(Circular) (Six-base) MAPSORT of: pFN21AB7514.seq Check: 149 from: 1 to: 5440 pFN21AB7514 5440 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 1937 Size: 846 4594 Acc65I G'GTAC_C Cuts at: 2761 2761 Size: 5440 AclI AA'CG_TT Cuts at: 1563 3567 3940 1563 Size: 2004 373 3063 Fragments arranged by size: 3063 2004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3503 4887 5155 1632 Size: 1871 1384 268 1917 Fragments arranged by size: 1917 1871 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 4339 5316 1203 Size: 3136 977 1327 Fragments arranged by size: 3136 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2696 1184 Size: 1512 3928 AlwNI CAG_nnn'CTG Cuts at: 2198 2458 2724 3183 4755 2198 Size: 260 266 459 1572 2883 Fragments arranged by size: 2883 1572 459 266 260 ApaI G_GGCC'C Cuts at: 2024 2174 2508 2024 Size: 150 334 4956 Fragments arranged by size: 4956 334 150 ApaLI G'TGCA_C Cuts at: 3497 4653 3497 Size: 1156 4284 ApoI r'AATT_y Cuts at: 1493 2749 2975 3127 5236 1493 Size: 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2376 2765 1870 Size: 73 433 389 4545 Fragments arranged by size: 4545 433 389 73 BamHI G'GATC_C Cuts at: 2770 3137 2770 Size: 367 5073 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2761 4216 618 Size: 325 217 627 974 1455 1842 Fragments arranged by size: 1842 1455 974 627 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2174 2449 2508 2759 1391 Size: 633 150 275 59 251 4072 Fragments arranged by size: 4072 633 275 251 150 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2645 928 Size: 971 746 3723 Fragments arranged by size: 3723 971 746 BbvCI CC'TCA_GC Cuts at: 2013 2013 Size: 5440 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3332 4542 3332 Size: 1210 4230 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BfrBI ATG'CAT Cuts at: 2688 2688 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2474 4051 136 Size: 107 122 71 631 605 802 1577 1525 Fragments arranged by size: 1577 1525 802 631 605 122 107 71 BglII A'GATC_T Cuts at: 2301 2385 5435 2301 Size: 84 3050 2306 Fragments arranged by size: 3050 2306 84 BlpI GC'TnA_GC Cuts at: 2084 2857 2084 Size: 773 4667 Bme1580I G_kGCm'C Cuts at: 1790 2024 2174 2508 3501 4657 1790 Size: 234 150 334 993 1156 2573 Fragments arranged by size: 2573 1156 993 334 234 150 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 4100 1239 Size: 1076 1785 2579 Fragments arranged by size: 2579 1785 1076 Bpu10I CC'TnA_GC Cuts at: 2013 2013 Size: 5440 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2553 3565 4430 4728 2553 Size: 1012 865 298 3265 Fragments arranged by size: 3265 1012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4103 882 Size: 617 2604 2219 Fragments arranged by size: 2604 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2511 3231 493 Size: 711 1307 720 2702 Fragments arranged by size: 2702 1307 720 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3247 3629 4264 275 Size: 53 83 186 936 1714 382 635 1451 Fragments arranged by size: 1714 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3873 4271 4545 4692 5103 3873 Size: 398 274 147 411 4210 Fragments arranged by size: 4210 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2245 2275 1178 Size: 30 165 30 842 30 4343 Fragments arranged by size: 4343 842 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 2532 2603 1186 Size: 960 386 71 4023 Fragments arranged by size: 4023 960 386 71 BseYI C'CCAG_C Cuts at: 2501 2617 4643 5139 2501 Size: 116 2026 496 2802 Fragments arranged by size: 2802 2026 496 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 1163 Size: 573 666 4201 Fragments arranged by size: 4201 666 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2890 3651 4679 1540 Size: 275 186 889 761 1028 2301 Fragments arranged by size: 2301 1028 889 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3501 3586 4657 3501 Size: 85 1071 4284 Fragments arranged by size: 4284 1071 85 BsmI GAATG_Cn' Cuts at: 2951 3044 2951 Size: 93 5347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2174 2449 2508 2759 3501 3586 Size: 399 234 150 275 59 251 742 85 Cuts at: 3586 4657 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 275 251 234 150 85 59 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2795 844 Size: 1951 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3327 1394 Size: 429 1504 3507 Fragments arranged by size: 3507 1504 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3935 4109 65 Size: 1183 2687 174 1396 Fragments arranged by size: 2687 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4084 4271 1700 Size: 110 2274 187 2869 Fragments arranged by size: 2869 2274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 2548 3500 4512 2548 Size: 952 1012 3476 Fragments arranged by size: 3476 1012 952 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5440 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstXI CCAn_nnnn'nTGG Cuts at: 2491 2491 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2301 2385 2770 3137 3522 3539 4991 5077 Size: 329 84 385 367 385 17 1452 86 Cuts at: 5077 5089 5435 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 385 367 346 329 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2191 2191 Size: 5440 BtgI C'CryG_G Cuts at: 513 1065 2627 513 Size: 552 1562 3326 Fragments arranged by size: 3326 1562 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2520 2956 3750 3770 2520 Size: 436 794 20 4190 Fragments arranged by size: 4190 794 436 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2887 3776 5405 Size: 54 1425 50 275 18 1057 889 1629 Cuts at: 5405 8 Size: 43 Fragments arranged by size: 1629 1425 1057 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2887 1537 Size: 275 1075 4090 Fragments arranged by size: 4090 1075 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 2237 3370 1353 Size: 807 77 1133 3423 Fragments arranged by size: 3423 1133 807 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2431 4027 4401 4547 387 Size: 1064 138 842 1596 374 146 1280 Fragments arranged by size: 1596 1280 1064 842 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 3503 4100 4887 5155 1239 Size: 393 683 1188 597 787 268 1524 Fragments arranged by size: 1524 1188 787 683 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2646 2646 Size: 5440 EcoO109I rG'GnC_Cy Cuts at: 2021 2071 2170 2171 2021 Size: 50 99 1 5290 Fragments arranged by size: 5290 99 50 1 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FspI TGC'GCA Cuts at: 2531 3946 2531 Size: 1415 4025 HaeII r_GCGC'y Cuts at: 1678 4587 1678 Size: 2909 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 4081 4113 4155 Size: 32 135 32 970 32 1507 32 42 Cuts at: 4155 4187 1373 Size: 32 2626 Fragments arranged by size: 2626 1507 970 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2784 3030 669 Size: 988 281 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2765 2765 Size: 5440 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4554 4738 1305 Size: 3249 184 2007 Fragments arranged by size: 3249 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3618 Fragments arranged by size: 3618 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2678 3398 3757 3916 518 Size: 648 1512 720 359 159 2042 Fragments arranged by size: 2042 1512 720 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2284 2347 2458 2578 2856 3533 4681 4926 Size: 678 63 111 120 278 677 1148 245 Cuts at: 4926 1606 Size: 2120 Fragments arranged by size: 2120 1148 678 677 278 245 120 111 63 NcoI C'CATG_G Cuts at: 513 1065 2627 513 Size: 552 1562 3326 Fragments arranged by size: 3326 1562 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2887 2887 Size: 5440 NsiI A_TGCA'T Cuts at: 2690 2690 Size: 5440 NspI r_CATG'y Cuts at: 1132 1229 2798 4343 1132 Size: 97 1569 1545 2229 Fragments arranged by size: 2229 1569 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5440 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PmlI CAC'GTG Cuts at: 2511 2511 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PspOMI G'GGCC_C Cuts at: 2020 2170 2504 2020 Size: 150 334 4956 Fragments arranged by size: 4956 334 150 PstI C_TGCA'G Cuts at: 830 2036 2792 830 Size: 1206 756 3478 Fragments arranged by size: 3478 1206 756 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 PvuII CAG'CTG Cuts at: 2284 2347 2458 2578 2284 Size: 63 111 120 5146 Fragments arranged by size: 5146 120 111 63 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SapI GCTCTTCn'nnn_ Cuts at: 2237 2237 Size: 5440 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SfcI C'TryA_G Cuts at: 826 1045 2032 2788 3923 4604 4795 826 Size: 219 987 756 1135 681 191 1471 Fragments arranged by size: 1471 1135 987 756 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2767 1872 Size: 895 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2568 3544 4445 4707 820 Size: 197 926 625 976 901 262 1553 Fragments arranged by size: 1553 976 926 901 625 262 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 2122 1517 Size: 605 4835 StyI C'CwwG_G Cuts at: 513 1065 2627 2879 513 Size: 552 1562 252 3074 Fragments arranged by size: 3074 1562 552 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2640 3467 3484 3637 3822 4267 325 Size: 1255 1060 827 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1060 827 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3686 5398 96 Size: 274 80 33 51 1443 1709 1712 138 Fragments arranged by size: 1712 1709 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2628 2660 5400 5432 2628 Size: 32 2740 32 2636 Fragments arranged by size: 2740 2636 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2625 2709 2625 Size: 84 5356 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2765 1870 Size: 895 4545 XmnI GAAnn'nnTTC Cuts at: 2303 3569 2303 Size: 1266 4174 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI