Restriction map for
pFN21AB7514
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvrII BalI
BamHI BanI BbeI BcgI BciVI
BclI BfrI BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FauI FbaI FseI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 257 GTAC GT^AC
AocI 185 CCTCAGG CC^T[ACGT]AGG
Asp700I 294 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 371 CTCGGG C^[CT]CG[GA]G
AvaII 216 GGACC G^G[AT]CC
AviII 524 TGCGCA TGC^GCA
BbrPI 504 CACGTG CAC^GTG
BbsI 647 GTCTTC GAAGAC(2/6)
BbvCI 7 GCTGAGG CCTCAGC(-5/-2)
BcnI 169 CCCGG CC^[GC]GG
BfaI 199 CTAG C^TAG
BglI 463 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 78 GCTGAGC GC^T[ACGT]AGC
BpmI 289 CTGGAG CTGGAG(16/14)
Bpu1102I 78 GCTGAGC GC^T[ACGT]AGC
BpuAI 647 GTCTTC GAAGAC(2/6)
BsaAI 504 CACGTG [CT]AC^GT[GA]
BsgI 376 GTGCAG GTGCAG(16/14)
BsmFI 640 GTCCC GGGAC(10/14)
BsoBI 371 CTCGGG C^[CT]CG[GA]G
BsrI 464 CCAGT ACTGG(1/-1)
BssSI 543 CACGAG CACGAG(-5/-1)
BstXI 479 CCACAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 185 CCTCAGG CC^T[ACGT]AGG
CelII 78 GCTGAGC GC^T[ACGT]AGC
DsaI 622 CCATGG C^C[GA][CT]GG
Eco81I 185 CCTCAGG CC^T[ACGT]AGG
EcoNI 637 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 622 CCATGG C^C[AT][AT]GG
EcoT22I 681 ATGCAT ATGCA^T
FspI 524 TGCGCA TGC^GCA
HapII 170 CCGG C^CGG
HinfI 393 GAATC G^A[ACGT]TC
HpaII 170 CCGG C^CGG
MaeI 199 CTAG C^TAG
MslI 669 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 170 CCGG C^CGG
NciI 169 CCCGG CC^[GC]GG
NcoI 622 CCATGG C^CATGG
NsiI 681 ATGCAT ATGCA^T
PmaCI 504 CACGTG CAC^GTG
PmlI 504 CACGTG CAC^GTG
PstI 27 CTGCAG CTGCA^G
RsaI 257 GTAC GT^AC
SapI 237 GAAGAGC GCTCTTC(1/4)
SfcI 27 CTGCAG C^T[GA][CT]AG
SmlI 563 CTCAAG C^T[CT][GA]AG
StuI 115 AGGCCT AGG^CCT
StyI 622 CCATGG C^C[AT][AT]GG
TfiI 393 GAATC G^A[AT]TC
XmnI 294 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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BglII 296 AGATCT A^GATCT
380 AGATCT
BstYI 296 AGATCT [GA]^GATC[CT]
380 AGATCT
CfoI 525 GCGC GCG^C
616 GCGC
EarI 160 GAAGAG CTCTTC(1/4)
237 GAAGAG
HhaI 525 GCGC GCG^C
616 GCGC
HinP1I 525 GCGC G^CGC
616 GCGC
Ksp632I 160 GAAGAG CTCTTC(1/4)
237 GAAGAG
MaeII 323 ACGT A^CGT
505 ACGT
MflI 296 AGATCT [GA]^GATC[CT]
380 AGATCT
NlaIII 283 CATG CATG^
623 CATG
XcmI 613 CCAGCGCACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
697 CCACACCCCAAATGG
XhoII 296 AGATCT [GA]^GATC[CT]
380 AGATCT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
BanII BbvI BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HphI ItaI MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI TseI TspRI