Restriction map for
pFN21AB7524
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MslI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacII SalI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 229 GAATTT [GA]^AATT[CT]
ApoI 229 GAATTT [GA]^AATT[CT]
Asp700I 43 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 698 CTCGGG C^[CT]CG[GA]G
AviII 494 TGCGCA TGC^GCA
BalI 134 TGGCCA TGG^CCA
BamHI 573 GGATCC G^GATCC
BanII 168 GAGCTC G[GA]GC[CT]^C
BfaI 91 CTAG C^TAG
BsaI 609 GGTCTC GGTCTC(1/5)
BsgI 247 GTGCAG GTGCAG(16/14)
BsmAI 610 GTCTC GTCTC(1/5)
BsmFI 86 GGGAC GGGAC(10/14)
BsoBI 698 CTCGGG C^[CT]CG[GA]G
BspHI 80 TCATGA T^CATGA
CfoI 495 GCGC GCG^C
EaeI 134 TGGCCA [CT]^GGCC[GA]
EarI 369 CTCTTC CTCTTC(1/4)
FspI 494 TGCGCA TGC^GCA
HapII 396 CCGG C^CGG
HhaI 495 GCGC GCG^C
HinP1I 495 GCGC G^CGC
HpaII 396 CCGG C^CGG
Ksp632I 369 CTCTTC CTCTTC(1/4)
MaeI 91 CTAG C^TAG
MluNI 134 TGGCCA TGG^CCA
MscI 134 TGGCCA TGG^CCA
MseI 484 TTAA T^TAA
MspI 396 CCGG C^CGG
PflMI 653 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 80 TCATGA T^CATGA
SacI 168 GAGCTC GAGCT^C
SapI 368 GCTCTTC GCTCTTC(1/4)
SexAI 605 ACCAGGT A^CC[AT]GGT
SfaNI 516 GATGC GCATC(5/9)
SmlI 331 CTCAAG C^T[CT][GA]AG
Tru9I 484 TTAA T^TAA
Van91I 653 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 43 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspHI 168 GAGCTC G[AT]GC[AT]^C
425 GTGCTC
AvaII 87 GGACC G^G[AT]CC
108 GGACC
BsaJI 173 CCGAGG C^C[ACGT][ACGT]GG
697 CCTCGG
BsiHKAI 168 GAGCTC G[AT]GC[AT]^C
425 GTGCTC
BssSI 422 CTCGTG CACGAG(-5/-1)
490 CTCGTG
BstYI 200 AGATCC [GA]^GATC[CT]
573 GGATCC
Cac8I 157 GCCCGC GC[ACGT]^[ACGT]GC
252 GCTTGC
DraII 86 GGGACCT [GA]G^G[ACGT]CC[CT]
107 AGGACCT
EcoO109I 86 GGGACCT [GA]G^G[ACGT]CC[CT]
107 AGGACCT
FauI 158 CCCGC CCCGC(4/6)
7 GCGGG
HinfI 349 GAGTC G^A[ACGT]TC
399 GACTC
HphI 235 GGTGA GGTGA(8/7)
603 TCACC
MflI 200 AGATCC [GA]^GATC[CT]
573 GGATCC
NlaIII 81 CATG CATG^
258 CATG
PleI 399 GACTC GAGTC(4/5)
349 GAGTC
PpuMI 86 GGGACCT [GA]G^G[AT]CC[CT]
107 AGGACCT
XcmI 204 CCAGGAAGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
606 CCAGGTCTCCTATGG
XhoII 200 AGATCC [GA]^GATC[CT]
573 GGATCC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI Tsp45I Tsp509I TspRI