(Circular) (Six-base) MAPSORT of: pFN21AB7546.seq Check: 6726 from: 1 to: 5497 pFN21AB7546 5497 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2130 2130 Size: 5497 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3307 278 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2840 1937 Size: 903 4594 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2337 3560 4944 5212 1632 Size: 705 1223 1384 268 1917 Fragments arranged by size: 1917 1384 1223 705 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 4396 5373 1203 Size: 3193 977 1327 Fragments arranged by size: 3193 1327 977 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5497 AlwNI CAG_nnn'CTG Cuts at: 3240 4812 3240 Size: 1572 3925 ApaI G_GGCC'C Cuts at: 2680 2680 Size: 5497 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2806 3032 3184 5293 1493 Size: 1313 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2822 1870 Size: 73 879 4545 Fragments arranged by size: 4545 879 73 BamHI G'GATC_C Cuts at: 2827 3194 2827 Size: 367 5130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2200 2504 2818 4273 618 Size: 325 217 627 413 304 314 1455 1842 Fragments arranged by size: 1842 1455 627 413 325 314 304 217 BanII G_rGCy'C Cuts at: 1391 2680 2816 1391 Size: 1289 136 4072 Fragments arranged by size: 4072 1289 136 BbeI G_GCGC'C Cuts at: 2204 2204 Size: 5497 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4526 BbvCI CC'TCA_GC Cuts at: 2495 2495 Size: 5497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2363 2397 3688 3722 1903 Size: 34 50 34 342 34 1291 34 3678 Fragments arranged by size: 3678 1291 342 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3389 4599 3389 Size: 1210 4287 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4108 136 Size: 107 122 71 631 605 2436 1525 Fragments arranged by size: 2436 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5492 5492 Size: 5497 BlpI GC'TnA_GC Cuts at: 2914 2914 Size: 5497 Bme1580I G_kGCm'C Cuts at: 1790 2412 2509 2680 3558 4714 1790 Size: 622 97 171 878 1156 2573 Fragments arranged by size: 2573 1156 878 622 171 97 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5497 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3228 4186 442 Size: 416 53 83 399 24 1811 958 1753 Fragments arranged by size: 1811 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2287 2287 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2432 2669 4157 1239 Size: 986 207 237 1488 2579 Fragments arranged by size: 2579 1488 986 237 207 Bpu10I CC'TnA_GC Cuts at: 2495 2782 2495 Size: 287 5210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2495 3622 4487 4785 2495 Size: 1127 865 298 3207 Fragments arranged by size: 3207 1127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4160 882 Size: 617 2661 2219 Fragments arranged by size: 2661 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 2519 5491 2519 Size: 2972 2525 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2201 3304 3686 4321 Size: 53 83 186 936 668 1103 382 635 Cuts at: 4321 275 Size: 1451 Fragments arranged by size: 1451 1103 936 668 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2143 2601 3930 4328 4602 4749 5160 2143 Size: 458 1329 398 274 147 411 2480 Fragments arranged by size: 2480 1329 458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5272 Fragments arranged by size: 5272 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2335 1186 Size: 1149 4348 BseYI C'CCAG_C Cuts at: 2211 2304 2312 2424 4700 5196 2211 Size: 93 8 112 2276 496 2512 Fragments arranged by size: 2512 2276 496 112 93 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4924 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2947 3708 4736 1540 Size: 275 186 946 761 1028 2301 Fragments arranged by size: 2301 1028 946 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2731 3558 3643 4714 2731 Size: 827 85 1071 3514 Fragments arranged by size: 3514 1071 827 85 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2412 2509 2680 2731 2816 3558 3643 Size: 399 622 97 171 51 85 742 85 Cuts at: 3643 4714 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 622 399 171 97 85 85 51 BspEI T'CCGG_A Cuts at: 2143 2601 5160 2143 Size: 458 2559 2480 Fragments arranged by size: 2559 2480 458 BspHI T'CATG_A Cuts at: 3386 5116 3386 Size: 1730 3767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 2564 2679 2852 844 Size: 1286 434 115 173 3489 Fragments arranged by size: 3489 1286 434 173 115 BsrBI CCG'CTC Cuts at: 1394 1823 2664 3384 1394 Size: 429 841 720 3507 Fragments arranged by size: 3507 841 720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2326 4141 4328 1700 Size: 110 516 1815 187 2869 Fragments arranged by size: 2869 1815 516 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 2478 794 Size: 1684 3813 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstXI CCAn_nnnn'nTGG Cuts at: 2223 2419 2223 Size: 196 5301 BstYI r'GATC_y Cuts at: 1972 2148 2443 2827 3194 3579 3596 5048 5134 Size: 176 295 384 367 385 17 1452 86 Cuts at: 5134 5146 5492 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 384 367 346 295 176 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4945 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4758 Fragments arranged by size: 4758 683 56 BtsI GCAGTG_nn' Cuts at: 2638 3013 3807 3827 2638 Size: 375 794 20 4308 Fragments arranged by size: 4308 794 375 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2275 4504 809 Size: 507 959 2229 1802 Fragments arranged by size: 2229 1802 959 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2944 3833 5462 Size: 54 1425 50 275 18 1114 889 1629 Cuts at: 5462 8 Size: 43 Fragments arranged by size: 1629 1425 1114 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2944 1537 Size: 275 1132 4090 Fragments arranged by size: 4090 1132 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2365 3427 1353 Size: 1012 1062 3423 Fragments arranged by size: 3423 1062 1012 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4084 4458 4604 387 Size: 1064 138 2495 374 146 1280 Fragments arranged by size: 2495 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2337 2432 2669 3560 4157 4944 Size: 393 593 112 95 237 891 597 787 Cuts at: 4944 5212 1239 Size: 268 1524 Fragments arranged by size: 1524 891 787 597 593 393 268 237 112 95 EcoNI CCTnn'n_nnAGG Cuts at: 2572 2572 Size: 5497 EcoO109I rG'GnC_Cy Cuts at: 2577 2577 Size: 5497 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 2204 4644 1678 Size: 526 2440 2531 Fragments arranged by size: 2531 2440 526 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4138 4170 4212 4244 1373 Size: 32 135 32 2566 32 42 32 2626 Fragments arranged by size: 2626 2566 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2841 3087 669 Size: 988 281 903 246 3079 Fragments arranged by size: 3079 988 903 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KasI G'GCGC_C Cuts at: 2200 2200 Size: 5497 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 2217 3096 2217 Size: 879 4618 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 2691 4611 4795 1305 Size: 756 630 1920 184 2007 Fragments arranged by size: 2007 1920 756 630 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3675 Fragments arranged by size: 3675 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2697 3455 3814 3973 518 Size: 648 1531 758 359 159 2042 Fragments arranged by size: 2042 1531 758 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2419 2913 3590 4738 4983 1606 Size: 813 494 677 1148 245 2120 Fragments arranged by size: 2120 1148 813 677 494 245 NarI GG'CG_CC Cuts at: 2201 2201 Size: 5497 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4945 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2855 4400 1132 Size: 97 1626 1545 2229 Fragments arranged by size: 2229 1626 1545 97 PasI CC'CwG_GG Cuts at: 1348 2253 1348 Size: 905 4592 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 2125 1108 Size: 1017 4480 PfoI T'CCnGG_A Cuts at: 2241 2241 Size: 5497 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PspOMI G'GGCC_C Cuts at: 2676 2676 Size: 5497 PstI C_TGCA'G Cuts at: 830 2073 2645 2849 830 Size: 1243 572 204 3478 Fragments arranged by size: 3478 1243 572 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 PvuII CAG'CTG Cuts at: 2419 2419 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SfcI C'TryA_G Cuts at: 826 1045 2059 2069 2641 2845 3980 4661 4852 Size: 219 1014 10 572 204 1135 681 191 Cuts at: 4852 826 Size: 1471 Fragments arranged by size: 1471 1135 1014 681 572 219 204 191 10 SfoI GGC'GCC Cuts at: 2202 2202 Size: 5497 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2824 1872 Size: 952 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 3601 4502 4764 820 Size: 197 926 567 1091 901 262 1553 Fragments arranged by size: 1553 1091 926 901 567 262 197 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2855 1132 Size: 1723 3774 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 2378 2462 2567 1517 Size: 861 84 105 4447 Fragments arranged by size: 4447 861 105 84 StyI C'CwwG_G Cuts at: 513 1065 2364 2439 2936 513 Size: 552 1299 75 497 3074 Fragments arranged by size: 3074 1299 552 497 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2492 3524 3541 3694 3879 4324 325 Size: 1255 912 1032 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1032 912 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 5455 96 Size: 274 80 33 51 1443 1766 1712 138 Fragments arranged by size: 1766 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2756 2788 5457 5489 2756 Size: 32 2669 32 2764 Fragments arranged by size: 2764 2669 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2192 1316 Size: 876 4621 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2181 2181 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5497 XmaI C'CCGG_G Cuts at: 1870 2822 1870 Size: 952 4545 XmnI GAAnn'nnTTC Cuts at: 2567 2624 3626 2567 Size: 57 1002 4438 Fragments arranged by size: 4438 1002 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3305 276 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI