Restriction map for
pFN21AB7545
(Linear) (Six-base) MAPSORT of: KIBB7545.seq Check: 8682 from: 1 to: 552
KIBB7545.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 330 552
Size: 330 222
AccI GT'mk_AC
Cuts at: 0 47 536 552
Size: 47 489 16
Fragments arranged by size:
489 47 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 552
Size: 449 103
AseI AT'TA_AT
Cuts at: 0 493 552
Size: 493 59
AvaI C'yCGr_G
Cuts at: 0 202 292 552
Size: 202 90 260
Fragments arranged by size:
260 202 90
BanII G_rGCy'C
Cuts at: 0 123 552
Size: 123 429
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 552
Size: 437 115
BbvCI CC'TCA_GC
Cuts at: 0 74 552
Size: 74 478
BglI GCCn_nnn'nGGC
Cuts at: 0 110 271 552
Size: 110 161 281
Fragments arranged by size:
281 161 110
Bme1580I G_kGCm'C
Cuts at: 0 392 552
Size: 392 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 552
Size: 107 445
Bpu10I CC'TnA_GC
Cuts at: 0 74 552
Size: 74 478
BsaAI yAC'GTr
Cuts at: 0 514 552
Size: 514 38
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 361 552
Size: 271 90 191
Fragments arranged by size:
271 191 90
BseYI C'CCAG_C
Cuts at: 0 78 552
Size: 78 474
BsiHKAI G_wGCw'C
Cuts at: 0 123 552
Size: 123 429
Bsp1286I G_dGCh'C
Cuts at: 0 123 392 552
Size: 123 269 160
Fragments arranged by size:
269 160 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 330 552
Size: 330 222
BsrFI r'CCGG_y
Cuts at: 0 261 552
Size: 261 291
BssSI C'ACGA_G
Cuts at: 0 166 552
Size: 166 386
BstZ17I GTA'TAC
Cuts at: 0 48 552
Size: 48 504
Bsu36I CC'TnA_GG
Cuts at: 0 149 552
Size: 149 403
BtgI C'CryG_G
Cuts at: 0 275 552
Size: 275 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 200 552
Size: 200 352
DrdI GACnn_nn'nnGTC
Cuts at: 0 532 552
Size: 532 20
EaeI y'GGCC_r
Cuts at: 0 111 272 552
Size: 111 161 280
Fragments arranged by size:
280 161 111
EcoICRI GAG'CTC
Cuts at: 0 121 552
Size: 121 431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 449 552
Size: 107 342 103
Fragments arranged by size:
342 107 103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 552
Size: 390 162
MscI TGG'CCA
Cuts at: 0 113 552
Size: 113 439
MslI CAynn'nnrTG
Cuts at: 0 544 552
Size: 544 8
MspA1I CmG'CkG
Cuts at: 0 78 552
Size: 78 474
NaeI GCC'GGC
Cuts at: 0 263 552
Size: 263 289
NcoI C'CATG_G
Cuts at: 0 275 552
Size: 275 277
NgoMIV G'CCGG_C
Cuts at: 0 261 552
Size: 261 291
NspI r_CATG'y
Cuts at: 0 303 552
Size: 303 249
PvuII CAG'CTG
Cuts at: 0 78 552
Size: 78 474
SacI G_AGCT'C
Cuts at: 0 123 552
Size: 123 429
SexAI A'CCwGG_T
Cuts at: 0 50 552
Size: 50 502
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 110 271 552
Size: 110 161 281
Fragments arranged by size:
281 161 110
StuI AGG'CCT
Cuts at: 0 128 552
Size: 128 424
StyI C'CwwG_G
Cuts at: 0 114 275 552
Size: 114 161 277
Fragments arranged by size:
277 161 114
Tth111I GACn'n_nGTC
Cuts at: 0 532 552
Size: 532 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 552
Size: 75 477
Enzymes that do cut:
AarI AccI AcuI AseI AvaI BanII BbsI BbvCI
BglI Bme1580I BpmI Bpu10I BsaAI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrFI BssSI BstZ17I Bsu36I BtgI BtgZI
DrdI EaeI EcoICRI Eco57MI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PvuII SacI SexAI SfiI
StuI StyI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BtsI ClaI
CspCI DraI DraIII EagI EarI EciI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI XbaI XhoI XmaI XmnI ZraI