(Circular) (Six-base) MAPSORT of: pFN21AB7550.seq Check: 4293 from: 1 to: 5506 pFN21AB7550 5506 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2369 2369 Size: 5506 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2433 3316 278 Size: 53 83 186 936 897 883 2468 Fragments arranged by size: 2468 936 897 883 186 83 53 AccI GT'mk_AC Cuts at: 1937 2849 1937 Size: 912 4594 Acc65I G'GTAC_C Cuts at: 2827 2827 Size: 5506 AclI AA'CG_TT Cuts at: 1563 3633 4006 1563 Size: 2070 373 3063 Fragments arranged by size: 3063 2070 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2277 2720 3569 4953 5221 1632 Size: 645 443 849 1384 268 1917 Fragments arranged by size: 1917 1384 849 645 443 268 AfeI AGC'GCT Cuts at: 2561 2561 Size: 5506 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5309 AflIII A'CryG_T Cuts at: 1203 2494 2613 2643 4405 5382 1203 Size: 1291 119 30 1762 977 1327 Fragments arranged by size: 1762 1327 1291 977 119 30 AgeI A'CCGG_T Cuts at: 4337 4337 Size: 5506 AhdI GACnn_n'nnGTC Cuts at: 4235 4235 Size: 5506 AleI CACnn'nnGTG Cuts at: 1166 2513 1166 Size: 1347 4159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5506 AlwNI CAG_nnn'CTG Cuts at: 3249 4821 3249 Size: 1572 3934 ApaI G_GGCC'C Cuts at: 2232 2232 Size: 5506 ApaLI G'TGCA_C Cuts at: 3563 4719 3563 Size: 1156 4350 ApoI r'AATT_y Cuts at: 1493 2815 3041 3193 5302 1493 Size: 1322 226 152 2109 1697 Fragments arranged by size: 2109 1697 1322 226 152 AseI AT'TA_AT Cuts at: 160 1032 4060 160 Size: 872 3028 1606 Fragments arranged by size: 3028 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2831 1870 Size: 73 282 606 4545 Fragments arranged by size: 4545 606 282 73 BamHI G'GATC_C Cuts at: 2836 3203 2836 Size: 367 5139 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2439 2827 4282 618 Size: 325 217 627 652 388 1455 1842 Fragments arranged by size: 1842 1455 652 627 388 325 217 BanII G_rGCy'C Cuts at: 1391 2070 2232 2825 1391 Size: 679 162 593 4072 Fragments arranged by size: 4072 679 593 162 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2265 928 Size: 971 366 4169 Fragments arranged by size: 4169 971 366 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3697 3731 1903 Size: 34 50 34 1676 34 3678 Fragments arranged by size: 3678 1676 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3398 4608 3398 Size: 1210 4296 BclI T'GATC_A Cuts at: 1548 1548 Size: 5506 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2682 4117 136 Size: 107 122 71 631 605 1010 1435 1525 Fragments arranged by size: 1525 1435 1010 631 605 122 107 71 BglII A'GATC_T Cuts at: 2091 5501 2091 Size: 3410 2096 BlpI GC'TnA_GC Cuts at: 2923 2923 Size: 5506 Bme1580I G_kGCm'C Cuts at: 1790 2232 3567 4723 1790 Size: 442 1335 1156 2573 Fragments arranged by size: 2573 1335 1156 442 BmgBI CAC'GTC Cuts at: 1318 2497 2689 1318 Size: 1179 192 4135 Fragments arranged by size: 4135 1179 192 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2337 3237 4195 Size: 416 53 83 399 24 920 900 958 Cuts at: 4195 442 Size: 1753 Fragments arranged by size: 1753 958 920 900 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5506 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 2591 4166 1239 Size: 841 511 1575 2579 Fragments arranged by size: 2579 1575 841 511 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2471 2567 3631 4496 4794 2471 Size: 96 1064 865 298 3183 Fragments arranged by size: 3183 1064 865 298 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2286 2343 4169 882 Size: 617 787 57 1826 2219 Fragments arranged by size: 2219 1826 787 617 57 BsaAI yAC'GTr Cuts at: 493 1204 2046 2098 2451 2644 3297 493 Size: 711 842 52 353 193 653 2702 Fragments arranged by size: 2702 842 711 653 353 193 52 BsaBI GATnn'nnATC Cuts at: 5500 5500 Size: 5506 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2430 3313 3695 4330 Size: 53 83 186 936 897 883 382 635 Cuts at: 4330 275 Size: 1451 Fragments arranged by size: 1451 936 897 883 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3939 4337 4611 4758 5169 3939 Size: 398 274 147 411 4276 Fragments arranged by size: 4276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5281 Fragments arranged by size: 5281 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2666 1186 Size: 907 573 4026 Fragments arranged by size: 4026 907 573 BseYI C'CCAG_C Cuts at: 2244 4709 5205 2244 Size: 2465 496 2545 Fragments arranged by size: 2545 2465 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2431 2551 2621 2647 2678 1163 Size: 573 695 120 70 26 31 3991 Fragments arranged by size: 3991 695 573 120 70 31 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2956 3717 4745 1540 Size: 275 186 955 761 1028 2301 Fragments arranged by size: 2301 1028 955 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 2709 3567 3652 4723 2070 Size: 639 858 85 1071 2853 Fragments arranged by size: 2853 1071 858 639 85 BsmI GAATG_Cn' Cuts at: 3017 3110 3017 Size: 93 5413 BsmBI CGTCTCn'nnnn_ Cuts at: 2044 2044 Size: 5506 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2232 2709 2825 3567 3652 4723 Size: 399 280 162 477 116 742 85 1071 Cuts at: 4723 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 477 399 280 162 116 85 BspEI T'CCGG_A Cuts at: 5169 5169 Size: 5506 BspHI T'CATG_A Cuts at: 3395 5125 3395 Size: 1730 3776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2369 2861 844 Size: 1525 492 3489 Fragments arranged by size: 3489 1525 492 BsrBI CCG'CTC Cuts at: 1394 1823 2626 3393 1394 Size: 429 803 767 3507 Fragments arranged by size: 3507 803 767 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4001 4175 65 Size: 1183 2753 174 1396 Fragments arranged by size: 2753 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4150 4337 1700 Size: 110 2340 187 2869 Fragments arranged by size: 2869 2340 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5506 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5506 BssSI C'ACGA_G Cuts at: 3566 4578 3566 Size: 1012 4494 BstAPI GCAn_nnn'nTGC Cuts at: 794 2454 794 Size: 1660 3846 BstBI TT'CG_AA Cuts at: 4310 4310 Size: 5506 BstEII G'GTnAC_C Cuts at: 1184 2323 1184 Size: 1139 4367 BstXI CCAn_nnnn'nTGG Cuts at: 2749 2749 Size: 5506 BstYI r'GATC_y Cuts at: 1972 2091 2589 2836 3203 3588 3605 5057 5143 Size: 119 498 247 367 385 17 1452 86 Cuts at: 5143 5155 5501 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 498 385 367 346 247 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2296 2593 2754 513 Size: 552 1231 297 161 3265 Fragments arranged by size: 3265 1231 552 297 161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4767 Fragments arranged by size: 4767 683 56 BtsI GCAGTG_nn' Cuts at: 3022 3816 3836 3022 Size: 794 20 4692 Fragments arranged by size: 4692 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5506 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5471 DraI TTT'AAA Cuts at: 2810 2810 Size: 5506 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4513 809 Size: 507 3197 1802 Fragments arranged by size: 3197 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2751 2953 Size: 54 1425 50 275 18 327 594 202 Cuts at: 2953 3842 5471 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 594 327 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2953 1537 Size: 275 1141 4090 Fragments arranged by size: 4090 1141 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3436 1353 Size: 2083 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4093 4467 4613 387 Size: 1064 138 2504 374 146 1280 Fragments arranged by size: 2504 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2068 2068 Size: 5506 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2277 2591 2720 3569 4166 4953 Size: 393 448 197 314 129 849 597 787 Cuts at: 4953 5221 1239 Size: 268 1524 Fragments arranged by size: 1524 849 787 597 448 393 314 268 197 129 EcoNI CCTnn'n_nnAGG Cuts at: 2786 2786 Size: 5506 EcoO109I rG'GnC_Cy Cuts at: 2229 2229 Size: 5506 EcoRI G'AATT_C Cuts at: 2815 3193 2815 Size: 378 5128 FspI TGC'GCA Cuts at: 2309 2732 4012 2309 Size: 423 1280 3803 Fragments arranged by size: 3803 1280 423 HaeII r_GCGC'y Cuts at: 1678 2563 4653 1678 Size: 885 2090 2531 Fragments arranged by size: 2531 2090 885 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373 Size: 32 135 32 2575 32 42 32 2626 Fragments arranged by size: 2626 2575 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3096 669 Size: 988 281 912 246 3079 Fragments arranged by size: 3079 988 912 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5506 HpaI GTT'AAC Cuts at: 3096 3096 Size: 5506 KpnI G_GTAC'C Cuts at: 2831 2831 Size: 5506 MfeI C'AATT_G Cuts at: 3105 3105 Size: 5506 MluI A'CGCG_T Cuts at: 2613 5382 2613 Size: 2769 2737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4620 4804 1305 Size: 3315 184 2007 Fragments arranged by size: 3315 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3684 Fragments arranged by size: 3684 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2513 3464 3823 3982 518 Size: 648 1149 198 951 359 159 2042 Fragments arranged by size: 2042 1149 951 648 359 198 159 MspA1I CmG'CkG Cuts at: 1606 2179 2248 2725 2922 3599 4747 4992 1606 Size: 573 69 477 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 573 477 245 197 69 NcoI C'CATG_G Cuts at: 513 1065 2296 2593 513 Size: 552 1231 297 3426 Fragments arranged by size: 3426 1231 552 297 NdeI CA'TA_TG Cuts at: 387 387 Size: 5506 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5506 NotI GC'GGCC_GC Cuts at: 2953 2953 Size: 5506 NspI r_CATG'y Cuts at: 1132 1229 2401 2864 4409 1132 Size: 97 1172 463 1545 2229 Fragments arranged by size: 2229 1545 1172 463 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5506 PciI A'CATG_T Cuts at: 4405 4405 Size: 5506 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5506 PmeI GTTT'AAAC Cuts at: 2810 2810 Size: 5506 PmlI CAC'GTG Cuts at: 2046 2046 Size: 5506 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3575 3607 5088 5120 3575 Size: 32 1481 32 3961 Fragments arranged by size: 3961 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5506 PsiI TTA'TAA Cuts at: 3076 3076 Size: 5506 PspOMI G'GGCC_C Cuts at: 2228 2228 Size: 5506 PstI C_TGCA'G Cuts at: 830 2790 2858 830 Size: 1960 68 3478 Fragments arranged by size: 3478 1960 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5506 PvuII CAG'CTG Cuts at: 2248 2248 Size: 5506 SacI G_AGCT'C Cuts at: 2070 2070 Size: 5506 SalI G'TCGA_C Cuts at: 1936 2848 1936 Size: 912 4594 SbfI CC_TGCA'GG Cuts at: 2858 2858 Size: 5506 ScaI AGT'ACT Cuts at: 3754 5466 3754 Size: 1712 3794 SexAI A'CCwGG_T Cuts at: 2802 2802 Size: 5506 SfcI C'TryA_G Cuts at: 826 1045 2212 2762 2786 2854 3989 4670 4861 Size: 219 1167 550 24 68 1135 681 191 Cuts at: 4861 826 Size: 1471 Fragments arranged by size: 1471 1167 1135 681 550 219 191 68 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5506 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5506 SmaI CCC'GGG Cuts at: 1872 2227 2833 1872 Size: 355 606 4545 Fragments arranged by size: 4545 606 355 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 2582 3610 4511 4773 820 Size: 197 926 543 96 1028 901 262 1553 Fragments arranged by size: 1553 1028 926 901 543 262 197 96 SnaBI TAC'GTA Cuts at: 493 3297 493 Size: 2804 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5506 SphI G_CATG'C Cuts at: 1132 2401 2864 1132 Size: 1269 463 3774 Fragments arranged by size: 3774 1269 463 SspI AAT'ATT Cuts at: 5 52 3430 5 Size: 47 3378 2081 Fragments arranged by size: 3378 2081 47 StuI AGG'CCT Cuts at: 1517 2381 2549 1517 Size: 864 168 4474 Fragments arranged by size: 4474 864 168 StyI C'CwwG_G Cuts at: 513 1065 2296 2329 2593 2945 513 Size: 552 1231 33 264 352 3074 Fragments arranged by size: 3074 1231 552 352 264 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3533 3550 3703 3888 4333 325 Size: 1255 1953 17 153 185 445 1498 Fragments arranged by size: 1953 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3752 5464 96 Size: 274 80 33 51 1443 1775 1712 138 Fragments arranged by size: 1775 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5466 5498 5466 Size: 32 5474 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5506 XbaI T'CTAG_A Cuts at: 2842 2842 Size: 5506 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5506 XmaI C'CCGG_G Cuts at: 1870 2225 2831 1870 Size: 355 606 4545 Fragments arranged by size: 4545 606 355 XmnI GAAnn'nnTTC Cuts at: 2381 2465 3635 2381 Size: 84 1170 4252 Fragments arranged by size: 4252 1170 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2431 3314 276 Size: 53 83 186 936 897 883 2468 Fragments arranged by size: 2468 936 897 883 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI