Restriction map for
pFN21AB7538
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 69 GAATTC [GA]^AATT[CT]
AlwI 658 GGATC GGATC(4/5)
AocI 541 CCTCAGG CC^T[ACGT]AGG
ApoI 69 GAATTC [GA]^AATT[CT]
Asp700I 336 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 140 GAGCTC G[AT]GC[AT]^C
AvaI 180 CTCGAG C^[CT]CG[GA]G
AvaII 322 GGTCC G^G[AT]CC
BalI 576 TGGCCA TGG^CCA
BbsI 15 GAAGAC GAAGAC(2/6)
BpuAI 15 GAAGAC GAAGAC(2/6)
BsiHKAI 140 GAGCTC G[AT]GC[AT]^C
BsmBI 248 CGTCTC CGTCTC(1/5)
BsoBI 180 CTCGAG C^[CT]CG[GA]G
BspDI 755 ATCGAT AT^CGAT
BsrBI 452 CCGCTC CCGCTC(-3/-3)
BssSI 653 CTCGTG CACGAG(-5/-1)
BstYI 658 GGATCT [GA]^GATC[CT]
Bsu36I 541 CCTCAGG CC^T[ACGT]AGG
Cac8I 409 GCGGGC GC[ACGT]^[ACGT]GC
ClaI 755 ATCGAT AT^CGAT
DpnII 659 GATC ^GATC
DraII 364 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 576 TGGCCA [CT]^GGCC[GA]
EarI 604 CTCTTC CTCTTC(1/4)
Eco81I 541 CCTCAGG CC^T[ACGT]AGG
EcoO109I 364 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 69 GAATTC G^AATTC
Ksp632I 604 CTCTTC CTCTTC(1/4)
MaeII 492 ACGT A^CGT
MboI 659 GATC ^GATC
MflI 658 GGATCT [GA]^GATC[CT]
MluNI 576 TGGCCA TGG^CCA
MscI 576 TGGCCA TGG^CCA
MslI 327 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 659 GATC ^GATC
PaeR7I 180 CTCGAG C^TCGAG
PleI 183 GAGTC GAGTC(4/5)
PstI 763 CTGCAG CTGCA^G
SacI 140 GAGCTC GAGCT^C
SapI 603 GCTCTTC GCTCTTC(1/4)
Sau3AI 659 GATC ^GATC
SexAI 461 ACCTGGT A^CC[AT]GGT
SfcI 763 CTGCAG C^T[GA][CT]AG
SmlI 180 CTCGAG C^T[CT][GA]AG
Tsp45I 283 GTCAC ^GT[GC]AC
XcmI 730 CCACCAAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 180 CTCGAG C^TCGAG
XhoII 658 GGATCT [GA]^GATC[CT]
XmnI 336 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 277 GTAC GT^AC
668 GTAC
BanI 412 GGCACC G^G[CT][GA]CC
458 GGCACC
BcnI 74 CCCGG CC^[GC]GG
619 CCCGG
BfaI 359 CTAG C^TAG
532 CTAG
BpmI 135 CTCCAG CTGGAG(16/14)
514 CTCCAG
BseRI 399 CTCCTC GAGGAG(10/8)
511 CTCCTC
BspMI 611 ACCTGC ACCTGC(4/8)
761 ACCTGC
BsrI 740 ACTGG ACTGG(1/-1)
665 CCAGT
DsaI 253 CCATGG C^C[GA][CT]GG
326 CCATGG
FauI 451 CCCGC CCCGC(4/6)
409 GCGGG
HphI 89 TCACC GGTGA(8/7)
346 TCACC
MaeI 359 CTAG C^TAG
532 CTAG
MaeIII 242 GTTAC ^GT[ACGT]AC
283 GTCAC
NciI 74 CCCGG CC^[GC]GG
619 CCCGG
NcoI 253 CCATGG C^CATGG
326 CCATGG
NspI 221 GCATGT [GA]CATG^[CT]
312 GCATGT
RsaI 277 GTAC GT^AC
668 GTAC
TaqI 181 TCGA T^CGA
756 TCGA
TfiI 336 GAATC G^A[AT]TC
547 GAATC
Tsp509I 70 AATT ^AATT
170 AATT
TspRI 235 CAGTG CA[GC]TG[ACGT][ACGT]^
739 CACTG
TthHB8I 181 TCGA T^CGA
756 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI BstXI
Cfr13I DdeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboII MnlI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI StyI TseI