(Circular) (Six-base) MAPSORT of: pFN21AB7442.seq Check: 8740 from: 1 to: 5740 pFN21AB7442 5740 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2536 3550 278 Size: 53 83 186 936 1000 1014 2468 Fragments arranged by size: 2468 1014 1000 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2790 3041 3803 5187 5455 1632 Size: 1158 251 762 1384 268 1917 Fragments arranged by size: 1917 1384 1158 762 268 251 AflII C'TTAA_G Cuts at: 820 1017 2722 820 Size: 197 1705 3838 Fragments arranged by size: 3838 1705 197 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 2532 4469 2532 Size: 1937 3803 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AlwNI CAG_nnn'CTG Cuts at: 2813 3483 5055 2813 Size: 670 1572 3498 Fragments arranged by size: 3498 1572 670 ApaLI G'TGCA_C Cuts at: 2264 3797 4953 2264 Size: 1533 1156 3051 Fragments arranged by size: 3051 1533 1156 ApoI r'AATT_y Cuts at: 1493 3026 3049 3275 3427 5536 1493 Size: 1533 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 1533 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 3065 1870 Size: 73 1122 4545 Fragments arranged by size: 4545 1122 73 BamHI G'GATC_C Cuts at: 3070 3437 3070 Size: 367 5373 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3061 4516 618 Size: 325 217 627 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 627 325 217 BanII G_rGCy'C Cuts at: 1391 2444 2903 3059 1391 Size: 1053 459 156 4072 Fragments arranged by size: 4072 1053 459 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2939 928 Size: 971 1040 3729 Fragments arranged by size: 3729 1040 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3931 3965 1903 Size: 34 50 34 1910 34 3678 Fragments arranged by size: 3678 1910 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3632 4842 3632 Size: 1210 4530 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4351 136 Size: 107 122 71 631 605 2679 1525 Fragments arranged by size: 2679 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 2268 3801 4957 1790 Size: 478 1533 1156 2573 Fragments arranged by size: 2573 1533 1156 478 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2278 3471 4429 Size: 416 53 83 399 24 861 1193 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1193 958 861 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3033 3033 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2119 2399 4400 1239 Size: 880 280 2001 2579 Fragments arranged by size: 2579 2001 880 280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2850 4403 882 Size: 617 1351 1553 2219 Fragments arranged by size: 2219 1553 1351 617 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 2594 5734 2594 Size: 3140 2600 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2533 3547 3929 4564 Size: 53 83 186 936 1000 1014 382 635 Cuts at: 4564 275 Size: 1451 Fragments arranged by size: 1451 1014 1000 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4173 4571 4845 4992 5403 4173 Size: 398 274 147 411 4510 Fragments arranged by size: 4510 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5515 Fragments arranged by size: 5515 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2655 1186 Size: 1000 469 4271 Fragments arranged by size: 4271 1000 469 BseYI C'CCAG_C Cuts at: 2126 2354 2562 2659 2813 4943 5439 2126 Size: 228 208 97 154 2130 496 2427 Fragments arranged by size: 2427 2130 496 228 208 154 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5167 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 2268 3801 3886 4957 2208 Size: 60 1533 85 1071 2991 Fragments arranged by size: 2991 1533 1071 85 60 BsmI GAATG_Cn' Cuts at: 2456 3251 3344 2456 Size: 795 93 4852 Fragments arranged by size: 4852 795 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2540 2540 Size: 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2268 2444 2903 3059 3801 3886 Size: 399 418 60 176 459 156 742 85 Cuts at: 3886 4957 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 459 418 399 176 156 85 60 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3095 844 Size: 2251 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2248 4235 4409 65 Size: 1183 1000 1987 174 1396 Fragments arranged by size: 1987 1396 1183 1000 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4384 4571 1700 Size: 110 2574 187 2869 Fragments arranged by size: 2869 2574 187 110 BsrGI T'GTAC_A Cuts at: 96 2295 96 Size: 2199 3541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstXI CCAn_nnnn'nTGG Cuts at: 2362 2551 2362 Size: 189 5551 BstYI r'GATC_y Cuts at: 1972 2190 2627 3070 3437 3822 3839 5291 5377 Size: 218 437 443 367 385 17 1452 86 Cuts at: 5377 5389 5735 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 443 437 385 367 346 218 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 2966 3256 4050 4070 2966 Size: 290 794 20 4636 Fragments arranged by size: 4636 794 290 20 ClaI AT'CG_AT Cuts at: 1554 2482 1554 Size: 928 4812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 2031 3044 2031 Size: 1013 4727 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 3187 4076 Size: 54 1425 50 275 18 534 823 889 Cuts at: 4076 5705 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 823 534 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 2673 2759 3670 1353 Size: 860 460 86 911 3423 Fragments arranged by size: 3423 911 860 460 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2399 2790 3041 3803 4400 5187 Size: 393 487 280 391 251 762 597 787 Cuts at: 5187 5455 1239 Size: 268 1524 Fragments arranged by size: 1524 787 762 597 487 393 391 280 268 251 EcoO109I rG'GnC_Cy Cuts at: 2693 2766 2800 2693 Size: 73 34 5633 Fragments arranged by size: 5633 73 34 EcoRI G'AATT_C Cuts at: 3049 3427 3049 Size: 378 5362 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2844 2844 Size: 5740 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 4887 1678 Size: 3209 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2035 2067 2518 2550 2926 Size: 32 135 32 463 32 451 32 376 Cuts at: 2926 2958 2974 3006 4381 4413 4455 4487 1373 Size: 32 16 32 1375 32 42 32 2626 Fragments arranged by size: 2626 1375 463 451 376 135 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2602 3084 3330 669 Size: 988 281 664 482 246 3079 Fragments arranged by size: 3079 988 664 482 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 2557 3339 2557 Size: 782 4958 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2540 4854 5038 1305 Size: 1235 2314 184 2007 Fragments arranged by size: 2314 2007 1235 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 10 Size: 54 1425 343 534 3384 Fragments arranged by size: 3384 1425 534 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3698 4057 4216 518 Size: 648 2532 359 159 2042 Fragments arranged by size: 2532 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2813 3156 3833 4981 5226 1606 Size: 1207 343 677 1148 245 2120 Fragments arranged by size: 2120 1207 1148 677 343 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5188 NdeI CA'TA_TG Cuts at: 387 387 Size: 5740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 2362 3098 4643 1132 Size: 97 1133 736 1545 2229 Fragments arranged by size: 2229 1545 1133 736 97 PasI CC'CwG_GG Cuts at: 1348 2178 1348 Size: 830 4910 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5740 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2766 2800 2766 Size: 34 5706 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PstI C_TGCA'G Cuts at: 830 3092 830 Size: 2262 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2813 2813 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SanDI GG'GwC_CC Cuts at: 2800 2800 Size: 5740 SapI GCTCTTCn'nnn_ Cuts at: 2213 2213 Size: 5740 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 2883 3988 5700 2883 Size: 1105 1712 2923 Fragments arranged by size: 2923 1712 1105 SfcI C'TryA_G Cuts at: 826 1045 2445 3088 4223 4904 5095 826 Size: 219 1400 643 1135 681 191 1471 Fragments arranged by size: 1471 1400 1135 681 643 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 3067 1872 Size: 1195 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2722 3844 4745 5007 820 Size: 197 926 779 1122 901 262 1553 Fragments arranged by size: 1553 1122 926 901 779 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 2362 3098 1132 Size: 1230 736 3774 Fragments arranged by size: 3774 1230 736 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2436 3179 513 Size: 552 1371 743 3074 Fragments arranged by size: 3074 1371 743 552 SwaI ATTT'AAAT Cuts at: 2031 2031 Size: 5740 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3767 3784 3937 4122 4567 325 Size: 1255 2187 17 153 185 445 1498 Fragments arranged by size: 2187 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 2881 3986 Size: 274 80 33 51 1443 318 586 1105 Cuts at: 3986 5698 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1105 586 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5740 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 3065 1870 Size: 1195 4545 XmnI GAAnn'nnTTC Cuts at: 2114 3869 2114 Size: 1755 3985 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2534 3548 276 Size: 53 83 186 936 1000 1014 2468 Fragments arranged by size: 2468 1014 1000 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI XcmI