Restriction map for
pFN21AB7444
(Linear) (Six-base) MAPSORT of: KIBB7444.seq Check: 7812 from: 1 to: 1488
KIBB7444.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1221 1258 1488
Size: 1221 37 230
Fragments arranged by size:
1221 230 37
AccI GT'mk_AC
Cuts at: 0 963 1488
Size: 963 525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1165 1488
Size: 299 866 323
Fragments arranged by size:
866 323 299
ApaLI G'TGCA_C
Cuts at: 0 1205 1488
Size: 1205 283
AvaI C'yCGr_G
Cuts at: 0 785 896 1007 1305 1476 1488
Size: 785 111 111 298 171 12
Fragments arranged by size:
785 298 171 111 111 12
BanI G'GyrC_C
Cuts at: 0 383 1488
Size: 383 1105
BciVI GTATCCnnnnn_n'
Cuts at: 0 394 868 931 1488
Size: 394 474 63 557
Fragments arranged by size:
557 474 394 63
BfrBI ATG'CAT
Cuts at: 0 715 826 1048 1488
Size: 715 111 222 440
Fragments arranged by size:
715 440 222 111
BglII A'GATC_T
Cuts at: 0 328 1488
Size: 328 1160
Bme1580I G_kGCm'C
Cuts at: 0 386 1209 1488
Size: 386 823 279
Fragments arranged by size:
823 386 279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1320 1488
Size: 1320 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1488
Size: 627 861
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1404 1488
Size: 400 1004 84
Fragments arranged by size:
1004 400 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 276 1488
Size: 276 1212
BsaBI GATnn'nnATC
Cuts at: 0 659 1488
Size: 659 829
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 473 1488
Size: 473 1015
BsiHKAI G_wGCw'C
Cuts at: 0 1209 1391 1488
Size: 1209 182 97
Fragments arranged by size:
1209 182 97
BsmI GAATG_Cn'
Cuts at: 0 192 1488
Size: 192 1296
Bsp1286I G_dGCh'C
Cuts at: 0 386 1209 1391 1488
Size: 386 823 182 97
Fragments arranged by size:
823 386 182 97
BspHI T'CATG_A
Cuts at: 0 1107 1488
Size: 1107 381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1221 1258 1488
Size: 1221 37 230
Fragments arranged by size:
1221 230 37
BssSI C'ACGA_G
Cuts at: 0 1271 1400 1488
Size: 1271 129 88
Fragments arranged by size:
1271 129 88
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1213 1488
Size: 1213 275
BstYI r'GATC_y
Cuts at: 0 328 1488
Size: 328 1160
BtgI C'CryG_G
Cuts at: 0 933 1488
Size: 933 555
BtsI GCAGTG_nn'
Cuts at: 0 351 1488
Size: 351 1137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1409 1444 1488
Size: 1409 35 44
Fragments arranged by size:
1409 44 35
EarI CTCTTCn'nnn_
Cuts at: 0 555 1488
Size: 555 933
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 294 1358 1488
Size: 294 1064 130
Fragments arranged by size:
1064 294 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 627 1165 1488
Size: 299 328 538 323
Fragments arranged by size:
538 328 323 299
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1217 1488
Size: 1217 271
EcoO109I rG'GnC_Cy
Cuts at: 0 478 544 1125 1193 1222 1488
Size: 478 66 581 68 29 266
Fragments arranged by size:
581 478 266 68 66 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 600 607 632 639 1444 1476
Size: 108 32 460 7 25 7 805 32
Cuts at: 1476 1488
Size: 12
Fragments arranged by size:
805 460 108 32 32 25 12 7
7
HincII GTy'rAC
Cuts at: 0 626 964 1488
Size: 626 338 524
Fragments arranged by size:
626 524 338
HindIII A'AGCT_T
Cuts at: 0 25 1488
Size: 25 1463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1488
Size: 508 980
MslI CAynn'nnrTG
Cuts at: 0 389 1154 1328 1488
Size: 389 765 174 160
Fragments arranged by size:
765 389 174 160
NcoI C'CATG_G
Cuts at: 0 933 1488
Size: 933 555
NdeI CA'TA_TG
Cuts at: 0 1410 1488
Size: 1410 78
NsiI A_TGCA'T
Cuts at: 0 717 828 1050 1488
Size: 717 111 222 438
Fragments arranged by size:
717 438 222 111
NspI r_CATG'y
Cuts at: 0 624 1345 1488
Size: 624 721 143
Fragments arranged by size:
721 624 143
PfoI T'CCnGG_A
Cuts at: 0 604 1488
Size: 604 884
PpuMI rG'GwC_Cy
Cuts at: 0 478 544 1193 1488
Size: 478 66 649 295
Fragments arranged by size:
649 478 295 66
PspXI vC'TCGA_Gb
Cuts at: 0 1305 1476 1488
Size: 1305 171 12
Fragments arranged by size:
1305 171 12
PstI C_TGCA'G
Cuts at: 0 177 1488
Size: 177 1311
SalI G'TCGA_C
Cuts at: 0 962 1488
Size: 962 526
SfcI C'TryA_G
Cuts at: 0 173 1436 1488
Size: 173 1263 52
Fragments arranged by size:
1263 173 52
SmlI C'TyrA_G
Cuts at: 0 415 1305 1419 1476 1488
Size: 415 890 114 57 12
Fragments arranged by size:
890 415 114 57 12
StyI C'CwwG_G
Cuts at: 0 540 933 1128 1488
Size: 540 393 195 360
Fragments arranged by size:
540 393 360 195
XbaI T'CTAG_A
Cuts at: 0 703 757 814 868 925 979 1090 1488
Size: 703 54 57 54 57 54 111 398
Fragments arranged by size:
703 398 111 57 57 54 54 54
XhoI C'TCGA_G
Cuts at: 0 1305 1476 1488
Size: 1305 171 12
Fragments arranged by size:
1305 171 12
XmnI GAAnn'nnTTC
Cuts at: 0 312 1488
Size: 312 1176
Enzymes that do cut:
AarI AccI AcuI ApaLI AvaI BanI BciVI BfrBI
BglII Bme1580I BplI BpmI BpuEI BsaI BsaBI BseRI
BsiHKAI BsmI Bsp1286I BspHI BspMI BssSI BstAPI BstYI
BtgI BtsI CspCI EarI EciI Eco57MI EcoNI EcoO109I
Hin4I HincII HindIII MmeI MslI NcoI NdeI NsiI
NspI PfoI PpuMI PspXI PstI SalI SfcI SmlI
StyI XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BclI BglI BlpI BmgBI BmrI BmtI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII
DrdI EaeI EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XcmI XmaI ZraI