(Circular) (Six-base) MAPSORT of: pFN21AB7445.seq Check: 2779 from: 1 to: 6199 pFN21AB7445 6199 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2523 3542 1937 Size: 586 1019 4594 Fragments arranged by size: 4594 1019 586 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2047 2741 2826 4262 5646 5914 1632 Size: 415 694 85 1436 1384 268 1917 Fragments arranged by size: 1917 1436 1384 694 415 268 85 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6002 AflIII A'CryG_T Cuts at: 1203 5098 6075 1203 Size: 3895 977 1327 Fragments arranged by size: 3895 1327 977 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 4928 4928 Size: 6199 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6199 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6199 AlwNI CAG_nnn'CTG Cuts at: 3942 5514 3942 Size: 1572 4627 ApaI G_GGCC'C Cuts at: 2273 2273 Size: 6199 ApaLI G'TGCA_C Cuts at: 4256 5412 4256 Size: 1156 5043 ApoI r'AATT_y Cuts at: 1493 2648 3508 3734 3886 5995 1493 Size: 1155 860 226 152 2109 1697 Fragments arranged by size: 2109 1697 1155 860 226 152 AseI AT'TA_AT Cuts at: 160 1032 2540 4753 160 Size: 872 1508 2213 1606 Fragments arranged by size: 2213 1606 1508 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 3524 1870 Size: 73 1581 4545 Fragments arranged by size: 4545 1581 73 BamHI G'GATC_C Cuts at: 3529 3896 3529 Size: 367 5832 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3520 4975 618 Size: 325 217 627 1733 1455 1842 Fragments arranged by size: 1842 1733 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2273 3518 1391 Size: 882 1245 4072 Fragments arranged by size: 4072 1245 882 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2345 2741 928 Size: 971 446 396 4386 Fragments arranged by size: 4386 971 446 396 BbvCI CC'TCA_GC Cuts at: 2013 2013 Size: 6199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4390 4424 1903 Size: 34 2453 34 3678 Fragments arranged by size: 3678 2453 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4091 5301 4091 Size: 1210 4989 BclI T'GATC_A Cuts at: 1548 1548 Size: 6199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2947 4810 Size: 107 122 71 631 605 337 938 1863 Cuts at: 4810 136 Size: 1525 Fragments arranged by size: 1863 1525 938 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2197 6194 2197 Size: 3997 2202 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 2273 4260 5416 1790 Size: 483 1987 1156 2573 Fragments arranged by size: 2573 1987 1156 483 BmgBI CAC'GTC Cuts at: 1318 2185 1318 Size: 867 5332 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3930 4888 442 Size: 416 53 83 399 24 2513 958 1753 Fragments arranged by size: 2513 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3375 1056 Size: 2319 3880 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2267 2325 2424 2267 Size: 58 99 6042 Fragments arranged by size: 6042 99 58 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4859 1239 Size: 3620 2579 Bpu10I CC'TnA_GC Cuts at: 2013 2264 2013 Size: 251 5948 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2766 2973 4324 5189 5487 2766 Size: 207 1351 865 298 3478 Fragments arranged by size: 3478 1351 865 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2139 4862 882 Size: 617 640 2723 2219 Fragments arranged by size: 2723 2219 640 617 BsaAI yAC'GTr Cuts at: 493 1204 3990 493 Size: 711 2786 2702 Fragments arranged by size: 2786 2702 711 BsaBI GATnn'nnATC Cuts at: 2478 6193 2478 Size: 3715 2484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4006 4388 5023 275 Size: 53 83 186 936 2473 382 635 1451 Fragments arranged by size: 2473 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4632 5030 5304 5451 5862 4632 Size: 398 274 147 411 4969 Fragments arranged by size: 4969 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 1178 Size: 30 165 30 759 30 5185 Fragments arranged by size: 5185 759 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6199 BseYI C'CCAG_C Cuts at: 5402 5898 5402 Size: 496 5703 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5626 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3649 4410 5438 1540 Size: 275 186 1648 761 1028 2301 Fragments arranged by size: 2301 1648 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4260 4345 5416 4260 Size: 85 1071 5043 Fragments arranged by size: 5043 1071 85 BsmI GAATG_Cn' Cuts at: 3710 3803 3710 Size: 93 6106 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2273 3518 4260 4345 5416 1391 Size: 399 483 1245 742 85 1071 2174 Fragments arranged by size: 2174 1245 1071 742 483 399 85 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 4088 5818 4088 Size: 1730 4469 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3554 844 Size: 2710 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2246 4086 1394 Size: 429 423 1840 3507 Fragments arranged by size: 3507 1840 429 423 BsrDI GCAATG_nn' Cuts at: 65 1248 3109 3329 4694 4868 65 Size: 1183 1861 220 1365 174 1396 Fragments arranged by size: 1861 1396 1365 1183 220 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4843 5030 1700 Size: 110 3033 187 2869 Fragments arranged by size: 3033 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2620 96 Size: 2524 3675 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6199 BssSI C'ACGA_G Cuts at: 4259 5271 4259 Size: 1012 5187 BstAPI GCAn_nnn'nTGC Cuts at: 794 2085 794 Size: 1291 4908 BstBI TT'CG_AA Cuts at: 5003 5003 Size: 6199 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6199 BstYI r'GATC_y Cuts at: 1972 2197 2248 3529 3896 4281 4298 5750 5836 Size: 225 51 1281 367 385 17 1452 86 Cuts at: 5836 5848 6194 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1281 385 367 346 225 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4708 Fragments arranged by size: 4708 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5460 Fragments arranged by size: 5460 683 56 BtsI GCAGTG_nn' Cuts at: 2552 3715 4509 4529 2552 Size: 1163 794 20 4222 Fragments arranged by size: 4222 1163 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 2676 3503 2676 Size: 827 5372 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5206 809 Size: 507 3890 1802 Fragments arranged by size: 3890 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2544 3269 3646 Size: 54 1425 50 275 18 714 725 377 Cuts at: 3646 4535 6164 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 725 714 377 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2294 2893 2931 3174 4129 1353 Size: 941 599 38 243 955 3423 Fragments arranged by size: 3423 955 941 599 243 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4786 5160 5306 387 Size: 1064 138 3197 374 146 1280 Fragments arranged by size: 3197 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2047 2741 2826 4262 4859 5646 5914 Size: 393 415 694 85 1436 597 787 268 Cuts at: 5914 1239 Size: 1524 Fragments arranged by size: 1524 1436 787 694 597 415 393 268 85 EcoNI CCTnn'n_nnAGG Cuts at: 2107 2107 Size: 6199 EcoO109I rG'GnC_Cy Cuts at: 2269 2270 2269 Size: 1 6198 EcoRI G'AATT_C Cuts at: 3508 3886 3508 Size: 378 5821 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2071 2071 Size: 6199 FspI TGC'GCA Cuts at: 4705 4705 Size: 6199 HaeII r_GCGC'y Cuts at: 1678 2163 5346 1678 Size: 485 3183 2531 Fragments arranged by size: 3183 2531 485 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 4840 4872 4914 Size: 32 135 32 992 32 2244 32 42 Cuts at: 4914 4946 1373 Size: 32 2626 Fragments arranged by size: 2626 2244 992 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2375 3543 3789 669 Size: 988 281 437 1168 246 3079 Fragments arranged by size: 3079 1168 988 437 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6199 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5313 5497 1305 Size: 4008 184 2007 Fragments arranged by size: 4008 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2546 3271 10 Size: 54 1425 343 714 725 2938 Fragments arranged by size: 2938 1425 725 714 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 4157 4516 4675 518 Size: 648 1092 1899 359 159 2042 Fragments arranged by size: 2042 1899 1092 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3615 4292 5440 5685 1606 Size: 2009 677 1148 245 2120 Fragments arranged by size: 2120 2009 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4708 Fragments arranged by size: 4708 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NheI G'CTAG_C Cuts at: 1052 3371 1052 Size: 2319 3880 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 3557 5102 1132 Size: 97 2328 1545 2229 Fragments arranged by size: 2328 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6199 PciI A'CATG_T Cuts at: 5098 5098 Size: 6199 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6199 PfoI T'CCnGG_A Cuts at: 3387 3387 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4268 4300 5781 5813 4268 Size: 32 1481 32 4654 Fragments arranged by size: 4654 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6199 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PspOMI G'GGCC_C Cuts at: 2269 2269 Size: 6199 PstI C_TGCA'G Cuts at: 830 2111 3551 830 Size: 1281 1440 3478 Fragments arranged by size: 3478 1440 1281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 SalI G'TCGA_C Cuts at: 1936 3541 1936 Size: 1605 4594 SapI GCTCTTCn'nnn_ Cuts at: 2931 2931 Size: 6199 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 4447 6159 4447 Size: 1712 4487 SfcI C'TryA_G Cuts at: 826 1045 2107 3547 4682 5363 5554 826 Size: 219 1062 1440 1135 681 191 1471 Fragments arranged by size: 1471 1440 1135 1062 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 3526 1872 Size: 1654 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2781 2988 4303 5204 5466 820 Size: 197 926 838 207 1315 901 262 1553 Fragments arranged by size: 1553 1315 926 901 838 262 207 197 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 2767 4123 5 Size: 47 2715 1356 2081 Fragments arranged by size: 2715 2081 1356 47 StuI AGG'CCT Cuts at: 1517 3193 1517 Size: 1676 4523 StyI C'CwwG_G Cuts at: 513 1065 2004 2174 3638 513 Size: 552 939 170 1464 3074 Fragments arranged by size: 3074 1464 939 552 170 SwaI ATTT'AAAT Cuts at: 2676 2676 Size: 6199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 4226 4243 4396 4581 5026 325 Size: 1255 543 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 543 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2620 3487 4445 Size: 274 80 33 51 1443 643 867 958 Cuts at: 4445 6157 96 Size: 1712 138 Fragments arranged by size: 1712 1443 958 867 643 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6159 6191 6159 Size: 32 6167 Tth111I GACn'n_nGTC Cuts at: 1316 2370 1316 Size: 1054 5145 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 3524 1870 Size: 1654 4545 XmnI GAAnn'nnTTC Cuts at: 4328 4328 Size: 6199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI XcmI