(Circular) (Six-base) MAPSORT of: pFN21AB7518.seq Check: 9276 from: 1 to: 5443 pFN21AB7518 5443 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3253 278 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2786 1937 Size: 849 4594 Acc65I G'GTAC_C Cuts at: 2764 2764 Size: 5443 AclI AA'CG_TT Cuts at: 1563 3570 3943 1563 Size: 2007 373 3063 Fragments arranged by size: 3063 2007 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2723 3506 4890 5158 1632 Size: 1091 783 1384 268 1917 Fragments arranged by size: 1917 1384 1091 783 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5246 AflIII A'CryG_T Cuts at: 1203 2135 2530 4342 5319 1203 Size: 932 395 1812 977 1327 Fragments arranged by size: 1812 1327 977 932 395 AgeI A'CCGG_T Cuts at: 4274 4274 Size: 5443 AhdI GACnn_n'nnGTC Cuts at: 4172 4172 Size: 5443 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5443 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2286 1184 Size: 1102 4341 AlwNI CAG_nnn'CTG Cuts at: 2654 3186 4758 2654 Size: 532 1572 3339 Fragments arranged by size: 3339 1572 532 ApaLI G'TGCA_C Cuts at: 3500 4656 3500 Size: 1156 4287 ApoI r'AATT_y Cuts at: 1493 2040 2564 2576 2752 2978 3130 5239 1493 Size: 547 524 12 176 226 152 2109 1697 Fragments arranged by size: 2109 1697 547 524 226 176 152 12 AseI AT'TA_AT Cuts at: 160 1032 3997 160 Size: 872 2965 1606 Fragments arranged by size: 2965 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 AvaI C'yCGr_G Cuts at: 1870 1943 2768 1870 Size: 73 825 4545 Fragments arranged by size: 4545 825 73 BamHI G'GATC_C Cuts at: 2064 2773 3140 2064 Size: 709 367 4367 Fragments arranged by size: 4367 709 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2764 4219 618 Size: 325 217 627 539 438 1455 1842 Fragments arranged by size: 1842 1455 627 539 438 325 217 BanII G_rGCy'C Cuts at: 1391 2232 2762 1391 Size: 841 530 4072 Fragments arranged by size: 4072 841 530 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4472 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3634 3668 1903 Size: 34 1697 34 3678 Fragments arranged by size: 3678 1697 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3335 4545 3335 Size: 1210 4233 BclI T'GATC_A Cuts at: 1548 1548 Size: 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4054 136 Size: 107 122 71 631 605 337 2045 1525 Fragments arranged by size: 2045 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5438 5438 Size: 5443 BlpI GC'TnA_GC Cuts at: 2860 2860 Size: 5443 Bme1580I G_kGCm'C Cuts at: 1790 3504 4660 1790 Size: 1714 1156 2573 Fragments arranged by size: 2573 1714 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3174 4132 442 Size: 416 53 83 399 24 1757 958 1753 Fragments arranged by size: 1757 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2331 2616 4103 1239 Size: 1092 285 1487 2579 Fragments arranged by size: 2579 1487 1092 285 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2208 3568 4433 4731 2208 Size: 1360 865 298 2920 Fragments arranged by size: 2920 1360 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2669 4106 882 Size: 617 1170 1437 2219 Fragments arranged by size: 2219 1437 1170 617 BsaAI yAC'GTr Cuts at: 493 1204 3234 493 Size: 711 2030 2702 Fragments arranged by size: 2702 2030 711 BsaBI GATnn'nnATC Cuts at: 5437 5437 Size: 5443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3250 3632 4267 275 Size: 53 83 186 936 1717 382 635 1451 Fragments arranged by size: 1717 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3876 4274 4548 4695 5106 3876 Size: 398 274 147 411 4213 Fragments arranged by size: 4213 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5218 Fragments arranged by size: 5218 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2681 1186 Size: 1495 3948 BseYI C'CCAG_C Cuts at: 2035 4646 5142 2035 Size: 2611 496 2336 Fragments arranged by size: 2611 2336 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2603 1163 Size: 573 867 4003 Fragments arranged by size: 4003 867 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2893 3654 4682 1540 Size: 275 186 892 761 1028 2301 Fragments arranged by size: 2301 1028 892 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3504 3589 4660 3504 Size: 85 1071 4287 Fragments arranged by size: 4287 1071 85 BsmI GAATG_Cn' Cuts at: 2954 3047 2954 Size: 93 5350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2762 3504 3589 4660 1391 Size: 399 442 530 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 530 442 399 85 BspEI T'CCGG_A Cuts at: 5106 5106 Size: 5443 BspHI T'CATG_A Cuts at: 2458 3332 5062 2458 Size: 874 1730 2839 Fragments arranged by size: 2839 1730 874 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2798 844 Size: 1954 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3330 1394 Size: 429 1507 3507 Fragments arranged by size: 3507 1507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2304 3938 4112 65 Size: 1183 1056 1634 174 1396 Fragments arranged by size: 1634 1396 1183 1056 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4087 4274 1700 Size: 110 2277 187 2869 Fragments arranged by size: 2869 2277 187 110 BsrGI T'GTAC_A Cuts at: 96 2689 96 Size: 2593 2850 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5443 BssSI C'ACGA_G Cuts at: 3503 4515 3503 Size: 1012 4431 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5443 BstBI TT'CG_AA Cuts at: 4247 4247 Size: 5443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5443 BstYI r'GATC_y Cuts at: 1972 2064 2773 3140 3525 3542 4994 5080 5092 Size: 92 709 367 385 17 1452 86 12 Cuts at: 5092 5438 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 709 385 367 346 92 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2664 2664 Size: 5443 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3952 Fragments arranged by size: 3952 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4704 Fragments arranged by size: 4704 683 56 BtsI GCAGTG_nn' Cuts at: 2376 2959 3753 3773 2376 Size: 583 794 20 4046 Fragments arranged by size: 4046 794 583 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5408 DraI TTT'AAA Cuts at: 2747 2747 Size: 5443 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4450 809 Size: 507 3134 1802 Fragments arranged by size: 3134 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2890 3779 5408 Size: 54 1425 50 275 18 1060 889 1629 Cuts at: 5408 8 Size: 43 Fragments arranged by size: 1629 1425 1060 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2890 1537 Size: 275 1078 4090 Fragments arranged by size: 4090 1078 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2457 3373 1353 Size: 1104 916 3423 Fragments arranged by size: 3423 1104 916 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4030 4404 4550 387 Size: 1064 138 2441 374 146 1280 Fragments arranged by size: 2441 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2616 2723 3506 4103 4890 5158 Size: 393 699 285 107 783 597 787 268 Cuts at: 5158 1239 Size: 1524 Fragments arranged by size: 1524 787 783 699 597 393 285 268 107 EcoNI CCTnn'n_nnAGG Cuts at: 2430 2430 Size: 5443 EcoO109I rG'GnC_Cy Cuts at: 2714 2714 Size: 5443 EcoRI G'AATT_C Cuts at: 2752 3130 2752 Size: 378 5065 EcoRV GAT'ATC Cuts at: 2020 2420 2020 Size: 400 5043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2136 2136 Size: 5443 FspI TGC'GCA Cuts at: 3949 3949 Size: 5443 HaeII r_GCGC'y Cuts at: 1678 4590 1678 Size: 2912 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 4084 4116 4158 Size: 32 135 32 752 32 1728 32 42 Cuts at: 4158 4190 1373 Size: 32 2626 Fragments arranged by size: 2626 1728 752 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2053 2787 3033 669 Size: 988 281 115 734 246 3079 Fragments arranged by size: 3079 988 734 281 246 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 5443 HpaI GTT'AAC Cuts at: 3033 3033 Size: 5443 KpnI G_GTAC'C Cuts at: 2768 2768 Size: 5443 MfeI C'AATT_G Cuts at: 3042 3042 Size: 5443 MluI A'CGCG_T Cuts at: 5319 5319 Size: 5443 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2176 4557 4741 1305 Size: 871 2381 184 2007 Fragments arranged by size: 2381 2007 871 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3621 Fragments arranged by size: 3621 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3401 3760 3919 518 Size: 648 2235 359 159 2042 Fragments arranged by size: 2235 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2260 2859 3536 4684 4929 1606 Size: 654 599 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 654 599 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3952 Fragments arranged by size: 3952 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5443 NotI GC'GGCC_GC Cuts at: 2890 2890 Size: 5443 NspI r_CATG'y Cuts at: 1132 1229 2139 2222 2801 4346 1132 Size: 97 910 83 579 1545 2229 Fragments arranged by size: 2229 1545 910 579 97 83 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5443 PciI A'CATG_T Cuts at: 2135 4342 2135 Size: 2207 3236 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5443 PfoI T'CCnGG_A Cuts at: 2091 2091 Size: 5443 PmeI GTTT'AAAC Cuts at: 2747 2747 Size: 5443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3512 3544 5025 5057 3512 Size: 32 1481 32 3898 Fragments arranged by size: 3898 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5443 PsiI TTA'TAA Cuts at: 2348 3013 2348 Size: 665 4778 PstI C_TGCA'G Cuts at: 830 2371 2795 830 Size: 1541 424 3478 Fragments arranged by size: 3478 1541 424 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5443 SalI G'TCGA_C Cuts at: 1936 2785 1936 Size: 849 4594 SapI GCTCTTCn'nnn_ Cuts at: 2457 2457 Size: 5443 SbfI CC_TGCA'GG Cuts at: 2795 2795 Size: 5443 ScaI AGT'ACT Cuts at: 3691 5403 3691 Size: 1712 3731 SfcI C'TryA_G Cuts at: 826 1045 2367 2791 3926 4607 4798 826 Size: 219 1322 424 1135 681 191 1471 Fragments arranged by size: 1471 1322 1135 681 424 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5443 SmaI CCC'GGG Cuts at: 1872 2770 1872 Size: 898 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2223 3547 4448 4710 820 Size: 197 926 280 1324 901 262 1553 Fragments arranged by size: 1553 1324 926 901 280 262 197 SnaBI TAC'GTA Cuts at: 493 3234 493 Size: 2741 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5443 SphI G_CATG'C Cuts at: 1132 2222 2801 1132 Size: 1090 579 3774 Fragments arranged by size: 3774 1090 579 SspI AAT'ATT Cuts at: 5 52 3367 5 Size: 47 3315 2081 Fragments arranged by size: 3315 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5443 StyI C'CwwG_G Cuts at: 513 1065 2004 2882 513 Size: 552 939 878 3074 Fragments arranged by size: 3074 939 878 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2587 3470 3487 3640 3825 4270 325 Size: 1255 1007 883 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1007 883 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2205 2689 3689 Size: 274 80 33 51 1443 228 484 1000 Cuts at: 3689 5401 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1000 484 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5403 5435 5403 Size: 32 5411 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5443 XbaI T'CTAG_A Cuts at: 2354 2779 2354 Size: 425 5018 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5443 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5443 XmaI C'CCGG_G Cuts at: 1870 2768 1870 Size: 898 4545 XmnI GAAnn'nnTTC Cuts at: 3572 3572 Size: 5443 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3251 276 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI