 2620 2998 2620 Size: 378 4933 FspI TGC'GCA Cuts at: 2415 3817 2415 Size: 1402 3909 HaeII r_GCGC'y Cuts at: 1678 4458 1678 Size: 2780 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2082 2102 2114 2134 2500 Size: 32 135 32 510 20 12 20 366 Cuts at: 2500 2532 3952 3984 4026 4058 1373 Size: 32 1420 32 42 32 2626 Fragments arranged by size: 2626 1420 510 366 135 42 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2206 2565 2655 2901 669 Size: 988 281 268 359 90 246 3079 Fragments arranged by size: 3079 988 359 281 268 246 90 HindIII A'AGCT_T Cuts at: 748 2263 748 Size: 1515 3796 HpaI GTT'AAC Cuts at: 2901 2901 Size: 5311 KpnI G_GTAC'C Cuts at: 2636 2636 Size: 5311 MfeI C'AATT_G Cuts at: 2910 2910 Size: 5311 MluI A'CGCG_T Cuts at: 5187 5187 Size: 5311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4425 4609 1305 Size: 3120 184 2007 Fragments arranged by size: 3120 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3489 Fragments arranged by size: 3489 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 3269 3628 3787 518 Size: 648 888 1215 359 159 2042 Fragments arranged by size: 2042 1215 888 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2485 2727 3404 4552 4797 1606 Size: 879 242 677 1148 245 2120 Fragments arranged by size: 2120 1148 879 677 245 242 NcoI C'CATG_G Cuts at: 513 1065 2004 2522 513 Size: 552 939 518 3302 Fragments arranged by size: 3302 939 552 518 NdeI CA'TA_TG Cuts at: 387 387 Size: 5311 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5311 NotI GC'GGCC_GC Cuts at: 2758 2758 Size: 5311 NspI r_CATG'y Cuts at: 1132 1229 2669 4214 1132 Size: 97 1440 1545 2229 Fragments arranged by size: 2229 1545 1440 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5311 PciI A'CATG_T Cuts at: 4210 4210 Size: 5311 PflMI CCAn_nnn'nTGG Cuts at: 1108 2141 1108 Size: 1033 4278 PmeI GTTT'AAAC Cuts at: 2615 2615 Size: 5311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3380 3412 4893 4925 3380 Size: 32 1481 32 3766 Fragments arranged by size: 3766 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2240 2240 Size: 5311 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5311 PsiI TTA'TAA Cuts at: 2881 2881 Size: 5311 PspOMI G'GGCC_C Cuts at: 2488 2488 Size: 5311 PstI C_TGCA'G Cuts at: 830 2663 830 Size: 1833 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5311 PvuII CAG'CTG Cuts at: 2485 2485 Size: 5311 SalI G'TCGA_C Cuts at: 1936 2563 2653 1936 Size: 627 90 4594 Fragments arranged by size: 4594 627 90 SanDI GG'GwC_CC Cuts at: 2240 2240 Size: 5311 SapI GCTCTTCn'nnn_ Cuts at: 2394 2394 Size: 5311 SbfI CC_TGCA'GG Cuts at: 2663 2663 Size: 5311 ScaI AGT'ACT Cuts at: 3559 5271 3559 Size: 1712 3599 SfcI C'TryA_G Cuts at: 826 1045 2214 2659 3794 4475 4666 826 Size: 219 1169 445 1135 681 191 1471 Fragments arranged by size: 1471 1169 1135 681 445 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5311 SmaI CCC'GGG Cuts at: 1872 2638 1872 Size: 766 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2121 2425 3415 4316 4578 820 Size: 197 926 178 304 990 901 262 1553 Fragments arranged by size: 1553 990 926 901 304 262 197 178 SnaBI TAC'GTA Cuts at: 493 3102 493 Size: 2609 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5311 SphI G_CATG'C Cuts at: 1132 2669 1132 Size: 1537 3774 SspI AAT'ATT Cuts at: 5 52 3235 5 Size: 47 3183 2081 Fragments arranged by size: 3183 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5311 StyI C'CwwG_G Cuts at: 513 1065 2004 2235 2522 2750 513 Size: 552 939 231 287 228 3074 Fragments arranged by size: 3074 939 552 287 231 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3338 3355 3508 3693 4138 325 Size: 1255 1758 17 153 185 445 1498 Fragments arranged by size: 1758 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3557 5269 96 Size: 274 80 33 51 1443 1580 1712 138 Fragments arranged by size: 1712 1580 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5271 5303 5271 Size: 32 5279 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5311 XbaI T'CTAG_A Cuts at: 2371 2647 2371 Size: 276 5035 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5311 XmaI C'CCGG_G Cuts at: 1870 2636 1870 Size: 766 4545 XmnI GAAnn'nnTTC Cuts at: 3440 3440 Size: 5311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3119 276 Size: 53 83 186 936 1585 2468 Fragments arranged by size: 2468 1585 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI