Restriction map for
pFN21AB7444
(Circular) (Six-base) MAPSORT of: pFN21AB7444.seq Check: 6149 from: 1 to: 6193
pFN21AB7444 6193 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3226 3263 3226
Size: 37 6156
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2968 3536 1937
Size: 1031 568 4594
Fragments arranged by size:
4594 1031 568
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2304 3170 4256 5640 5908 1632
Size: 672 866 1086 1384 268 1917
Fragments arranged by size:
1917 1384 1086 866 672 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 5092 6069 1203
Size: 3889 977 1327
Fragments arranged by size:
3889 1327 977
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 3936 5508 3936
Size: 1572 4621
ApaLI G'TGCA_C
Cuts at: 3210 4250 5406 3210
Size: 1040 1156 3997
Fragments arranged by size:
3997 1156 1040
ApoI r'AATT_y
Cuts at: 1493 3502 3728 3880 5989 1493
Size: 2009 226 152 2109 1697
Fragments arranged by size:
2109 2009 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2790 2901 3012 3310 3481 3518 1870
Size: 73 847 111 111 298 171 37 4545
Fragments arranged by size:
4545 847 298 171 111 111 73 37
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 3514 4969 618
Size: 325 217 627 601 1126 1455 1842
Fragments arranged by size:
1842 1455 1126 627 601 325 217
BanII G_rGCy'C
Cuts at: 1391 3512 1391
Size: 2121 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2399 2873 2936 4085 5295 2399
Size: 474 63 1149 1210 3297
Fragments arranged by size:
3297 1210 1149 474 63
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BfrBI ATG'CAT
Cuts at: 2720 2831 3053 2720
Size: 111 222 5860
Fragments arranged by size:
5860 222 111
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2333 6188 2333
Size: 3855 2338
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 2391 3214 4254 5410 1790
Size: 601 823 1040 1156 2573
Fragments arranged by size:
2573 1156 1040 823 601
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3924 4882 442
Size: 416 53 83 399 24 2507 958 1753
Fragments arranged by size:
2507 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3325 3325
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2632 4853 1239
Size: 1393 2221 2579
Fragments arranged by size:
2579 2221 1393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2405 3409 4318 5183 5481 2405
Size: 1004 909 865 298 3117
Fragments arranged by size:
3117 1004 909 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2281 4856 882
Size: 617 782 2575 2219
Fragments arranged by size:
2575 2219 782 617
BsaAI yAC'GTr
Cuts at: 493 1204 3984 493
Size: 711 2780 2702
Fragments arranged by size:
2780 2702 711
BsaBI GATnn'nnATC
Cuts at: 2664 6187 2664
Size: 3523 2670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4000 4382 5017 275
Size: 53 83 186 936 2467 382 635 1451
Fragments arranged by size:
2467 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5968
Fragments arranged by size:
5968 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2478 1186
Size: 1292 4901
BseYI C'CCAG_C
Cuts at: 5396 5892 5396
Size: 496 5697
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5620
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3214 3396 4254 4339 5410 3214
Size: 182 858 85 1071 3997
Fragments arranged by size:
3997 1071 858 182 85
BsmI GAATG_Cn'
Cuts at: 2197 3704 3797 2197
Size: 1507 93 4593
Fragments arranged by size:
4593 1507 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 3214 3396 3512 4254 4339 5410
Size: 399 601 823 182 116 742 85 1071
Cuts at: 5410 1391
Size: 2174
Fragments arranged by size:
2174 1071 823 742 601 399 182 116
85
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 3112 4082 5812 3112
Size: 970 1730 3493
Fragments arranged by size:
3493 1730 970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3226 3263 3548 844
Size: 2382 37 285 3489
Fragments arranged by size:
3489 2382 285 37
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4837 5024 1700
Size: 110 3027 187 2869
Fragments arranged by size:
3027 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 3276 3405 4253 5265 3276
Size: 129 848 1012 4204
Fragments arranged by size:
4204 1012 848 129
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3218 794
Size: 2424 3769
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstYI r'GATC_y
Cuts at: 1972 2333 3523 3890 4275 4292 5744 5830 5842
Size: 361 1190 367 385 17 1452 86 12
Cuts at: 5842 6188 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1190 385 367 361 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2938 513
Size: 552 939 934 3768
Fragments arranged by size:
3768 939 934 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 2356 3709 4503 4523 2356
Size: 1353 794 20 4026
Fragments arranged by size:
4026 1353 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3414 3449 529
Size: 35 2850 35 3273
Fragments arranged by size:
3273 2850 35 35
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3640 4529 6158
Size: 54 1425 50 275 18 1810 889 1629
Cuts at: 6158 8
Size: 43
Fragments arranged by size:
1810 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2560 4123 1353
Size: 1207 1563 3423
Fragments arranged by size:
3423 1563 1207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2299 3363 4780 5154 5300 387
Size: 1064 138 710 1064 1417 374 146 1280
Fragments arranged by size:
1417 1280 1064 1064 710 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 2632 3170 4256 4853 5640 5908
Size: 393 672 328 538 1086 597 787 268
Cuts at: 5908 1239
Size: 1524
Fragments arranged by size:
1524 1086 787 672 597 538 393 328
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3222 3222
Size: 6193
EcoO109I rG'GnC_Cy
Cuts at: 2483 2549 3130 3198 3227 2483
Size: 66 581 68 29 5449
Fragments arranged by size:
5449 581 68 66 29
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 5340 1678
Size: 3662 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 2605 2612 2637
Size: 32 135 32 541 32 460 7 25
Cuts at: 2637 2644 3449 3481 4834 4866 4908 4940 1373
Size: 7 805 32 1353 32 42 32 2626
Fragments arranged by size:
2626 1353 805 541 460 135 42 32
32 32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2631 2969 3537 3783 669
Size: 988 281 693 338 568 246 3079
Fragments arranged by size:
3079 988 693 568 338 281 246
HindIII A'AGCT_T
Cuts at: 748 2030 748
Size: 1282 4911
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2513 5307 5491 1305
Size: 1208 2794 184 2007
Fragments arranged by size:
2794 2007 1208 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4371
Fragments arranged by size:
4371 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2394 3159 3333 4151 4510 4669 518
Size: 648 1228 765 174 818 359 159 2042
Fragments arranged by size:
2042 1228 818 765 648 359 174 159
MspA1I CmG'CkG
Cuts at: 1606 3609 4286 5434 5679 1606
Size: 2003 677 1148 245 2120
Fragments arranged by size:
2120 2003 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2938 513
Size: 552 939 934 3768
Fragments arranged by size:
3768 939 934 552
NdeI CA'TA_TG
Cuts at: 387 3415 387
Size: 3028 3165
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NsiI A_TGCA'T
Cuts at: 2722 2833 3055 2722
Size: 111 222 5860
Fragments arranged by size:
5860 222 111
NspI r_CATG'y
Cuts at: 1132 1229 2629 3350 3551 5096 1132
Size: 97 1400 721 201 1545 2229
Fragments arranged by size:
2229 1545 1400 721 201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PfoI T'CCnGG_A
Cuts at: 2609 2609
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2483 2549 3198 2483
Size: 66 649 5478
Fragments arranged by size:
5478 649 66
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspXI vC'TCGA_Gb
Cuts at: 3310 3481 3310
Size: 171 6022
PstI C_TGCA'G
Cuts at: 830 2182 3545 830
Size: 1352 1363 3478
Fragments arranged by size:
3478 1363 1352
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 2967 3535 1936
Size: 1031 568 4594
Fragments arranged by size:
4594 1031 568
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2178 3441 3541 4676 5357 5548 826
Size: 219 1133 1263 100 1135 681 191 1471
Fragments arranged by size:
1471 1263 1135 1133 681 219 191 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 3310 3424 3481 4297 5198
Size: 197 926 477 890 114 57 816 901
Cuts at: 5198 5460 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 890 816 477 262 197
114 57
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2545 2938 3133 3632 513
Size: 552 939 541 393 195 499 3074
Fragments arranged by size:
3074 939 552 541 499 393 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4237 4390 4575 5020 325
Size: 1255 2640 17 153 185 445 1498
Fragments arranged by size:
2640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4439 6151 96
Size: 274 80 33 51 1443 2462 1712 138
Fragments arranged by size:
2462 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 2708 2762 2819 2873 2930 2984 3095 3529 2708
Size: 54 57 54 57 54 111 434 5372
Fragments arranged by size:
5372 434 111 57 57 54 54 54
XhoI C'TCGA_G
Cuts at: 1943 3310 3481 1943
Size: 1367 171 4655
Fragments arranged by size:
4655 1367 171
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 2317 4322 2317
Size: 2005 4188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI