(Circular) (Six-base) MAPSORT of: pFN21AB7545.seq Check: 3448 from: 1 to: 5257 pFN21AB7545 5257 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2335 2335 Size: 5257 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3067 278 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2052 2541 2600 1937 Size: 115 489 59 4594 Fragments arranged by size: 4594 489 115 59 Acc65I G'GTAC_C Cuts at: 2578 2578 Size: 5257 AclI AA'CG_TT Cuts at: 1563 3384 3757 1563 Size: 1821 373 3063 Fragments arranged by size: 3063 1821 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2454 3320 4704 4972 1632 Size: 822 866 1384 268 1917 Fragments arranged by size: 1917 1384 866 822 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5060 AflIII A'CryG_T Cuts at: 1203 4156 5133 1203 Size: 2953 977 1327 Fragments arranged by size: 2953 1327 977 AgeI A'CCGG_T Cuts at: 4088 4088 Size: 5257 AhdI GACnn_n'nnGTC Cuts at: 3986 3986 Size: 5257 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5257 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5257 AlwNI CAG_nnn'CTG Cuts at: 3000 4572 3000 Size: 1572 3685 ApaLI G'TGCA_C Cuts at: 3314 4470 3314 Size: 1156 4101 ApoI r'AATT_y Cuts at: 1493 2566 2792 2944 5053 1493 Size: 1073 226 152 2109 1697 Fragments arranged by size: 2109 1697 1073 226 152 AseI AT'TA_AT Cuts at: 160 1032 2498 3811 160 Size: 872 1466 1313 1606 Fragments arranged by size: 1606 1466 1313 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 AvaI C'yCGr_G Cuts at: 1870 1943 2207 2297 2582 1870 Size: 73 264 90 285 4545 Fragments arranged by size: 4545 285 264 90 73 BamHI G'GATC_C Cuts at: 2587 2954 2587 Size: 367 4890 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2578 4033 618 Size: 325 217 627 791 1455 1842 Fragments arranged by size: 1842 1455 791 627 325 217 BanII G_rGCy'C Cuts at: 1391 2128 2576 1391 Size: 737 448 4072 Fragments arranged by size: 4072 737 448 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2442 928 Size: 971 543 3743 Fragments arranged by size: 3743 971 543 BbvCI CC'TCA_GC Cuts at: 2079 2079 Size: 5257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3448 3482 1903 Size: 34 1511 34 3678 Fragments arranged by size: 3678 1511 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3149 4359 3149 Size: 1210 4047 BclI T'GATC_A Cuts at: 1548 1548 Size: 5257 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2115 2276 3868 Size: 107 122 71 631 605 443 161 1592 Cuts at: 3868 136 Size: 1525 Fragments arranged by size: 1592 1525 631 605 443 161 122 107 71 BglII A'GATC_T Cuts at: 5252 5252 Size: 5257 BlpI GC'TnA_GC Cuts at: 2674 2674 Size: 5257 Bme1580I G_kGCm'C Cuts at: 1790 2397 3318 4474 1790 Size: 607 921 1156 2573 Fragments arranged by size: 2573 1156 921 607 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5257 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2988 3946 442 Size: 416 53 83 399 24 1571 958 1753 Fragments arranged by size: 1753 1571 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5257 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2112 3917 1239 Size: 873 1805 2579 Fragments arranged by size: 2579 1805 873 Bpu10I CC'TnA_GC Cuts at: 2079 2079 Size: 5257 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3382 4247 4545 3382 Size: 865 298 4094 Fragments arranged by size: 4094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3920 882 Size: 617 2421 2219 Fragments arranged by size: 2421 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2519 3048 493 Size: 711 1315 529 2702 Fragments arranged by size: 2702 1315 711 529 BsaBI GATnn'nnATC Cuts at: 5251 5251 Size: 5257 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3064 3446 4081 275 Size: 53 83 186 936 1531 382 635 1451 Fragments arranged by size: 1531 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3690 4088 4362 4509 4920 3690 Size: 398 274 147 411 4027 Fragments arranged by size: 4027 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5032 Fragments arranged by size: 5032 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2366 1186 Size: 1090 90 4077 Fragments arranged by size: 4077 1090 90 BseYI C'CCAG_C Cuts at: 2083 4460 4956 2083 Size: 2377 496 2384 Fragments arranged by size: 2384 2377 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4684 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2707 3468 4496 1540 Size: 275 186 706 761 1028 2301 Fragments arranged by size: 2301 1028 761 706 275 186 BsiHKAI G_wGCw'C Cuts at: 2128 3318 3403 4474 2128 Size: 1190 85 1071 2911 Fragments arranged by size: 2911 1190 1071 85 BsmI GAATG_Cn' Cuts at: 2768 2861 2768 Size: 93 5164 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2397 2576 3318 3403 4474 1391 Size: 399 338 269 179 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 338 269 179 85 BspEI T'CCGG_A Cuts at: 4920 4920 Size: 5257 BspHI T'CATG_A Cuts at: 3146 4876 3146 Size: 1730 3527 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2335 2612 844 Size: 1491 277 3489 Fragments arranged by size: 3489 1491 277 BsrBI CCG'CTC Cuts at: 1394 1823 3144 1394 Size: 429 1321 3507 Fragments arranged by size: 3507 1321 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3752 3926 65 Size: 1183 2504 174 1396 Fragments arranged by size: 2504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2266 3901 4088 1700 Size: 110 456 1635 187 2869 Fragments arranged by size: 2869 1635 456 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5257 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5257 BssSI C'ACGA_G Cuts at: 2171 3317 4329 2171 Size: 1146 1012 3099 Fragments arranged by size: 3099 1146 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5257 BstBI TT'CG_AA Cuts at: 4061 4061 Size: 5257 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5257 BstYI r'GATC_y Cuts at: 1972 2587 2954 3339 3356 4808 4894 4906 5252 Size: 615 367 385 17 1452 86 12 346 Cuts at: 5252 1972 Size: 1977 Fragments arranged by size: 1977 1452 615 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2053 2053 Size: 5257 Bsu36I CC'TnA_GG Cuts at: 2154 2154 Size: 5257 BtgI C'CryG_G Cuts at: 513 1065 2004 2280 513 Size: 552 939 276 3490 Fragments arranged by size: 3490 939 552 276 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2205 487 Size: 683 56 979 3539 Fragments arranged by size: 3539 979 683 56 BtsI GCAGTG_nn' Cuts at: 2773 3567 3587 2773 Size: 794 20 4443 Fragments arranged by size: 4443 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5257 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5222 DraI TTT'AAA Cuts at: 2561 2561 Size: 5257 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2537 4264 809 Size: 507 1221 1727 1802 Fragments arranged by size: 1802 1727 1221 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2277 2704 Size: 54 1425 50 275 18 286 161 427 Cuts at: 2704 3593 5222 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 427 286 275 161 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2704 1537 Size: 275 892 4090 Fragments arranged by size: 4090 892 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3187 1353 Size: 1834 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3844 4218 4364 387 Size: 1064 138 2255 374 146 1280 Fragments arranged by size: 2255 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2126 2126 Size: 5257 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2454 3320 3917 4704 4972 1239 Size: 393 480 342 866 597 787 268 1524 Fragments arranged by size: 1524 866 787 597 480 393 342 268 EcoRI G'AATT_C Cuts at: 2566 2944 2566 Size: 378 4879 FspI TGC'GCA Cuts at: 3763 3763 Size: 5257 HaeII r_GCGC'y Cuts at: 1678 4404 1678 Size: 2726 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3898 3930 3972 4004 1373 Size: 32 135 32 2326 32 42 32 2626 Fragments arranged by size: 2626 2326 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2601 2847 669 Size: 988 281 663 246 3079 Fragments arranged by size: 3079 988 663 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5257 HpaI GTT'AAC Cuts at: 2847 2847 Size: 5257 KpnI G_GTAC'C Cuts at: 2582 2582 Size: 5257 MfeI C'AATT_G Cuts at: 2856 2856 Size: 5257 MluI A'CGCG_T Cuts at: 5133 5133 Size: 5257 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2395 4371 4555 1305 Size: 1090 1976 184 2007 Fragments arranged by size: 2007 1976 1090 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2118 10 Size: 54 1425 343 286 3149 Fragments arranged by size: 3149 1425 343 286 54 MslI CAynn'nnrTG Cuts at: 518 1166 2549 3215 3574 3733 518 Size: 648 1383 666 359 159 2042 Fragments arranged by size: 2042 1383 666 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2673 3350 4498 4743 1606 Size: 477 590 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 590 477 245 NaeI GCC'GGC Cuts at: 2268 2268 Size: 5257 NcoI C'CATG_G Cuts at: 513 1065 2004 2280 513 Size: 552 939 276 3490 Fragments arranged by size: 3490 939 552 276 NdeI CA'TA_TG Cuts at: 387 387 Size: 5257 NgoMIV G'CCGG_C Cuts at: 2266 2266 Size: 5257 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5257 NotI GC'GGCC_GC Cuts at: 2704 2704 Size: 5257 NspI r_CATG'y Cuts at: 1132 1229 2308 2615 4160 1132 Size: 97 1079 307 1545 2229 Fragments arranged by size: 2229 1545 1079 307 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5257 PciI A'CATG_T Cuts at: 4156 4156 Size: 5257 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5257 PmeI GTTT'AAAC Cuts at: 2561 2561 Size: 5257 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3326 3358 4839 4871 3326 Size: 32 1481 32 3712 Fragments arranged by size: 3712 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5257 PsiI TTA'TAA Cuts at: 2827 2827 Size: 5257 PstI C_TGCA'G Cuts at: 830 2609 830 Size: 1779 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5257 PvuII CAG'CTG Cuts at: 2083 2083 Size: 5257 SacI G_AGCT'C Cuts at: 2128 2128 Size: 5257 SalI G'TCGA_C Cuts at: 1936 2599 1936 Size: 663 4594 SbfI CC_TGCA'GG Cuts at: 2609 2609 Size: 5257 ScaI AGT'ACT Cuts at: 3505 5217 3505 Size: 1712 3545 SexAI A'CCwGG_T Cuts at: 2055 2055 Size: 5257 SfcI C'TryA_G Cuts at: 826 1045 2605 3740 4421 4612 826 Size: 219 1560 1135 681 191 1471 Fragments arranged by size: 1560 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2115 2276 2115 Size: 161 5096 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5257 SmaI CCC'GGG Cuts at: 1872 2584 1872 Size: 712 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3361 4262 4524 820 Size: 197 926 1418 901 262 1553 Fragments arranged by size: 1553 1418 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3048 493 Size: 2555 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5257 SphI G_CATG'C Cuts at: 1132 2615 1132 Size: 1483 3774 SspI AAT'ATT Cuts at: 5 52 3181 5 Size: 47 3129 2081 Fragments arranged by size: 3129 2081 47 StuI AGG'CCT Cuts at: 1517 2133 1517 Size: 616 4641 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2280 2696 513 Size: 552 939 115 161 416 3074 Fragments arranged by size: 3074 939 552 416 161 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3284 3301 3454 3639 4084 325 Size: 1255 1704 17 153 185 445 1498 Fragments arranged by size: 1704 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3503 5215 96 Size: 274 80 33 51 1443 1526 1712 138 Fragments arranged by size: 1712 1526 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5217 5249 5217 Size: 32 5225 Tth111I GACn'n_nGTC Cuts at: 1316 2537 1316 Size: 1221 4036 XbaI T'CTAG_A Cuts at: 2593 2593 Size: 5257 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2080 Size: 5257 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5257 XmaI C'CCGG_G Cuts at: 1870 2582 1870 Size: 712 4545 XmnI GAAnn'nnTTC Cuts at: 3386 3386 Size: 5257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3065 276 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI