Restriction map for
pFN21AB7539
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# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmFI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MaeII MflI MluI MroI
MseI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 370 GAATTC [GA]^AATT[CT]
ApaI 44 GGGCCC GGGCC^C
ApoI 370 GAATTC [GA]^AATT[CT]
AvaI 47 CCCGGG C^[CT]CG[GA]G
BanI 494 GGCACC G^G[CT][GA]CC
BcgI 280 CGAGAGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 196 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 393 CTGCAC GTGCAG(16/14)
BsmAI 144 GTCTC GTCTC(1/5)
BsmBI 143 CGTCTC CGTCTC(1/5)
BsmI 508 GAATGC GAATGC(1/-1)
BsoBI 47 CCCGGG C^[CT]CG[GA]G
BssSI 323 CTCGTG CACGAG(-5/-1)
BstAPI 8 GCAATGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 213 GGATG GGATG(2/0)
BstNI 310 CCAGG CC^[AT]GG
Cac8I 217 GCCTGC GC[ACGT]^[ACGT]GC
EarI 146 CTCTTC CTCTTC(1/4)
EcoRI 370 GAATTC G^AATTC
EcoRII 310 CCAGG ^CC[AT]GG
FokI 213 GGATG GGATG(9/13)
HapII 48 CCGG C^CGG
HincII 466 GTCAAC GT[CT]^[GA]AC
HindII 466 GTCAAC GT[CT]^[GA]AC
HpaII 48 CCGG C^CGG
Ksp632I 146 CTCTTC CTCTTC(1/4)
MamI 196 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 515 CAATTG C^AATTG
MspA1I 477 CCGCTG C[AC]G^C[GT]G
MspI 48 CCGG C^CGG
MunI 515 CAATTG C^AATTG
MvaI 310 CCAGG CC^[AT]GG
PstI 447 CTGCAG CTGCA^G
SfaNI 214 GATGC GCATC(5/9)
SmaI 47 CCCGGG CCC^GGG
Tsp45I 326 GTGAC ^GT[GC]AC
TspRI 40 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 47 CCCGGG C^CCGGG
XmaI 47 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 477 CCGC CCGC(-3/-1)
230 GCGG
BalI 36 TGGCCA TGG^CCA
240 TGGCCA
BanII 44 GGGCCC G[GA]GC[CT]^C
265 GAGCCC
BcnI 47 CCCGG CC^[GC]GG
48 CCGGG
BsiYI 58 CCTACTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
498 CCTTGCACGGG
BslI 58 CCTACTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
498 CCTTGCACGGG
Cfr13I 44 GGGCC G^G[ACGT]CC
45 GGCCC
DpnII 32 GATC ^GATC
202 GATC
DsaI 72 CCGTGG C^C[GA][CT]GG
93 CCGTGG
EaeI 36 TGGCCA [CT]^GGCC[GA]
240 TGGCCA
EcoT14I 191 CCAAGG C^C[AT][AT]GG
251 CCAAGG
HinfI 175 GAGTC G^A[ACGT]TC
487 GAGTC
HphI 172 GGTGA GGTGA(8/7)
421 GGTGA
MaeIII 326 GTGAC ^GT[ACGT]AC
473 GTTAC
MboI 32 GATC ^GATC
202 GATC
MboII 147 TCTTC GAAGA(8/7)
204 TCTTC
MluNI 36 TGGCCA TGG^CCA
240 TGGCCA
MscI 36 TGGCCA TGG^CCA
240 TGGCCA
NciI 47 CCCGG CC^[GC]GG
48 CCGGG
NdeII 32 GATC ^GATC
202 GATC
NlaIII 337 CATG CATG^
433 CATG
PleI 175 GAGTC GAGTC(4/5)
487 GAGTC
Sau3AI 32 GATC ^GATC
202 GATC
Sau96I 44 GGGCC G^G[ACGT]CC
45 GGCCC
StyI 191 CCAAGG C^C[AT][AT]GG
251 CCAAGG
Tsp509I 371 AATT ^AATT
516 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
BbvI BmyI BsaJI Bsp1286I BsrDI
BsrI BssKI Fnu4HI HaeIII ItaI
MnlI MslI MwoI NlaIV ScrFI
SfcI TseI