Restriction map for
pFN21AB7440
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MaeIII MamI
MboI MfeI MflI MluNI MroI
MscI MseI MunI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI Sau3AI
SbfI SexAI SfaNI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcyI 142 GGCGCC G[GA]^CG[CT]C
AflIII 368 ACGCGT A^C[GA][CT]GT
AspHI 385 GAGCTC G[AT]GC[AT]^C
AvaI 146 CCCGAG C^[CT]CG[GA]G
BanI 142 GGCGCC G^G[CT][GA]CC
BanII 385 GAGCTC G[GA]GC[CT]^C
BbeI 142 GGCGCC GGCGC^C
BbrPI 440 CACGTG CAC^GTG
BcnI 35 CCCGG CC^[GC]GG
BfaI 376 CTAG C^TAG
BpmI 244 CTGGAG CTGGAG(16/14)
BsaAI 440 CACGTG [CT]AC^GT[GA]
BsaHI 142 GGCGCC G[GA]^CG[CT]C
BsaJI 197 CCATGG C^C[ACGT][ACGT]GG
BsiHKAI 385 GAGCTC G[AT]GC[AT]^C
BsiWI 68 CGTACG C^GTACG
BsmAI 351 GAGAC GTCTC(1/5)
BsoBI 146 CCCGAG C^[CT]CG[GA]G
BsrFI 139 GCCGGC [GA]^CCGG[CT]
BsrI 432 CCAGT ACTGG(1/-1)
BstF5I 396 CATCC GGATG(2/0)
BstXI 427 CCAGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 139 GCCGGC [GA]^CCGG[CT]
DraII 239 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 197 CCATGG C^C[GA][CT]GG
EarI 382 GAAGAG CTCTTC(1/4)
EcoNI 74 CCTTAGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 239 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 197 CCATGG C^C[AT][AT]GG
FokI 396 CATCC GGATG(9/13)
Hin1I 142 GGCGCC G[GA]^CG[CT]C
HincII 63 GTTGAC GT[CT]^[GA]AC
HindII 63 GTTGAC GT[CT]^[GA]AC
HinfI 305 GAGTC G^A[ACGT]TC
HphI 363 GGTGA GGTGA(8/7)
KasI 142 GGCGCC G^GCGCC
Ksp632I 382 GAAGAG CTCTTC(1/4)
MaeI 376 CTAG C^TAG
MboII 382 GAAGA GAAGA(8/7)
MluI 368 ACGCGT A^CGCGT
MslI 394 CACATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 139 GCCGGC GCC^GGC
NarI 142 GGCGCC GG^CGCC
NciI 35 CCCGG CC^[GC]GG
NcoI 197 CCATGG C^CATGG
NgoMIV 139 GCCGGC G^CCGGC
NspI 255 GCATGT [GA]CATG^[CT]
PleI 305 GAGTC GAGTC(4/5)
PmaCI 440 CACGTG CAC^GTG
PmlI 440 CACGTG CAC^GTG
PpuMI 239 GGGACCT [GA]G^G[AT]CC[CT]
PstI 232 CTGCAG CTGCA^G
PvuII 183 CAGCTG CAG^CTG
SacI 385 GAGCTC GAGCT^C
SapI 382 GAAGAGC GCTCTTC(1/4)
ScaI 55 AGTACT AGT^ACT
SfoI 142 GGCGCC GGC^GCC
SmlI 43 CTTGAG C^T[CT][GA]AG
StyI 197 CCATGG C^C[AT][AT]GG
TaqI 329 TCGA T^CGA
TspRI 433 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 329 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 56 GTAC GT^AC
69 GTAC
AvaII 21 GGACC G^G[AT]CC
240 GGACC
BmyI 385 GAGCTC G[AGT]GC[ACT]^C
437 GGGCAC
BsmFI 239 GGGAC GGGAC(10/14)
162 GTCCC
Bsp1286I 385 GAGCTC G[AGT]GC[ACT]^C
437 GGGCAC
Cfr13I 21 GGACC G^G[ACGT]CC
240 GGACC
DdeI 75 CTTAG C^T[ACGT]AG
205 CTCAG
EaeI 137 TGGCCG [CT]^GGCC[GA]
320 CGGCCA
FauI 390 CCCGC CCCGC(4/6)
237 GCGGG
HaeII 142 GGCGCC [GA]GCGC^[CT]
208 AGCGCC
HaeIII 138 GGCC GG^CC
321 GGCC
HapII 36 CCGG C^CGG
140 CCGG
HpaII 36 CCGG C^CGG
140 CCGG
MaeII 67 ACGT A^CGT
441 ACGT
MspI 36 CCGG C^CGG
140 CCGG
RsaI 56 GTAC GT^AC
69 GTAC
Sau96I 21 GGACC G^G[ACGT]CC
240 GGACC
SfcI 232 CTGCAG C^T[GA][CT]AG
294 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccII AciI AluI BbvI BsiYI
BslI BssKI BstNI BstUI Cac8I
CfoI EcoRII Fnu4HI HhaI HinP1I
ItaI MnlI MspA1I MvaI MvnI
MwoI NlaIII NlaIV ScrFI TseI