Restriction map for
pFN21AB7442
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
Acc65I AccI AccII AccIII AciI
AclI AflIII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsgI BsiEI BsiWI BsoBI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I CpoI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MluI MroI MslI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 527 GACGTC GACGT^C
AcsI 1021 AAATTT [GA]^AATT[CT]
AcyI 527 GACGTC G[GA]^CG[CT]C
AflII 717 CTTAAG C^TTAAG
AhdI 522 GACCAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 259 GTGCAC G^TGCAC
AlwNI 803 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 259 GTGCAC G^TGCAC
ApoI 1021 AAATTT [GA]^AATT[CT]
Asp700I 105 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 522 GACCAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 359 TGGCCA TGG^CCA
BbsI 927 GAAGAC GAAGAC(2/6)
BfaI 626 CTAG C^TAG
BfrI 717 CTTAAG C^TTAAG
BpuAI 927 GAAGAC GAAGAC(2/6)
BsaBI 585 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 527 GACGTC G[GA]^CG[CT]C
BsaI 839 GGTCTC GGTCTC(1/5)
BsmBI 529 CGTCTC CGTCTC(1/5)
BsmI 445 GAATGC GAATGC(1/-1)
Bsp1407I 290 TGTACA T^GTACA
BspDI 476 ATCGAT AT^CGAT
BsrDI 236 GCAATG GCAATG(2/0)
BsrGI 290 TGTACA T^GTACA
ClaI 476 ATCGAT AT^CGAT
DraI 24 TTTAAA TTT^AAA
EaeI 359 TGGCCA [CT]^GGCC[GA]
Eam1105I 522 GACCAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 431 CCTTGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
Hin1I 527 GACGTC G[GA]^CG[CT]C
HincII 595 GTCAAC GT[CT]^[GA]AC
HindII 595 GTCAAC GT[CT]^[GA]AC
MaeI 626 CTAG C^TAG
MaeII 528 ACGT A^CGT
MamI 585 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 552 CAATTG C^AATTG
MluNI 359 TGGCCA TGG^CCA
MscI 359 TGGCCA TGG^CCA
MspA1I 806 CAGCTG C[AC]G^C[GT]G
MunI 552 CAATTG C^AATTG
NsiI 1 ATGCAT ATGCA^T
NspI 353 GCATGC [GA]CATG^[CT]
PvuII 806 CAGCTG CAG^CTG
SapI 201 GCTCTTC GCTCTTC(1/4)
ScaI 876 AGTACT AGT^ACT
SfcI 440 CTACAG C^T[GA][CT]AG
SmlI 717 CTTAAG C^T[CT][GA]AG
SphI 353 GCATGC GCATG^C
SspBI 290 TGTACA T^GTACA
StyI 431 CCTTGG C^C[AT][AT]GG
SwaI 23 ATTTAAAT ATTT^AAAT
TaqI 477 TCGA T^CGA
TthHB8I 477 TCGA T^CGA
XmnI 105 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 199 GTGCTC G[AT]GC[AT]^C
259 GTGCAC
AvaII 761 GGTCC G^G[AT]CC
795 GGTCC
BanII 435 GGGCTC G[GA]GC[CT]^C
894 GAGCCC
BbvI 805 GCAGC GCAGC(8/12)
401 GCTGC
BpmI 129 CTCCAG CTGGAG(16/14)
409 CTCCAG
BseRI 190 CTCCTC GAGGAG(10/8)
659 CTCCTC
BsiHKAI 199 GTGCTC G[AT]GC[AT]^C
259 GTGCAC
BsmFI 346 GTCCC GGGAC(10/14)
796 GTCCC
BstF5I 150 CATCC GGATG(2/0)
294 CATCC
BstXI 350 CCAGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
539 CCAATCTTGTGG
BstYI 185 AGATCC [GA]^GATC[CT]
622 GGATCT
Fnu4HI 401 GCTGC GC^[ACGT]GC
805 GCAGC
FokI 150 CATCC GGATG(9/13)
294 CATCC
HphI 335 TCACC GGTGA(8/7)
843 TCACC
ItaI 401 GCTGC GC^[ACGT]GC
805 GCAGC
MflI 185 AGATCC [GA]^GATC[CT]
622 GGATCT
PleI 275 GACTC GAGTC(4/5)
936 GACTC
PpuMI 760 GGGTCCT [GA]G^G[AT]CC[CT]
794 GGGTCCC
TfiI 39 GATTC G^A[AT]TC
585 GATTC
TseI 401 GCTGC G^C[AT]GC
805 GCAGC
XhoII 185 AGATCC [GA]^GATC[CT]
622 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AlwI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
HaeIII HinfI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI Tru9I Tsp45I Tsp509I
TspRI