(Circular) (Six-base) MAPSORT of: pFN21AB7516.seq Check: 5745 from: 1 to: 5443 pFN21AB7516 5443 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3253 278 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2086 2786 1937 Size: 149 700 4594 Fragments arranged by size: 4594 700 149 Acc65I G'GTAC_C Cuts at: 2764 2764 Size: 5443 AclI AA'CG_TT Cuts at: 1563 2493 3570 3943 1563 Size: 930 1077 373 3063 Fragments arranged by size: 3063 1077 930 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 2570 3506 4890 5158 1632 Size: 454 484 936 1384 268 1917 Fragments arranged by size: 1917 1384 936 484 454 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5246 AflIII A'CryG_T Cuts at: 1203 4342 5319 1203 Size: 3139 977 1327 Fragments arranged by size: 3139 1327 977 AgeI A'CCGG_T Cuts at: 4274 4274 Size: 5443 AhdI GACnn_n'nnGTC Cuts at: 4172 4172 Size: 5443 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5443 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2083 1184 Size: 899 4544 AlwNI CAG_nnn'CTG Cuts at: 3186 4758 3186 Size: 1572 3871 ApaLI G'TGCA_C Cuts at: 2068 3500 4656 2068 Size: 1432 1156 2855 Fragments arranged by size: 2855 1432 1156 ApoI r'AATT_y Cuts at: 1493 2752 2978 3130 5239 1493 Size: 1259 226 152 2109 1697 Fragments arranged by size: 2109 1697 1259 226 152 AseI AT'TA_AT Cuts at: 160 1032 3997 160 Size: 872 2965 1606 Fragments arranged by size: 2965 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 AvaI C'yCGr_G Cuts at: 1870 1943 2768 1870 Size: 73 825 4545 Fragments arranged by size: 4545 825 73 BamHI G'GATC_C Cuts at: 2773 3140 2773 Size: 367 5076 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2764 4219 618 Size: 325 217 627 977 1455 1842 Fragments arranged by size: 1842 1455 977 627 325 217 BanII G_rGCy'C Cuts at: 1391 2762 1391 Size: 1371 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2558 928 Size: 971 659 3813 Fragments arranged by size: 3813 971 659 BbvCI CC'TCA_GC Cuts at: 2733 2733 Size: 5443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3634 3668 1903 Size: 34 1697 34 3678 Fragments arranged by size: 3678 1697 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3335 4545 3335 Size: 1210 4233 BclI T'GATC_A Cuts at: 1548 1548 Size: 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4054 136 Size: 107 122 71 631 605 2382 1525 Fragments arranged by size: 2382 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5438 5438 Size: 5443 BlpI GC'TnA_GC Cuts at: 2860 2860 Size: 5443 Bme1580I G_kGCm'C Cuts at: 1790 2044 2072 3504 4660 1790 Size: 254 28 1432 1156 2573 Fragments arranged by size: 2573 1432 1156 254 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3174 4132 442 Size: 416 53 83 399 24 1757 958 1753 Fragments arranged by size: 1757 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5443 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2076 4103 1239 Size: 837 2027 2579 Fragments arranged by size: 2579 2027 837 Bpu10I CC'TnA_GC Cuts at: 2523 2733 2523 Size: 210 5233 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3568 4433 4731 3568 Size: 865 298 4280 Fragments arranged by size: 4280 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4106 882 Size: 617 2607 2219 Fragments arranged by size: 2607 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3234 493 Size: 711 2030 2702 Fragments arranged by size: 2702 2030 711 BsaBI GATnn'nnATC Cuts at: 5437 5437 Size: 5443 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3250 3632 4267 275 Size: 53 83 186 936 1717 382 635 1451 Fragments arranged by size: 1717 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3876 4274 4548 4695 5106 3876 Size: 398 274 147 411 4213 Fragments arranged by size: 4213 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5218 Fragments arranged by size: 5218 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2750 1186 Size: 1564 3879 BseYI C'CCAG_C Cuts at: 2259 4646 5142 2259 Size: 2387 496 2560 Fragments arranged by size: 2560 2387 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4870 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2893 3654 4682 1540 Size: 275 186 892 761 1028 2301 Fragments arranged by size: 2301 1028 892 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2072 3504 3589 4660 2072 Size: 1432 85 1071 2855 Fragments arranged by size: 2855 1432 1071 85 BsmI GAATG_Cn' Cuts at: 2954 3047 2954 Size: 93 5350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2072 2762 3504 3589 4660 1391 Size: 399 254 28 690 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 690 399 254 85 28 BspEI T'CCGG_A Cuts at: 5106 5106 Size: 5443 BspHI T'CATG_A Cuts at: 3332 5062 3332 Size: 1730 3713 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2317 2798 844 Size: 1473 481 3489 Fragments arranged by size: 3489 1473 481 BsrBI CCG'CTC Cuts at: 1394 1823 3330 1394 Size: 429 1507 3507 Fragments arranged by size: 3507 1507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3938 4112 65 Size: 1183 2690 174 1396 Fragments arranged by size: 2690 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 4087 4274 1700 Size: 110 262 2015 187 2869 Fragments arranged by size: 2869 2015 262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5443 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5443 BssSI C'ACGA_G Cuts at: 3503 4515 3503 Size: 1012 4431 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5443 BstBI TT'CG_AA Cuts at: 4247 4247 Size: 5443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5443 BstYI r'GATC_y Cuts at: 1972 2689 2773 3140 3525 3542 4994 5080 5092 Size: 717 84 367 385 17 1452 86 12 Cuts at: 5092 5438 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 717 385 367 346 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3952 Fragments arranged by size: 3952 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4704 Fragments arranged by size: 4704 683 56 BtsI GCAGTG_nn' Cuts at: 2453 2959 3753 3773 2453 Size: 506 794 20 4123 Fragments arranged by size: 4123 794 506 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5408 DraI TTT'AAA Cuts at: 2209 2356 2418 2747 2209 Size: 147 62 329 4905 Fragments arranged by size: 4905 329 147 62 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4450 809 Size: 507 3134 1802 Fragments arranged by size: 3134 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2890 3779 5408 Size: 54 1425 50 275 18 1060 889 1629 Cuts at: 5408 8 Size: 43 Fragments arranged by size: 1629 1425 1060 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2890 1537 Size: 275 1078 4090 Fragments arranged by size: 4090 1078 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 3373 1353 Size: 981 1039 3423 Fragments arranged by size: 3423 1039 981 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2333 4030 4404 4550 387 Size: 1064 138 744 1697 374 146 1280 Fragments arranged by size: 1697 1280 1064 744 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2086 2570 3506 4103 4890 5158 Size: 393 444 10 484 936 597 787 268 Cuts at: 5158 1239 Size: 1524 Fragments arranged by size: 1524 936 787 597 484 444 393 268 10 EcoRI G'AATT_C Cuts at: 2752 3130 2752 Size: 378 5065 FspI TGC'GCA Cuts at: 3949 3949 Size: 5443 HaeII r_GCGC'y Cuts at: 1678 4590 1678 Size: 2912 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373 Size: 32 135 32 2512 32 42 32 2626 Fragments arranged by size: 2626 2512 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2087 2451 2787 3033 669 Size: 988 281 149 364 336 246 3079 Fragments arranged by size: 3079 988 364 336 281 246 149 HindIII A'AGCT_T Cuts at: 748 748 Size: 5443 HpaI GTT'AAC Cuts at: 2451 3033 2451 Size: 582 4861 KpnI G_GTAC'C Cuts at: 2768 2768 Size: 5443 MfeI C'AATT_G Cuts at: 3042 3042 Size: 5443 MluI A'CGCG_T Cuts at: 5319 5319 Size: 5443 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2246 2659 4557 4741 1305 Size: 941 413 1898 184 2007 Fragments arranged by size: 2007 1898 941 413 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3621 Fragments arranged by size: 3621 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3401 3760 3919 518 Size: 648 2235 359 159 2042 Fragments arranged by size: 2235 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2859 3536 4684 4929 1606 Size: 1253 677 1148 245 2120 Fragments arranged by size: 2120 1253 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3952 Fragments arranged by size: 3952 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5443 NotI GC'GGCC_GC Cuts at: 2890 2890 Size: 5443 NspI r_CATG'y Cuts at: 1132 1229 2026 2615 2801 4346 1132 Size: 97 797 589 186 1545 2229 Fragments arranged by size: 2229 1545 797 589 186 97 PasI CC'CwG_GG Cuts at: 1348 2044 1348 Size: 696 4747 PciI A'CATG_T Cuts at: 4342 4342 Size: 5443 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5443 PmeI GTTT'AAAC Cuts at: 2747 2747 Size: 5443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3512 3544 5025 5057 3512 Size: 32 1481 32 3898 Fragments arranged by size: 3898 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5443 PsiI TTA'TAA Cuts at: 2490 3013 2490 Size: 523 4920 PstI C_TGCA'G Cuts at: 830 2795 830 Size: 1965 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5443 SalI G'TCGA_C Cuts at: 1936 2085 2785 1936 Size: 149 700 4594 Fragments arranged by size: 4594 700 149 SbfI CC_TGCA'GG Cuts at: 2795 2795 Size: 5443 ScaI AGT'ACT Cuts at: 3691 5403 3691 Size: 1712 3731 SfcI C'TryA_G Cuts at: 826 1045 2235 2791 3926 4607 4798 826 Size: 219 1190 556 1135 681 191 1471 Fragments arranged by size: 1471 1190 1135 681 556 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5443 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5443 SmaI CCC'GGG Cuts at: 1872 2770 1872 Size: 898 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3547 4448 4710 820 Size: 197 926 1604 901 262 1553 Fragments arranged by size: 1604 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3234 493 Size: 2741 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5443 SphI G_CATG'C Cuts at: 1132 2615 2801 1132 Size: 1483 186 3774 Fragments arranged by size: 3774 1483 186 SspI AAT'ATT Cuts at: 5 52 3367 5 Size: 47 3315 2081 Fragments arranged by size: 3315 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5443 StyI C'CwwG_G Cuts at: 513 1065 2004 2882 513 Size: 552 939 878 3074 Fragments arranged by size: 3074 939 878 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3470 3487 3640 3825 4270 325 Size: 1255 1890 17 153 185 445 1498 Fragments arranged by size: 1890 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3689 5401 96 Size: 274 80 33 51 1443 1712 1712 138 Fragments arranged by size: 1712 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5403 5435 5403 Size: 32 5411 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5443 XbaI T'CTAG_A Cuts at: 2779 2779 Size: 5443 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5443 XmaI C'CCGG_G Cuts at: 1870 2768 1870 Size: 898 4545 XmnI GAAnn'nnTTC Cuts at: 3572 3572 Size: 5443 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3251 276 Size: 53 83 186 936 1717 2468 Fragments arranged by size: 2468 1717 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI