(Circular) (Six-base) MAPSORT of: pFN21AB7441.seq Check: 5530 from: 1 to: 5350 pFN21AB7441 5350 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2604 3160 278 Size: 53 83 186 936 1068 556 2468 Fragments arranged by size: 2468 1068 936 556 186 83 53 AccI GT'mk_AC Cuts at: 1937 2178 2693 1937 Size: 241 515 4594 Fragments arranged by size: 4594 515 241 Acc65I G'GTAC_C Cuts at: 2671 2671 Size: 5350 AclI AA'CG_TT Cuts at: 1563 3477 3850 1563 Size: 1914 373 3063 Fragments arranged by size: 3063 1914 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3413 4797 5065 1632 Size: 1781 1384 268 1917 Fragments arranged by size: 1917 1781 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5153 AflIII A'CryG_T Cuts at: 1203 2059 4249 5226 1203 Size: 856 2190 977 1327 Fragments arranged by size: 2190 1327 977 856 AgeI A'CCGG_T Cuts at: 4181 4181 Size: 5350 AhdI GACnn_n'nnGTC Cuts at: 4079 4079 Size: 5350 AleI CACnn'nnGTG Cuts at: 1166 2060 1166 Size: 894 4456 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2052 1184 Size: 868 4482 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2369 2401 2369 Size: 32 5318 AlwNI CAG_nnn'CTG Cuts at: 3093 4665 3093 Size: 1572 3778 ApaLI G'TGCA_C Cuts at: 2008 2063 3407 4563 2008 Size: 55 1344 1156 2795 Fragments arranged by size: 2795 1344 1156 55 ApoI r'AATT_y Cuts at: 1493 2659 2885 3037 5146 1493 Size: 1166 226 152 2109 1697 Fragments arranged by size: 2109 1697 1166 226 152 AseI AT'TA_AT Cuts at: 160 1032 2619 3904 160 Size: 872 1587 1285 1606 Fragments arranged by size: 1606 1587 1285 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 AvaI C'yCGr_G Cuts at: 1870 1943 2675 1870 Size: 73 732 4545 Fragments arranged by size: 4545 732 73 BamHI G'GATC_C Cuts at: 2680 3047 2680 Size: 367 4983 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2671 4126 618 Size: 325 217 627 884 1455 1842 Fragments arranged by size: 1842 1455 884 627 325 217 BanII G_rGCy'C Cuts at: 1391 2105 2598 2669 1391 Size: 714 493 71 4072 Fragments arranged by size: 4072 714 493 71 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4379 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3541 3575 1903 Size: 34 1604 34 3678 Fragments arranged by size: 3678 1604 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3242 4452 3242 Size: 1210 4140 BclI T'GATC_A Cuts at: 1548 2551 1548 Size: 1003 4347 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3961 136 Size: 107 122 71 631 605 2289 1525 Fragments arranged by size: 2289 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2033 5345 2033 Size: 3312 2038 BlpI GC'TnA_GC Cuts at: 2767 2767 Size: 5350 Bme1580I G_kGCm'C Cuts at: 1790 2012 2067 3411 4567 1790 Size: 222 55 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 222 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3081 4039 442 Size: 416 53 83 399 24 1664 958 1753 Fragments arranged by size: 1753 1664 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 4010 1239 Size: 1340 1431 2579 Fragments arranged by size: 2579 1431 1340 Bpu10I CC'TnA_GC Cuts at: 2268 2268 Size: 5350 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2440 3475 4340 4638 2440 Size: 1035 865 298 3152 Fragments arranged by size: 3152 1035 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2195 4013 882 Size: 617 696 1818 2219 Fragments arranged by size: 2219 1818 696 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 3141 493 Size: 711 1170 767 2702 Fragments arranged by size: 2702 1170 767 711 BsaBI GATnn'nnATC Cuts at: 5344 5344 Size: 5350 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2601 3157 3539 4174 Size: 53 83 186 936 1068 556 382 635 Cuts at: 4174 275 Size: 1451 Fragments arranged by size: 1451 1068 936 635 556 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3783 4181 4455 4602 5013 3783 Size: 398 274 147 411 4120 Fragments arranged by size: 4120 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5125 Fragments arranged by size: 5125 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5350 BseYI C'CCAG_C Cuts at: 4553 5049 4553 Size: 496 4854 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4777 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2800 3561 4589 1540 Size: 275 186 799 761 1028 2301 Fragments arranged by size: 2301 1028 799 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2067 2174 2598 3411 3496 4567 2012 Size: 55 107 424 813 85 1071 2795 Fragments arranged by size: 2795 1071 813 424 107 85 55 BsmI GAATG_Cn' Cuts at: 2861 2954 2861 Size: 93 5257 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2067 2105 2174 2598 2669 3411 Size: 399 222 55 38 69 424 71 742 Cuts at: 3411 3496 4567 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 222 85 71 69 55 38 BspEI T'CCGG_A Cuts at: 5013 5013 Size: 5350 BspHI T'CATG_A Cuts at: 2629 3239 4969 2629 Size: 610 1730 3010 Fragments arranged by size: 3010 1730 610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2705 844 Size: 1861 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3237 1394 Size: 429 1414 3507 Fragments arranged by size: 3507 1414 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2436 2483 3845 4019 65 Size: 1183 1188 47 1362 174 1396 Fragments arranged by size: 1396 1362 1188 1183 174 47 BsrFI r'CCGG_y Cuts at: 1700 1810 3994 4181 1700 Size: 110 2184 187 2869 Fragments arranged by size: 2869 2184 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5350 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5350 BssSI C'ACGA_G Cuts at: 3410 4422 3410 Size: 1012 4338 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5350 BstBI TT'CG_AA Cuts at: 4154 4154 Size: 5350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5350 BstXI CCAn_nnnn'nTGG Cuts at: 2097 2097 Size: 5350 BstYI r'GATC_y Cuts at: 1972 2033 2680 3047 3432 3449 4901 4987 4999 Size: 61 647 367 385 17 1452 86 12 Cuts at: 4999 5345 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 647 385 367 346 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 2111 2185 513 Size: 552 1046 74 3678 Fragments arranged by size: 3678 1046 552 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4611 Fragments arranged by size: 4611 683 56 BtsI GCAGTG_nn' Cuts at: 2500 2501 2866 3660 3680 2500 Size: 1 365 794 20 4170 Fragments arranged by size: 4170 794 365 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5315 DraI TTT'AAA Cuts at: 2654 2654 Size: 5350 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4357 809 Size: 507 3041 1802 Fragments arranged by size: 3041 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2797 3686 5315 Size: 54 1425 50 275 18 967 889 1629 Cuts at: 5315 8 Size: 43 Fragments arranged by size: 1629 1425 967 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2797 1537 Size: 275 985 4090 Fragments arranged by size: 4090 985 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2226 3280 1353 Size: 873 1054 3423 Fragments arranged by size: 3423 1054 873 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3937 4311 4457 387 Size: 1064 138 2348 374 146 1280 Fragments arranged by size: 2348 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2596 2596 Size: 5350 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2579 3413 4010 4797 5065 1239 Size: 393 947 834 597 787 268 1524 Fragments arranged by size: 1524 947 834 787 597 393 268 EcoRI G'AATT_C Cuts at: 2659 3037 2659 Size: 378 4972 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2293 2293 Size: 5350 FspI TGC'GCA Cuts at: 3856 3856 Size: 5350 HaeII r_GCGC'y Cuts at: 1678 4497 1678 Size: 2819 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2482 2514 3991 4023 4065 Size: 32 135 32 910 32 1477 32 42 Cuts at: 4065 4097 1373 Size: 32 2626 Fragments arranged by size: 2626 1477 910 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2694 2940 669 Size: 988 281 756 246 3079 Fragments arranged by size: 3079 988 756 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5350 HpaI GTT'AAC Cuts at: 2940 2940 Size: 5350 KpnI G_GTAC'C Cuts at: 2675 2675 Size: 5350 MfeI C'AATT_G Cuts at: 2949 2949 Size: 5350 MluI A'CGCG_T Cuts at: 5226 5226 Size: 5350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4464 4648 1305 Size: 3159 184 2007 Fragments arranged by size: 3159 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3528 Fragments arranged by size: 3528 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2076 2082 2216 3308 3667 3826 Size: 648 894 16 6 134 1092 359 159 Cuts at: 3826 518 Size: 2042 Fragments arranged by size: 2042 1092 894 648 359 159 134 16 6 MspA1I CmG'CkG Cuts at: 1606 2557 2648 2766 3443 4591 4836 1606 Size: 951 91 118 677 1148 245 2120 Fragments arranged by size: 2120 1148 951 677 245 118 91 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4798 NdeI CA'TA_TG Cuts at: 387 387 Size: 5350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5350 NotI GC'GGCC_GC Cuts at: 2797 2797 Size: 5350 NspI r_CATG'y Cuts at: 1132 1229 2063 2221 2461 2708 4253 1132 Size: 97 834 158 240 247 1545 2229 Fragments arranged by size: 2229 1545 834 247 240 158 97 PasI CC'CwG_GG Cuts at: 1348 2587 1348 Size: 1239 4111 PciI A'CATG_T Cuts at: 2059 4249 2059 Size: 2190 3160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5350 PmeI GTTT'AAAC Cuts at: 2654 2654 Size: 5350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3419 3451 4932 4964 3419 Size: 32 1481 32 3805 Fragments arranged by size: 3805 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5350 PsiI TTA'TAA Cuts at: 2920 2920 Size: 5350 PstI C_TGCA'G Cuts at: 830 2319 2507 2702 830 Size: 1489 188 195 3478 Fragments arranged by size: 3478 1489 195 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5350 PvuII CAG'CTG Cuts at: 2557 2648 2557 Size: 91 5259 SacI G_AGCT'C Cuts at: 2598 2598 Size: 5350 SalI G'TCGA_C Cuts at: 1936 2692 1936 Size: 756 4594 SbfI CC_TGCA'GG Cuts at: 2702 2702 Size: 5350 ScaI AGT'ACT Cuts at: 3598 5310 3598 Size: 1712 3638 SfcI C'TryA_G Cuts at: 826 1045 2315 2503 2698 3833 4514 4705 826 Size: 219 1270 188 195 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 681 219 195 191 188 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5350 SmaI CCC'GGG Cuts at: 1872 2677 1872 Size: 805 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2419 3454 4355 4617 820 Size: 197 926 476 1035 901 262 1553 Fragments arranged by size: 1553 1035 926 901 476 262 197 SnaBI TAC'GTA Cuts at: 493 3141 493 Size: 2648 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5350 SphI G_CATG'C Cuts at: 1132 2708 1132 Size: 1576 3774 SspI AAT'ATT Cuts at: 5 52 3274 5 Size: 47 3222 2081 Fragments arranged by size: 3222 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5350 StyI C'CwwG_G Cuts at: 513 1065 2789 513 Size: 552 1724 3074 Fragments arranged by size: 3074 1724 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3377 3394 3547 3732 4177 325 Size: 1255 1797 17 153 185 445 1498 Fragments arranged by size: 1797 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3596 5308 96 Size: 274 80 33 51 1443 1619 1712 138 Fragments arranged by size: 1712 1619 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5310 5342 5310 Size: 32 5318 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5350 XbaI T'CTAG_A Cuts at: 2686 2686 Size: 5350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2584 2359 Size: 225 5125 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5350 XmaI C'CCGG_G Cuts at: 1870 2675 1870 Size: 805 4545 XmnI GAAnn'nnTTC Cuts at: 3479 3479 Size: 5350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2602 3158 276 Size: 53 83 186 936 1068 556 2468 Fragments arranged by size: 2468 1068 936 556 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI