Restriction map for
pFN21AB7545
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BsrI BssHII BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI CelII ClaI CpoI DraI
DraII DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HphI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MroI MunI NarI NciI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SfcI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI TspRI TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AlwI 198 GATCC GGATC(4/5)
AocI 148 CCTGAGG CC^T[ACGT]AGG
AseI 492 ATTAAT AT^TAAT
AsnI 492 ATTAAT AT^TAAT
AspHI 119 GAGCTC G[AT]GC[AT]^C
AspI 529 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 283 GGTCC G^G[AT]CC
BalI 111 TGGCCA TGG^CCA
BanII 119 GAGCTC G[GA]GC[CT]^C
BbsI 430 GAAGAC GAAGAC(2/6)
BbvCI 73 CCTCAGC CCTCAGC(-5/-2)
BpmI 122 CTCCAG CTGGAG(16/14)
BpuAI 430 GAAGAC GAAGAC(2/6)
BsaAI 512 TACGTG [CT]AC^GT[GA]
BsiHKAI 119 GAGCTC G[AT]GC[AT]^C
BspMI 339 GCAGGT ACCTGC(4/8)
BsrFI 261 GCCGGC [GA]^CCGG[CT]
BssSI 166 CACGAG CACGAG(-5/-1)
Bst1107I 46 GTATAC GTA^TAC
BstZ17I 46 GTATAC GTA^TAC
Bsu36I 148 CCTGAGG CC^T[ACGT]AGG
Cfr10I 261 GCCGGC [GA]^CCGG[CT]
DpnII 198 GATC ^GATC
DrdI 526 GACGACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 275 CCATGG C^C[GA][CT]GG
Eco81I 148 CCTGAGG CC^T[ACGT]AGG
MaeII 513 ACGT A^CGT
MaeIII 424 GTGAC ^GT[ACGT]AC
MboI 198 GATC ^GATC
MboII 430 GAAGA GAAGA(8/7)
MluNI 111 TGGCCA TGG^CCA
MscI 111 TGGCCA TGG^CCA
MslI 540 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 76 CAGCTG C[AC]G^C[GT]G
NaeI 261 GCCGGC GCC^GGC
NcoI 275 CCATGG C^CATGG
NdeII 198 GATC ^GATC
NgoMIV 261 GCCGGC G^CCGGC
NspI 299 ACATGC [GA]CATG^[CT]
PflFI 529 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 492 ATTAAT AT^TAAT
PvuII 76 CAGCTG CAG^CTG
SacI 119 GAGCTC GAGCT^C
Sau3AI 198 GATC ^GATC
SexAI 50 ACCAGGT A^CC[AT]GGT
SfaNI 88 GCATC GCATC(5/9)
StuI 126 AGGCCT AGG^CCT
Tsp45I 424 GTGAC ^GT[GC]AC
Tsp509I 491 AATT ^AATT
Tth111I 529 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 68 CCACACCTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 46 GTATAC GT^[AC][GT]AC
535 GTCTAC
AccII 5 CGCG CG^CG
184 CGCG
AvaI 202 CCCGAG C^[CT]CG[GA]G
292 CCCGAG
BglI 104 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
265 GCCCAGGCGGC
BmyI 119 GAGCTC G[AGT]GC[ACT]^C
388 GTGCCC
BseRI 256 GAGGAG GAGGAG(10/8)
346 GAGGAG
BsoBI 202 CCCGAG C^[CT]CG[GA]G
292 CCCGAG
Bsp1286I 119 GAGCTC G[AGT]GC[ACT]^C
388 GTGCCC
BstUI 5 CGCG CG^CG
184 CGCG
Cac8I 261 GCCGGC GC[ACGT]^[ACGT]GC
498 GCTCGC
EaeI 111 TGGCCA [CT]^GGCC[GA]
272 CGGCCA
EcoT14I 114 CCAAGG C^C[AT][AT]GG
275 CCATGG
FauI 249 CCCGC CCCGC(4/6)
441 GCGGG
HapII 253 CCGG C^CGG
262 CCGG
HpaII 253 CCGG C^CGG
262 CCGG
MseI 452 TTAA T^TAA
493 TTAA
MspI 253 CCGG C^CGG
262 CCGG
MvnI 5 CGCG CG^CG
184 CGCG
SfiI 103 GGCCATGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
264 GGCCCAGGCGGCC
StyI 114 CCAAGG C^C[AT][AT]GG
275 CCATGG
Tru9I 452 TTAA T^TAA
493 TTAA
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstNI CfoI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HhaI
HinP1I ItaI MnlI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI TseI