(Circular) (Six-base) MAPSORT of: pFN21AB7451.seq Check: 413 from: 1 to: 5953 pFN21AB7451 5953 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2457 2949 3296 1937 Size: 520 492 347 4594 Fragments arranged by size: 4594 520 492 347 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4016 5400 5668 1632 Size: 2384 1384 268 1917 Fragments arranged by size: 2384 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3053 820 Size: 197 2036 3720 Fragments arranged by size: 3720 2036 197 AflIII A'CryG_T Cuts at: 1203 4852 5829 1203 Size: 3649 977 1327 Fragments arranged by size: 3649 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2840 1184 Size: 1656 4297 AlwNI CAG_nnn'CTG Cuts at: 3696 5268 3696 Size: 1572 4381 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 2983 3262 3488 3640 5749 1493 Size: 1490 279 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 279 226 152 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 2545 3278 1870 Size: 73 602 733 4545 Fragments arranged by size: 4545 733 602 73 BamHI G'GATC_C Cuts at: 2608 3130 3283 3650 2608 Size: 522 153 367 4911 Fragments arranged by size: 4911 522 367 153 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2210 3274 4729 618 Size: 325 217 627 227 196 1064 1455 1842 Fragments arranged by size: 1842 1455 1064 627 325 227 217 196 BanII G_rGCy'C Cuts at: 1391 2026 2250 2799 3272 1391 Size: 635 224 549 473 4072 Fragments arranged by size: 4072 635 549 473 224 BbeI G_GCGC'C Cuts at: 2018 2018 Size: 5953 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 928 Size: 971 252 4730 Fragments arranged by size: 4730 971 252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2279 2313 4144 4178 1903 Size: 34 50 34 258 34 1831 34 3678 Fragments arranged by size: 3678 1831 258 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 1548 Size: 5953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4564 136 Size: 107 122 71 631 605 2892 1525 Fragments arranged by size: 2892 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5948 5948 Size: 5953 BlpI GC'TnA_GC Cuts at: 2249 3370 2249 Size: 1121 4832 Bme1580I G_kGCm'C Cuts at: 1790 2829 4014 5170 1790 Size: 1039 1185 1156 2573 Fragments arranged by size: 2573 1185 1156 1039 BmgBI CAC'GTC Cuts at: 1318 2605 1318 Size: 1287 4666 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2816 3684 4642 Size: 416 53 83 399 24 1399 868 958 Cuts at: 4642 442 Size: 1753 Fragments arranged by size: 1753 1399 958 868 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2348 2348 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 2777 4613 1239 Size: 1025 513 1836 2579 Fragments arranged by size: 2579 1836 1025 513 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2128 2371 4078 4943 5241 2128 Size: 243 1707 865 298 2840 Fragments arranged by size: 2840 1707 865 298 243 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4616 882 Size: 617 3117 2219 Fragments arranged by size: 3117 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3744 493 Size: 711 2540 2702 Fragments arranged by size: 2702 2540 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 3121 3760 4142 Size: 53 83 186 936 482 1106 639 382 Cuts at: 4142 4777 275 Size: 635 1451 Fragments arranged by size: 1451 1106 936 639 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5728 Fragments arranged by size: 5728 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2140 2723 2726 2729 2732 1186 Size: 954 583 3 3 3 4407 Fragments arranged by size: 4407 954 583 3 3 3 BseYI C'CCAG_C Cuts at: 2286 2656 5156 5652 2286 Size: 370 2500 496 2587 Fragments arranged by size: 2587 2500 496 370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 2314 3113 1163 Size: 573 329 249 799 4003 Fragments arranged by size: 4003 799 573 329 249 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2205 3403 4164 5192 1540 Size: 275 186 204 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 204 186 BsiHKAI G_wGCw'C Cuts at: 2026 2250 2412 2799 4014 4099 5170 2026 Size: 224 162 387 1215 85 1071 2809 Fragments arranged by size: 2809 1215 1071 387 224 162 85 BsmI GAATG_Cn' Cuts at: 3464 3557 3464 Size: 93 5860 BsmBI CGTCTCn'nnnn_ Cuts at: 2998 2998 Size: 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2250 2412 2799 2829 3272 4014 Size: 399 236 224 162 387 30 443 742 Cuts at: 4014 4099 5170 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 399 387 236 224 162 85 30 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2547 3308 844 Size: 1703 761 3489 Fragments arranged by size: 3489 1703 761 BsrBI CCG'CTC Cuts at: 1394 1823 3174 3840 1394 Size: 429 1351 666 3507 Fragments arranged by size: 3507 1351 666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4448 4622 65 Size: 1183 3200 174 1396 Fragments arranged by size: 3200 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2207 2213 4597 4784 1700 Size: 110 397 6 2384 187 2869 Fragments arranged by size: 2869 2384 397 187 110 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 2019 4013 5025 2019 Size: 1994 1012 2947 Fragments arranged by size: 2947 1994 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2476 794 Size: 1682 4271 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstYI r'GATC_y Cuts at: 1972 2608 3130 3283 3650 4035 4052 5504 5590 Size: 636 522 153 367 385 17 1452 86 Cuts at: 5590 5602 5948 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 636 522 385 367 346 153 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3792 Fragments arranged by size: 3792 1609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5214 Fragments arranged by size: 5214 683 56 BtsI GCAGTG_nn' Cuts at: 2297 2696 2774 3469 4263 4283 2297 Size: 399 78 695 794 20 3967 Fragments arranged by size: 3967 794 695 399 78 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2202 2590 2598 Size: 54 1425 50 275 18 372 388 8 Cuts at: 2598 2677 3400 4289 5918 8 Size: 79 723 889 1629 43 Fragments arranged by size: 1629 1425 889 723 388 372 275 79 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2202 3400 1537 Size: 275 390 1198 4090 Fragments arranged by size: 4090 1198 390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2700 2787 3883 1353 Size: 1347 87 1096 3423 Fragments arranged by size: 3423 1347 1096 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4540 4914 5060 387 Size: 1064 138 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2024 2248 2797 2024 Size: 224 549 5180 Fragments arranged by size: 5180 549 224 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 2777 4016 4613 5400 5668 1239 Size: 393 632 513 1239 597 787 268 1524 Fragments arranged by size: 1524 1239 787 632 597 513 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2506 2506 Size: 5953 EcoO109I rG'GnC_Cy Cuts at: 3110 3110 Size: 5953 EcoRI G'AATT_C Cuts at: 3262 3640 3262 Size: 378 5575 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 2018 2238 5100 1678 Size: 340 220 2862 2531 Fragments arranged by size: 2862 2531 340 220 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2600 2632 3009 3041 4594 Size: 32 135 32 1028 32 377 32 1553 Cuts at: 4594 4626 4668 4700 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1553 1028 377 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2619 3297 3543 669 Size: 988 281 681 678 246 3079 Fragments arranged by size: 3079 988 681 678 281 246 HindIII A'AGCT_T Cuts at: 748 2551 748 Size: 1803 4150 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KasI G'GCGC_C Cuts at: 2014 2014 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2367 2582 5067 5251 1305 Size: 1062 215 2485 184 2007 Fragments arranged by size: 2485 2007 1062 215 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2592 2679 10 Size: 54 1425 343 760 87 3284 Fragments arranged by size: 3284 1425 760 343 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2465 2822 3911 4270 4429 518 Size: 648 1299 357 1089 359 159 2042 Fragments arranged by size: 2042 1299 1089 648 359 357 159 MspA1I CmG'CkG Cuts at: 1606 2171 2900 2929 3369 4046 5194 5439 1606 Size: 565 729 29 440 677 1148 245 2120 Fragments arranged by size: 2120 1148 729 677 565 440 245 29 NaeI GCC'GGC Cuts at: 2209 2209 Size: 5953 NarI GG'CG_CC Cuts at: 2015 2015 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3792 Fragments arranged by size: 3792 1609 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NgoMIV G'CCGG_C Cuts at: 2207 2207 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 2202 3400 2202 Size: 1198 4755 NspI r_CATG'y Cuts at: 1132 1229 3150 3311 4856 1132 Size: 97 1921 161 1545 2229 Fragments arranged by size: 2229 1921 1545 161 97 PasI CC'CwG_GG Cuts at: 1348 2098 1348 Size: 750 5203 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 2755 1108 Size: 1647 4306 PfoI T'CCnGG_A Cuts at: 2334 2334 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3110 3110 Size: 5953 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PstI C_TGCA'G Cuts at: 830 2868 3305 830 Size: 2038 437 3478 Fragments arranged by size: 3478 2038 437 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 PvuII CAG'CTG Cuts at: 2900 2929 2900 Size: 29 5924 SacI G_AGCT'C Cuts at: 2026 2250 2799 2026 Size: 224 549 5180 Fragments arranged by size: 5180 549 224 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SapI GCTCTTCn'nnn_ Cuts at: 2787 2787 Size: 5953 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 3059 4201 5913 3059 Size: 1142 1712 3099 Fragments arranged by size: 3099 1712 1142 SfcI C'TryA_G Cuts at: 826 1045 2864 3301 4436 5117 5308 826 Size: 219 1819 437 1135 681 191 1471 Fragments arranged by size: 1819 1471 1135 681 437 219 191 SfoI GGC'GCC Cuts at: 2016 2016 Size: 5953 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 2213 1700 Size: 513 5440 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2107 2386 3053 4057 4958 5220 Size: 197 926 164 279 667 1004 901 262 Cuts at: 5220 820 Size: 1553 Fragments arranged by size: 1553 1004 926 901 667 279 262 197 164 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 2674 3070 3392 513 Size: 552 1609 396 322 3074 Fragments arranged by size: 3074 1609 552 396 322 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2697 3980 3997 4150 4335 4780 325 Size: 1255 1117 1283 17 153 185 445 1498 Fragments arranged by size: 1498 1283 1255 1117 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3057 4199 5911 Size: 274 80 33 51 1443 1080 1142 1712 Cuts at: 5911 96 Size: 138 Fragments arranged by size: 1712 1443 1142 1080 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5913 5945 5913 Size: 32 5921 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 4082 4082 Size: 5953 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI