Restriction map for
pFN21AB7449
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tru9I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AciI 421 CCGC CCGC(-3/-1)
AcsI 121 AAATTC [GA]^AATT[CT]
AcyI 13 GACGCC G[GA]^CG[CT]C
AocI 547 CCTGAGG CC^T[ACGT]AGG
ApoI 121 AAATTC [GA]^AATT[CT]
Asp700I 359 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 337 CCCGAG C^[CT]CG[GA]G
BbvI 516 GCAGC GCAGC(8/12)
BcnI 424 CCGGG CC^[GC]GG
BfaI 181 CTAG C^TAG
BglII 460 AGATCT A^GATCT
BmyI 417 GTGCCC G[AGT]GC[ACT]^C
BpmI 310 CTCCAG CTGGAG(16/14)
BsaHI 13 GACGCC G[GA]^CG[CT]C
BsaI 27 GAGACC GGTCTC(1/5)
BsmBI 8 GAGACG CGTCTC(1/5)
BsoBI 337 CCCGAG C^[CT]CG[GA]G
Bsp1286I 417 GTGCCC G[AGT]GC[ACT]^C
BssSI 454 CACGAG CACGAG(-5/-1)
BstBI 366 TTCGAA TT^CGAA
BstYI 460 AGATCT [GA]^GATC[CT]
Bsu36I 547 CCTGAGG CC^T[ACGT]AGG
DsaI 267 CCGTGG C^C[GA][CT]GG
Eco81I 547 CCTGAGG CC^T[ACGT]AGG
FauI 420 CCCGC CCCGC(4/6)
Fnu4HI 516 GCAGC GC^[ACGT]GC
HapII 424 CCGG C^CGG
HgaI 13 GACGC GACGC(5/10)
Hin1I 13 GACGCC G[GA]^CG[CT]C
HpaII 424 CCGG C^CGG
ItaI 516 GCAGC GC^[ACGT]GC
MaeI 181 CTAG C^TAG
MflI 460 AGATCT [GA]^GATC[CT]
MspI 424 CCGG C^CGG
NciI 424 CCGGG CC^[GC]GG
NdeI 148 CATATG CA^TATG
NspI 483 ACATGC [GA]CATG^[CT]
NspV 366 TTCGAA TT^CGAA
PleI 232 GACTC GAGTC(4/5)
SfuI 366 TTCGAA TT^CGAA
StuI 68 AGGCCT AGG^CCT
TseI 516 GCAGC G^C[AT]GC
Tsp45I 193 GTCAC ^GT[GC]AC
Tsp509I 122 AATT ^AATT
XhoII 460 AGATCT [GA]^GATC[CT]
XmnI 359 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 466 GGACC G^G[AT]CC
491 GGACC
BsiYI 469 CCCAAAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
470 CCAAAAGGGGG
BslI 469 CCCAAAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
470 CCAAAAGGGGG
BsmAI 8 GAGAC GTCTC(1/5)
27 GAGAC
BstF5I 321 CATCC GGATG(2/0)
36 GGATG
BstNI 32 CCAGG CC^[AT]GG
488 CCTGG
EcoRII 32 CCAGG ^CC[AT]GG
488 CCTGG
FokI 321 CATCC GGATG(9/13)
36 GGATG
HphI 522 GGTGA GGTGA(8/7)
194 TCACC
MaeII 191 ACGT A^CGT
285 ACGT
MaeIII 193 GTCAC ^GT[ACGT]AC
263 GTAAC
MspA1I 138 CAGCTG C[AC]G^C[GT]G
517 CAGCTG
MvaI 32 CCAGG CC^[AT]GG
488 CCTGG
MwoI 336 GCCCGAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
414 GCTGTGCCCGC
PvuII 138 CAGCTG CAG^CTG
517 CAGCTG
TaqI 74 TCGA T^CGA
367 TCGA
TthHB8I 74 TCGA T^CGA
367 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BcgI BsaJI BssKI Cac8I
Cfr13I DdeI DpnII EarI HaeIII
HinfI Ksp632I MboI MboII MnlI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TfiI