Restriction map for
pFN21AB7444
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 962 GTCGAC GT^[AC][GT]AC
Alw44I 1205 GTGCAC G^TGCAC
ApaLI 1205 GTGCAC G^TGCAC
Asp700I 308 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 383 GGCACC G^G[CT][GA]CC
BbvI 190 GCTGC GCAGC(8/12)
BglII 328 AGATCT A^GATCT
BpmI 606 CTGGAG CTGGAG(16/14)
BsaBI 655 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 282 GAGACC GGTCTC(1/5)
BseRI 482 CTCCTC GAGGAG(10/8)
BsmI 186 GAATGC GAATGC(1/-1)
BspHI 1107 TCATGA T^CATGA
BsrI 445 CCAGT ACTGG(1/-1)
BstAPI 1207 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 328 AGATCT [GA]^GATC[CT]
Cac8I 23 GCAAGC GC[ACGT]^[ACGT]GC
DsaI 933 CCATGG C^C[GA][CT]GG
EarI 560 GAAGAG CTCTTC(1/4)
EcoNI 1213 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 190 GCTGC GC^[ACGT]GC
HindIII 25 AAGCTT A^AGCTT
ItaI 190 GCTGC GC^[ACGT]GC
Ksp632I 560 GAAGAG CTCTTC(1/4)
MaeII 211 ACGT A^CGT
MamI 655 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 328 AGATCT [GA]^GATC[CT]
NcoI 933 CCATGG C^CATGG
NdeI 1409 CATATG CA^TATG
PstI 173 CTGCAG CTGCA^G
RcaI 1107 TCATGA T^CATGA
SalI 962 GTCGAC G^TCGAC
TfiI 1365 GAATC G^A[AT]TC
TseI 190 GCTGC G^C[AT]GC
Tsp45I 552 GTCAC ^GT[GC]AC
Tsp509I 568 AATT ^AATT
TspRI 345 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 328 AGATCT [GA]^GATC[CT]
XmnI 308 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 1369 CCGC CCGC(-3/-1)
279 GCGG
AlwI 1396 GATCC GGATC(4/5)
123 GGATC
AspHI 1205 GTGCAC G[AT]GC[AT]^C
1387 GTGCTC
BsiHKAI 1205 GTGCAC G[AT]GC[AT]^C
1387 GTGCTC
BspMI 1212 ACCTGC ACCTGC(4/8)
1249 ACCTGC
BssSI 1271 CACGAG CACGAG(-5/-1)
1400 CTCGTG
HincII 624 GTCAAC GT[CT]^[GA]AC
962 GTCGAC
HindII 624 GTCAAC GT[CT]^[GA]AC
962 GTCGAC
HphI 1120 GGTGA GGTGA(8/7)
1315 GGTGA
MaeIII 552 GTCAC ^GT[ACGT]AC
1301 GTTAC
MseI 71 TTAA T^TAA
449 TTAA
NspI 620 GCATGT [GA]CATG^[CT]
1341 GCATGT
PaeR7I 1305 CTCGAG C^TCGAG
1476 CTCGAG
SfcI 173 CTGCAG C^T[GA][CT]AG
1436 CTATAG
Tru9I 71 TTAA T^TAA
449 TTAA
XhoI 1305 CTCGAG C^TCGAG
1476 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AluI AvaI AvaII BciVI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I EcoT22I FokI
HaeIII HinfI MaeI MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV NsiI PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TthHB8I XbaI