Restriction map for
pFN21AB7512
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MfeI MluI
MluNI MroI MscI MseI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AlwI 121 GATCC GGATC(4/5)
AspHI 91 GAGCAC G[AT]GC[AT]^C
AspI 258 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 278 CTCGAG C^[CT]CG[GA]G
AvaII 443 GGACC G^G[AT]CC
BanI 668 GGCACC G^G[CT][GA]CC
BbvCI 477 CCTCAGC CCTCAGC(-5/-2)
BcnI 693 CCCGG CC^[GC]GG
BmyI 91 GAGCAC G[AGT]GC[ACT]^C
BsaBI 115 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 91 GAGCAC G[AT]GC[AT]^C
BsmAI 276 GTCTC GTCTC(1/5)
BsmFI 222 GTCCC GGGAC(10/14)
BsoBI 278 CTCGAG C^[CT]CG[GA]G
Bsp1286I 91 GAGCAC G[AGT]GC[ACT]^C
BspDI 112 ATCGAT AT^CGAT
BspHI 191 TCATGA T^CATGA
BspMI 37 ACCTGC ACCTGC(4/8)
BsrI 526 CCAGT ACTGG(1/-1)
BstXI 306 CCACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 120 AGATCC [GA]^GATC[CT]
ClaI 112 ATCGAT AT^CGAT
DpnII 121 GATC ^GATC
DraII 659 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 124 CCACGG C^C[GA][CT]GG
EaeI 77 TGGCCG [CT]^GGCC[GA]
EcoO109I 659 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 206 CCCGC CCCGC(4/6)
HapII 694 CCGG C^CGG
HpaII 694 CCGG C^CGG
HphI 347 TCACC GGTGA(8/7)
MamI 115 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 121 GATC ^GATC
MflI 120 AGATCC [GA]^GATC[CT]
MspI 694 CCGG C^CGG
NciI 693 CCCGG CC^[GC]GG
NdeII 121 GATC ^GATC
NlaIV 668 GGCACC GG[ACGT]^[ACGT]CC
PaeR7I 278 CTCGAG C^TCGAG
PflFI 258 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 526 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 191 TCATGA T^CATGA
Sau3AI 121 GATC ^GATC
SmlI 278 CTCGAG C^T[CT][GA]AG
Tth111I 258 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 526 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 278 CTCGAG C^TCGAG
XhoII 120 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 7 enzymes) #
###################################
Cfr13I 443 GGACC G^G[ACGT]CC
660 GGGCC
HaeIII 78 GGCC GG^CC
661 GGCC
MwoI 311 GCAGTGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
482 GCTCCATGTGC
Sau96I 443 GGACC G^G[ACGT]CC
660 GGGCC
Tsp45I 334 GTGAC ^GT[GC]AC
590 GTCAC
Tsp509I 146 AATT ^AATT
682 AATT
TspRI 272 CAGTG CA[GC]TG[ACGT][ACGT]^
312 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI AlwNI BbvI BsaJI
BseRI BsiYI BslI BssKI BstF5I
BstNI Cac8I DdeI EarI EcoRII
Fnu4HI FokI HinfI ItaI Ksp632I
MaeIII MboII MnlI MslI MvaI
NlaIII PleI ScrFI SfaNI TaqI
TfiI TseI TthHB8I