Restriction map for
pFN21AB7557
(Linear) (Six-base) MAPSORT of: KIBB7557.seq Check: 1793 from: 1 to: 606
KIBB7557.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 559 606
Size: 559 47
AclI AA'CG_TT
Cuts at: 0 145 606
Size: 145 461
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 413 606
Size: 91 322 193
Fragments arranged by size:
322 193 91
AleI CACnn'nnGTG
Cuts at: 0 49 606
Size: 49 557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 422 606
Size: 422 184
ApaI G_GGCC'C
Cuts at: 0 487 606
Size: 487 119
ApoI r'AATT_y
Cuts at: 0 346 606
Size: 346 260
BanI G'GyrC_C
Cuts at: 0 43 606
Size: 43 563
BanII G_rGCy'C
Cuts at: 0 487 606
Size: 487 119
BbsI GAAGACnn'nnnn_
Cuts at: 0 292 606
Size: 292 314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 129 163 606
Size: 129 34 443
Fragments arranged by size:
443 129 34
BglII A'GATC_T
Cuts at: 0 112 606
Size: 112 494
Bme1580I G_kGCm'C
Cuts at: 0 487 606
Size: 487 119
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 451 606
Size: 451 155
Bpu10I CC'TnA_GC
Cuts at: 0 59 606
Size: 59 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 441 606
Size: 101 340 165
Fragments arranged by size:
340 165 101
BsaBI GATnn'nnATC
Cuts at: 0 329 606
Size: 329 277
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 606
Size: 466 140
BseYI C'CCAG_C
Cuts at: 0 75 480 606
Size: 75 405 126
Fragments arranged by size:
405 126 75
BsiHKAI G_wGCw'C
Cuts at: 0 401 606
Size: 401 205
BsmI GAATG_Cn'
Cuts at: 0 551 606
Size: 551 55
Bsp1286I G_dGCh'C
Cuts at: 0 401 487 606
Size: 401 86 119
Fragments arranged by size:
401 119 86
BspHI T'CATG_A
Cuts at: 0 437 606
Size: 437 169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 395 606
Size: 395 211
BstYI r'GATC_y
Cuts at: 0 112 606
Size: 112 494
BtgI C'CryG_G
Cuts at: 0 517 606
Size: 517 89
EarI CTCTTCn'nnn_
Cuts at: 0 389 606
Size: 389 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 413 606
Size: 91 322 193
Fragments arranged by size:
322 193 91
EcoNI CCTnn'n_nnAGG
Cuts at: 0 448 606
Size: 448 158
EcoO109I rG'GnC_Cy
Cuts at: 0 235 484 606
Size: 235 249 122
Fragments arranged by size:
249 235 122
FspI TGC'GCA
Cuts at: 0 410 606
Size: 410 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 77 97 109 129 495 527 606
Size: 77 20 12 20 366 32 79
Fragments arranged by size:
366 79 77 32 20 20 12
HincII GTy'rAC
Cuts at: 0 201 560 606
Size: 201 359 46
Fragments arranged by size:
359 201 46
HindIII A'AGCT_T
Cuts at: 0 258 606
Size: 258 348
MslI CAynn'nnrTG
Cuts at: 0 49 606
Size: 49 557
MspA1I CmG'CkG
Cuts at: 0 480 606
Size: 480 126
NcoI C'CATG_G
Cuts at: 0 517 606
Size: 517 89
PflMI CCAn_nnn'nTGG
Cuts at: 0 136 606
Size: 136 470
PpuMI rG'GwC_Cy
Cuts at: 0 235 606
Size: 235 371
PspOMI G'GGCC_C
Cuts at: 0 483 606
Size: 483 123
PvuII CAG'CTG
Cuts at: 0 480 606
Size: 480 126
SalI G'TCGA_C
Cuts at: 0 558 606
Size: 558 48
SanDI GG'GwC_CC
Cuts at: 0 235 606
Size: 235 371
SapI GCTCTTCn'nnn_
Cuts at: 0 389 606
Size: 389 217
SfcI C'TryA_G
Cuts at: 0 209 606
Size: 209 397
SmlI C'TyrA_G
Cuts at: 0 116 420 606
Size: 116 304 186
Fragments arranged by size:
304 186 116
StyI C'CwwG_G
Cuts at: 0 230 517 606
Size: 230 287 89
Fragments arranged by size:
287 230 89
XbaI T'CTAG_A
Cuts at: 0 366 606
Size: 366 240
Enzymes that do cut:
AccI AclI AcuI AleI AlfI ApaI ApoI BanI
BanII BbsI BcgI BglII Bme1580I BplI Bpu10I BpuEI
BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI
BstYI BtgI EarI Eco57MI EcoNI EcoO109I FspI Hin4I
HincII HindIII MslI MspA1I NcoI PflMI PpuMI PspOMI
PvuII SalI SanDI SapI SfcI SmlI StyI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspAI HaeII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI