(Circular) (Six-base) MAPSORT of: pFN21AB7536.seq Check: 2732 from: 1 to: 5476 pFN21AB7536 5476 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2711 3286 278 Size: 53 83 186 936 1175 575 2468 Fragments arranged by size: 2468 1175 936 575 186 83 53 AccI GT'mk_AC Cuts at: 1937 2819 1937 Size: 882 4594 Acc65I G'GTAC_C Cuts at: 2395 2797 2395 Size: 402 5074 AclI AA'CG_TT Cuts at: 1563 3603 3976 1563 Size: 2040 373 3063 Fragments arranged by size: 3063 2040 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3539 4923 5191 1632 Size: 1907 1384 268 1917 Fragments arranged by size: 1917 1907 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 4375 5352 1203 Size: 3172 977 1327 Fragments arranged by size: 3172 1327 977 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 4205 4205 Size: 5476 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2465 1184 Size: 1281 4195 AlwNI CAG_nnn'CTG Cuts at: 2410 3219 4791 2410 Size: 809 1572 3095 Fragments arranged by size: 3095 1572 809 ApaI G_GGCC'C Cuts at: 2294 2372 2294 Size: 78 5398 ApaLI G'TGCA_C Cuts at: 2594 3533 4689 2594 Size: 939 1156 3381 Fragments arranged by size: 3381 1156 939 ApoI r'AATT_y Cuts at: 1493 2785 3011 3163 5272 1493 Size: 1292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 152 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2528 2801 1870 Size: 73 585 273 4545 Fragments arranged by size: 4545 585 273 73 BamHI G'GATC_C Cuts at: 2806 3173 2806 Size: 367 5109 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2150 2168 2281 2324 Size: 325 217 627 351 12 18 113 43 Cuts at: 2324 2395 2490 2797 4252 618 Size: 71 95 307 1455 1842 Fragments arranged by size: 1842 1455 627 351 325 307 217 113 95 71 43 18 12 BanII G_rGCy'C Cuts at: 1391 2294 2372 2795 1391 Size: 903 78 423 4072 Fragments arranged by size: 4072 903 423 78 BbeI G_GCGC'C Cuts at: 2142 2154 2172 2285 2328 2142 Size: 12 18 113 43 5290 Fragments arranged by size: 5290 113 43 18 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2098 928 Size: 971 199 4306 Fragments arranged by size: 4306 971 199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3667 3701 1903 Size: 34 50 34 1646 34 3678 Fragments arranged by size: 3678 1646 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4087 136 Size: 107 122 71 631 605 2415 1525 Fragments arranged by size: 2415 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2893 2893 Size: 5476 Bme1580I G_kGCm'C Cuts at: 1790 2294 2372 2432 2570 2598 3537 4693 1790 Size: 504 78 60 138 28 939 1156 2573 Fragments arranged by size: 2573 1156 939 504 138 78 60 28 BmgBI CAC'GTC Cuts at: 1318 2563 1318 Size: 1245 4231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3207 4165 442 Size: 416 53 83 399 24 1790 958 1753 Fragments arranged by size: 1790 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4136 1239 Size: 2897 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3601 4466 4764 3601 Size: 865 298 4313 Fragments arranged by size: 4313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 4139 882 Size: 617 1041 1599 2219 Fragments arranged by size: 2219 1599 1041 617 BsaAI yAC'GTr Cuts at: 493 1204 2272 3267 493 Size: 711 1068 995 2702 Fragments arranged by size: 2702 1068 995 711 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 2151 2169 2282 Size: 53 83 186 936 606 12 18 113 Cuts at: 2282 2325 2351 2708 2723 3283 3665 4300 275 Size: 43 26 357 15 560 382 635 1451 Fragments arranged by size: 1451 936 635 606 560 382 357 186 113 83 53 43 26 18 15 12 BsaWI w'CCGG_w Cuts at: 2728 3909 4307 4581 4728 5139 2728 Size: 1181 398 274 147 411 3065 Fragments arranged by size: 3065 1181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 2609 2639 1178 Size: 30 165 30 1134 30 42 30 4015 Fragments arranged by size: 4015 1134 165 42 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 2621 2690 1186 Size: 960 475 69 3972 Fragments arranged by size: 3972 960 475 69 BseYI C'CCAG_C Cuts at: 2320 2328 4679 5175 2320 Size: 8 2351 496 2621 Fragments arranged by size: 2621 2351 496 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 2252 2294 2453 1163 Size: 573 366 150 42 159 4186 Fragments arranged by size: 4186 573 366 159 150 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2051 2217 2926 3687 4715 1540 Size: 275 186 50 166 709 761 1028 2301 Fragments arranged by size: 2301 1028 761 709 275 186 166 50 BsiHKAI G_wGCw'C Cuts at: 2339 2598 3537 3622 4693 2339 Size: 259 939 85 1071 3122 Fragments arranged by size: 3122 1071 939 259 85 BsmI GAATG_Cn' Cuts at: 2987 3080 2987 Size: 93 5383 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2294 2339 2372 2432 2570 2598 2795 Size: 399 504 45 33 60 138 28 197 Cuts at: 2795 3537 3622 4693 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 399 197 138 85 60 45 33 28 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2831 844 Size: 1987 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3363 1394 Size: 429 1540 3507 Fragments arranged by size: 3507 1540 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3971 4145 65 Size: 1183 2723 174 1396 Fragments arranged by size: 2723 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 4120 4307 1700 Size: 110 264 2046 187 2869 Fragments arranged by size: 2869 2046 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5476 BssSI C'ACGA_G Cuts at: 3536 4548 3536 Size: 1012 4464 BstAPI GCAn_nnn'nTGC Cuts at: 794 2407 794 Size: 1613 3863 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2806 3173 3558 3575 5027 5113 5125 5471 Size: 834 367 385 17 1452 86 12 346 Cuts at: 5471 1972 Size: 1977 Fragments arranged by size: 1977 1452 834 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2187 2217 2286 513 Size: 552 1122 30 69 3703 Fragments arranged by size: 3703 1122 552 69 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2339 2992 3786 3806 2339 Size: 653 794 20 4009 Fragments arranged by size: 4009 794 653 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2713 4483 809 Size: 507 1397 1770 1802 Fragments arranged by size: 1802 1770 1397 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 2214 2923 Size: 54 1425 50 275 18 218 166 709 Cuts at: 2923 3812 5441 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 709 275 218 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2048 2214 2923 1537 Size: 275 236 166 709 4090 Fragments arranged by size: 4090 709 275 236 166 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 2729 2736 3406 1353 Size: 1190 186 7 670 3423 Fragments arranged by size: 3423 1190 670 186 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2494 4063 4437 4583 387 Size: 1064 138 905 1569 374 146 1280 Fragments arranged by size: 1569 1280 1064 905 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3539 4136 4923 5191 1239 Size: 393 1907 597 787 268 1524 Fragments arranged by size: 1907 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2540 2603 2540 Size: 63 5413 EcoO109I rG'GnC_Cy Cuts at: 2368 2369 2368 Size: 1 5475 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 2142 2154 2172 2285 2328 4623 1678 Size: 464 12 18 113 43 2295 2531 Fragments arranged by size: 2531 2295 464 113 43 18 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2750 2782 4117 4149 4191 Size: 32 135 32 1178 32 1335 32 42 Cuts at: 4191 4223 1373 Size: 32 2626 Fragments arranged by size: 2626 1335 1178 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2820 3066 669 Size: 988 281 882 246 3079 Fragments arranged by size: 3079 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KasI G'GCGC_C Cuts at: 2138 2150 2168 2281 2324 2138 Size: 12 18 113 43 5290 Fragments arranged by size: 5290 113 43 18 12 KpnI G_GTAC'C Cuts at: 2399 2801 2399 Size: 402 5074 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2661 4590 4774 1305 Size: 1356 1929 184 2007 Fragments arranged by size: 2007 1929 1356 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3654 Fragments arranged by size: 3654 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 3434 3793 3952 518 Size: 648 1257 1011 359 159 2042 Fragments arranged by size: 2042 1257 1011 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2145 2219 2226 2266 2320 2467 2581 2892 Size: 539 74 7 40 54 147 114 311 Cuts at: 2892 3569 4717 4962 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 539 311 245 147 114 74 54 40 7 NarI GG'CG_CC Cuts at: 2139 2151 2169 2282 2325 2139 Size: 12 18 113 43 5290 Fragments arranged by size: 5290 113 43 18 12 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4924 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2020 2834 4379 1132 Size: 97 791 814 1545 2229 Fragments arranged by size: 2229 1545 814 791 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5476 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5476 PfoI T'CCnGG_A Cuts at: 2541 2541 Size: 5476 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PmlI CAC'GTG Cuts at: 2272 2272 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PspOMI G'GGCC_C Cuts at: 2290 2368 2290 Size: 78 5398 PstI C_TGCA'G Cuts at: 830 2454 2828 830 Size: 1624 374 3478 Fragments arranged by size: 3478 1624 374 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 PvuII CAG'CTG Cuts at: 2266 2467 2581 2266 Size: 201 114 5161 Fragments arranged by size: 5161 201 114 SacII CC_GC'GG Cuts at: 2220 2220 Size: 5476 SalI G'TCGA_C Cuts at: 1936 2818 1936 Size: 882 4594 SbfI CC_TGCA'GG Cuts at: 2828 2828 Size: 5476 ScaI AGT'ACT Cuts at: 3724 5436 3724 Size: 1712 3764 SfcI C'TryA_G Cuts at: 826 1045 2450 2824 3959 4640 4831 826 Size: 219 1405 374 1135 681 191 1471 Fragments arranged by size: 1471 1405 1135 681 374 219 191 SfoI GGC'GCC Cuts at: 2140 2152 2170 2283 2326 2140 Size: 12 18 113 43 5290 Fragments arranged by size: 5290 113 43 18 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2803 1872 Size: 931 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2528 3580 4481 4743 820 Size: 197 926 585 1052 901 262 1553 Fragments arranged by size: 1553 1052 926 901 585 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 3400 5 Size: 47 3348 2081 Fragments arranged by size: 3348 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5476 StyI C'CwwG_G Cuts at: 513 1065 2915 513 Size: 552 1850 3074 Fragments arranged by size: 3074 1850 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3503 3520 3673 3858 4303 325 Size: 1255 1923 17 153 185 445 1498 Fragments arranged by size: 1923 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3722 5434 96 Size: 274 80 33 51 1443 1745 1712 138 Fragments arranged by size: 1745 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5436 5468 5436 Size: 32 5444 Tth111I GACn'n_nGTC Cuts at: 1316 2710 1316 Size: 1394 4082 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 2528 1943 Size: 585 4891 XmaI C'CCGG_G Cuts at: 1870 2801 1870 Size: 931 4545 XmnI GAAnn'nnTTC Cuts at: 2537 3605 2537 Size: 1068 4408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2709 3284 276 Size: 53 83 186 936 1175 575 2468 Fragments arranged by size: 2468 1175 936 575 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI